Macrophomina phaseolina tobamo-like virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; unclassified Virgaviridae

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088CPQ6|A0A088CPQ6_9VIRU Methyltransferase/RNA helicase OS=Macrophomina phaseolina tobamo-like virus OX=1527523 PE=4 SV=1
MM1 pKa = 7.12SQQIKK6 pKa = 10.8CPVCQKK12 pKa = 11.1DD13 pKa = 3.69FDD15 pKa = 4.33RR16 pKa = 11.84KK17 pKa = 9.27QAEE20 pKa = 3.94LHH22 pKa = 5.81IAEE25 pKa = 4.8AHH27 pKa = 5.88SSPDD31 pKa = 3.23KK32 pKa = 11.22KK33 pKa = 10.44EE34 pKa = 4.05WPDD37 pKa = 3.65LPDD40 pKa = 4.14GNTLTEE46 pKa = 4.03AEE48 pKa = 4.98FIRR51 pKa = 11.84MALEE55 pKa = 4.22KK56 pKa = 9.93PQEE59 pKa = 3.91IGVAIDD65 pKa = 3.37QPVTVVPPSTIFEE78 pKa = 5.11DD79 pKa = 3.14IGIKK83 pKa = 10.16VDD85 pKa = 4.07DD86 pKa = 4.42FQHH89 pKa = 7.53VDD91 pKa = 2.75ITTMAGATLTSSEE104 pKa = 3.98AKK106 pKa = 9.74MLFNVLEE113 pKa = 3.85EE114 pKa = 4.11HH115 pKa = 6.92LAFKK119 pKa = 10.48INRR122 pKa = 11.84AAFVANFIDD131 pKa = 3.31WGIRR135 pKa = 11.84TSFTEE140 pKa = 4.0EE141 pKa = 3.9LEE143 pKa = 4.31EE144 pKa = 5.23AGGFSVASNPVSGKK158 pKa = 8.99LQEE161 pKa = 4.68RR162 pKa = 11.84YY163 pKa = 6.71MTVLEE168 pKa = 3.84MHH170 pKa = 7.06KK171 pKa = 10.41IITEE175 pKa = 3.85YY176 pKa = 10.36FAAKK180 pKa = 8.58GTGRR184 pKa = 11.84SFTVRR189 pKa = 11.84RR190 pKa = 11.84FGRR193 pKa = 11.84FLAPKK198 pKa = 9.96IPDD201 pKa = 2.99ICDD204 pKa = 3.14QVEE207 pKa = 4.21KK208 pKa = 11.11LKK210 pKa = 10.34MYY212 pKa = 7.6YY213 pKa = 7.71TTGSPMSRR221 pKa = 11.84RR222 pKa = 11.84LGVRR226 pKa = 11.84NNLFLTVTSIYY237 pKa = 10.71EE238 pKa = 4.01YY239 pKa = 10.46IKK241 pKa = 10.55PFNKK245 pKa = 7.59WTRR248 pKa = 11.84DD249 pKa = 3.13EE250 pKa = 4.06KK251 pKa = 11.31AAWEE255 pKa = 4.04ALNRR259 pKa = 11.84TVTKK263 pKa = 10.3QPRR266 pKa = 11.84AQDD269 pKa = 3.49TSFVPQDD276 pKa = 3.58LRR278 pKa = 11.84PRR280 pKa = 11.84PYY282 pKa = 9.8EE283 pKa = 4.01AEE285 pKa = 4.08TLVEE289 pKa = 4.13TRR291 pKa = 11.84STDD294 pKa = 3.4FARR297 pKa = 11.84RR298 pKa = 11.84HH299 pKa = 5.64DD300 pKa = 4.31TDD302 pKa = 3.39PLKK305 pKa = 10.98AVFGQPAAVQGGYY318 pKa = 10.85GKK320 pKa = 10.57GAEE323 pKa = 4.3IFEE326 pKa = 4.31KK327 pKa = 10.25MRR329 pKa = 11.84TGSSGGKK336 pKa = 8.81NAA338 pKa = 4.61

Molecular weight: 38.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088CPZ9|A0A088CPZ9_9VIRU RNA helicase OS=Macrophomina phaseolina tobamo-like virus OX=1527523 PE=4 SV=1
MM1 pKa = 7.51AFKK4 pKa = 10.67YY5 pKa = 10.57RR6 pKa = 11.84NNPKK10 pKa = 10.12SFSLPRR16 pKa = 11.84EE17 pKa = 4.2FVAHH21 pKa = 6.22FRR23 pKa = 11.84EE24 pKa = 4.49SQNVDD29 pKa = 2.5TDD31 pKa = 3.37RR32 pKa = 11.84TLFGFQVLFTKK43 pKa = 9.46TKK45 pKa = 9.36KK46 pKa = 10.64KK47 pKa = 8.91EE48 pKa = 3.88WLEE51 pKa = 4.2GGHH54 pKa = 6.81APVTFLRR61 pKa = 11.84FFRR64 pKa = 11.84EE65 pKa = 3.87FGQWVKK71 pKa = 10.9GFSAKK76 pKa = 9.76EE77 pKa = 3.46RR78 pKa = 11.84GAAAIAATKK87 pKa = 10.11HH88 pKa = 6.03KK89 pKa = 10.85GVGLYY94 pKa = 9.42PRR96 pKa = 11.84RR97 pKa = 11.84GVYY100 pKa = 9.78RR101 pKa = 11.84DD102 pKa = 3.15NGDD105 pKa = 3.41YY106 pKa = 10.94LGTYY110 pKa = 8.92QEE112 pKa = 4.54VVSEE116 pKa = 4.15GKK118 pKa = 9.7IDD120 pKa = 3.72PTLLAMGMIDD130 pKa = 3.71VLDD133 pKa = 3.89SSVIEE138 pKa = 4.37SSTSQTSQATSSTTTSKK155 pKa = 10.75KK156 pKa = 10.05ASKK159 pKa = 10.21KK160 pKa = 10.45KK161 pKa = 10.82SFTANSAKK169 pKa = 9.87TSSARR174 pKa = 11.84VTHH177 pKa = 6.49AQAKK181 pKa = 7.83DD182 pKa = 3.53TTQKK186 pKa = 11.14AQVLDD191 pKa = 3.44TSIQKK196 pKa = 8.32RR197 pKa = 11.84TSYY200 pKa = 11.03SQFNLGDD207 pKa = 3.51RR208 pKa = 11.84QRR210 pKa = 11.84PLSFTEE216 pKa = 3.48VHH218 pKa = 5.94VPVARR223 pKa = 11.84GNYY226 pKa = 9.5RR227 pKa = 11.84GVTQKK232 pKa = 10.13IVAGPPGGYY241 pKa = 10.15VITGPTGCGKK251 pKa = 8.87STVALLPLFSGKK263 pKa = 10.37SSVLIVEE270 pKa = 4.64PTQANAANIFHH281 pKa = 6.87EE282 pKa = 4.91FSNVLPTLHH291 pKa = 6.66EE292 pKa = 4.68AGVIPWNVPPVEE304 pKa = 4.36FTAPTTRR311 pKa = 11.84EE312 pKa = 3.68GSYY315 pKa = 11.31GPLSVTTTDD324 pKa = 3.44KK325 pKa = 11.02LLEE328 pKa = 4.03YY329 pKa = 10.35FEE331 pKa = 5.0WKK333 pKa = 10.51GSLPKK338 pKa = 9.97VDD340 pKa = 4.17YY341 pKa = 11.21LIIDD345 pKa = 4.2EE346 pKa = 4.48FHH348 pKa = 6.92LPIPSMVQTVEE359 pKa = 3.96LLRR362 pKa = 11.84TFALVPKK369 pKa = 9.42YY370 pKa = 10.51IFVSATAVGYY380 pKa = 9.8SVNPEE385 pKa = 3.75LPKK388 pKa = 10.75AVTQTWGQLPIGQIPSKK405 pKa = 10.61MEE407 pKa = 4.12GSDD410 pKa = 3.47LDD412 pKa = 3.53PRR414 pKa = 11.84RR415 pKa = 11.84WWKK418 pKa = 10.84RR419 pKa = 11.84GDD421 pKa = 3.43GNVAVVAPSVIVAKK435 pKa = 10.55RR436 pKa = 11.84LFNVYY441 pKa = 10.07RR442 pKa = 11.84DD443 pKa = 3.42WQIRR447 pKa = 11.84AFLITRR453 pKa = 11.84EE454 pKa = 4.39TFVSEE459 pKa = 3.95YY460 pKa = 9.96MKK462 pKa = 10.83AATNYY467 pKa = 10.21RR468 pKa = 11.84SMTTFVLEE476 pKa = 4.39PGVEE480 pKa = 4.1AGVTLCIDD488 pKa = 3.48VLII491 pKa = 4.86

Molecular weight: 54.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

4565

338

2128

1141.3

127.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.375 ± 0.429

1.358 ± 0.252

6.243 ± 0.649

5.608 ± 0.266

5.016 ± 0.177

6.813 ± 0.229

1.752 ± 0.034

4.951 ± 0.171

5.849 ± 0.222

8.105 ± 0.414

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.928 ± 0.17

3.264 ± 0.174

4.666 ± 0.348

3.22 ± 0.221

6.396 ± 0.274

7.579 ± 0.25

6.813 ± 0.623

9.332 ± 0.445

1.205 ± 0.112

3.505 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski