Vibrio phage vB_VmeM-32

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; unclassified Tevenvirinae

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 254 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U4IFW7|A0A0U4IFW7_9CAUD Marine proteobacterial sortase target protein OS=Vibrio phage vB_VmeM-32 OX=1775142 GN=VmeM32_00069 PE=4 SV=1
MM1 pKa = 7.45EE2 pKa = 4.08VLIRR6 pKa = 11.84LVFGAIKK13 pKa = 10.55GLITFIPLALTLWVFSLILSLSFGLNEE40 pKa = 4.96FIITAAFCYY49 pKa = 10.11GFGIGSVVIEE59 pKa = 4.49YY60 pKa = 10.86DD61 pKa = 4.97DD62 pKa = 6.6DD63 pKa = 5.52IDD65 pKa = 4.58DD66 pKa = 3.87

Molecular weight: 7.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U4IGI3|A0A0U4IGI3_9CAUD Portal protein OS=Vibrio phage vB_VmeM-32 OX=1775142 GN=VmeM32_00005 PE=3 SV=1
MM1 pKa = 7.5SSLVLSQLKK10 pKa = 10.17LAVSGARR17 pKa = 11.84LVSNMKK23 pKa = 9.84YY24 pKa = 10.37DD25 pKa = 3.35KK26 pKa = 10.28KK27 pKa = 9.38TGKK30 pKa = 9.99FVLTRR35 pKa = 11.84INDD38 pKa = 3.63TAILRR43 pKa = 11.84KK44 pKa = 9.71SGAYY48 pKa = 9.84FIVVDD53 pKa = 4.1SAAISFNLFGASKK66 pKa = 8.86TPRR69 pKa = 11.84GFMDD73 pKa = 3.31VVKK76 pKa = 10.49RR77 pKa = 11.84LNNSVSGQNAQLADD91 pKa = 3.6KK92 pKa = 10.66LKK94 pKa = 10.8LVSNRR99 pKa = 11.84IKK101 pKa = 9.84RR102 pKa = 11.84DD103 pKa = 2.99SSIRR107 pKa = 11.84VMYY110 pKa = 9.38MPYY113 pKa = 10.04SALRR117 pKa = 11.84SMLEE121 pKa = 3.79HH122 pKa = 6.86EE123 pKa = 5.2GFNGINDD130 pKa = 3.72STEE133 pKa = 3.76LARR136 pKa = 11.84KK137 pKa = 9.25LRR139 pKa = 11.84EE140 pKa = 3.6TYY142 pKa = 9.97IFHH145 pKa = 6.99GRR147 pKa = 11.84AKK149 pKa = 10.45SS150 pKa = 3.24

Molecular weight: 16.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

254

0

254

59151

37

1260

232.9

26.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.002 ± 0.175

1.187 ± 0.072

6.668 ± 0.141

6.397 ± 0.173

4.455 ± 0.107

5.669 ± 0.203

2.064 ± 0.09

7.887 ± 0.148

7.249 ± 0.211

7.861 ± 0.116

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.387 ± 0.081

7.129 ± 0.169

2.742 ± 0.085

3.33 ± 0.089

4.602 ± 0.107

7.248 ± 0.149

6.172 ± 0.185

6.614 ± 0.121

1.173 ± 0.053

4.164 ± 0.123
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski