Psychromonas sp. RZ22

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Psychromonadaceae; Psychromonas; unclassified Psychromonas

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2647 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y7ZS86|A0A4Y7ZS86_9GAMM Uncharacterized protein OS=Psychromonas sp. RZ22 OX=2555643 GN=E2R68_10975 PE=4 SV=1
MM1 pKa = 7.16SFKK4 pKa = 11.08YY5 pKa = 10.18NALALITASLLVTACGSSSSSSDD28 pKa = 3.19NGNTTEE34 pKa = 5.84DD35 pKa = 4.02GEE37 pKa = 4.59QEE39 pKa = 3.65ITSNYY44 pKa = 6.75EE45 pKa = 3.91TVEE48 pKa = 3.86LDD50 pKa = 3.31ATDD53 pKa = 3.69GSTAVQLDD61 pKa = 4.19LASGAIVTDD70 pKa = 4.44DD71 pKa = 3.61SWHH74 pKa = 6.51LAYY77 pKa = 10.19QKK79 pKa = 11.29YY80 pKa = 10.64LGFKK84 pKa = 10.62LNGGSSGDD92 pKa = 3.71AGVSGCIAHH101 pKa = 7.2EE102 pKa = 4.01YY103 pKa = 11.01SSIFDD108 pKa = 3.44STGASIEE115 pKa = 4.55DD116 pKa = 3.8EE117 pKa = 4.57FKK119 pKa = 11.26LLTVSSTEE127 pKa = 3.89DD128 pKa = 3.12SFVAVTADD136 pKa = 2.98SCTDD140 pKa = 3.77FVEE143 pKa = 5.42DD144 pKa = 3.87SVATFIEE151 pKa = 4.41TEE153 pKa = 3.72EE154 pKa = 4.32WLDD157 pKa = 3.26ADD159 pKa = 4.18YY160 pKa = 11.53SAGAPVYY167 pKa = 9.93GAKK170 pKa = 10.37AGNGWIIRR178 pKa = 11.84SADD181 pKa = 2.9GATYY185 pKa = 10.73GRR187 pKa = 11.84VSVNSVEE194 pKa = 4.53VVFGASTTRR203 pKa = 11.84KK204 pKa = 9.62LVFNSEE210 pKa = 3.99LWNGSVFEE218 pKa = 4.22TAQLSPEE225 pKa = 4.37LDD227 pKa = 3.61FSEE230 pKa = 5.07DD231 pKa = 3.09QVYY234 pKa = 9.95WDD236 pKa = 5.03LEE238 pKa = 4.55TNSLVSATDD247 pKa = 3.13DD248 pKa = 3.5WEE250 pKa = 4.58LSVSVNGRR258 pKa = 11.84DD259 pKa = 3.47YY260 pKa = 11.08PLQINGGASGTGQAGVGAVLSDD282 pKa = 4.08DD283 pKa = 4.18LASVTDD289 pKa = 3.93PTDD292 pKa = 2.99TAQVYY297 pKa = 10.14KK298 pKa = 10.71YY299 pKa = 10.39FADD302 pKa = 3.77TASGVLSGPGTYY314 pKa = 10.61GPLQYY319 pKa = 11.08AVAGGHH325 pKa = 6.39LMWPTFTTYY334 pKa = 10.92LIKK337 pKa = 10.63DD338 pKa = 3.31AQDD341 pKa = 3.18RR342 pKa = 11.84LFKK345 pKa = 10.77VQVVSNYY352 pKa = 9.22GANGNSASGNLVLRR366 pKa = 11.84YY367 pKa = 10.07EE368 pKa = 4.32EE369 pKa = 4.71LNN371 pKa = 3.55

Molecular weight: 39.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y7ZVI7|A0A4Y7ZVI7_9GAMM LysR family transcriptional regulator OS=Psychromonas sp. RZ22 OX=2555643 GN=E2R68_09655 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNTKK11 pKa = 10.0RR12 pKa = 11.84KK13 pKa = 8.73RR14 pKa = 11.84SHH16 pKa = 6.16GFRR19 pKa = 11.84TRR21 pKa = 11.84MATKK25 pKa = 10.18NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.5GRR39 pKa = 11.84ASLSAA44 pKa = 3.83

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2647

0

2647

880803

19

3594

332.8

37.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.065 ± 0.051

1.047 ± 0.016

5.433 ± 0.045

6.105 ± 0.045

4.354 ± 0.035

6.349 ± 0.043

2.192 ± 0.027

7.36 ± 0.048

6.14 ± 0.049

10.671 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.402 ± 0.023

4.807 ± 0.038

3.599 ± 0.025

4.605 ± 0.044

3.821 ± 0.037

6.686 ± 0.04

5.55 ± 0.043

6.581 ± 0.044

1.1 ± 0.017

3.133 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski