Ammonifex degensii (strain DSM 10501 / KC4)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacteraceae; Moorella group; Ammonifex; Ammonifex degensii

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2077 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C9RCV7|C9RCV7_AMMDK Uncharacterized protein OS=Ammonifex degensii (strain DSM 10501 / KC4) OX=429009 GN=Adeg_0948 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 9.67WRR4 pKa = 11.84CQVCGYY10 pKa = 10.25IYY12 pKa = 10.54DD13 pKa = 4.23PEE15 pKa = 4.52EE16 pKa = 4.5GNPPDD21 pKa = 4.16IPPGIEE27 pKa = 4.12FEE29 pKa = 4.48GLPEE33 pKa = 4.54DD34 pKa = 4.44WVCPTCGADD43 pKa = 3.14KK44 pKa = 11.0SYY46 pKa = 11.25FEE48 pKa = 4.59PLRR51 pKa = 5.04

Molecular weight: 5.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C9RAM3|C9RAM3_AMMDK ATP-dependent DNA helicase RecG OS=Ammonifex degensii (strain DSM 10501 / KC4) OX=429009 GN=recG PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.46QPKK8 pKa = 8.26RR9 pKa = 11.84KK10 pKa = 9.02HH11 pKa = 6.01RR12 pKa = 11.84KK13 pKa = 8.16RR14 pKa = 11.84VHH16 pKa = 5.23GFLRR20 pKa = 11.84RR21 pKa = 11.84MRR23 pKa = 11.84TPGGRR28 pKa = 11.84RR29 pKa = 11.84VLKK32 pKa = 10.27RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2077

0

2077

626053

30

1340

301.4

33.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.699 ± 0.06

1.278 ± 0.026

3.957 ± 0.031

8.148 ± 0.069

3.834 ± 0.041

8.188 ± 0.049

1.754 ± 0.02

4.57 ± 0.049

4.797 ± 0.045

11.915 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.714 ± 0.02

2.238 ± 0.027

5.345 ± 0.043

2.843 ± 0.026

7.984 ± 0.069

4.49 ± 0.036

4.376 ± 0.04

8.578 ± 0.049

1.418 ± 0.026

2.875 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski