Lachnospiraceae bacterium 9_1_43BFAA

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2717 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F3AMA3|F3AMA3_9FIRM PS_pyruv_trans domain-containing protein OS=Lachnospiraceae bacterium 9_1_43BFAA OX=658088 GN=HMPREF0987_01577 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 9.93IAKK5 pKa = 8.09KK6 pKa = 8.09TAAFLLVLFLGIQCMPLTALAADD29 pKa = 4.12GTLSFSDD36 pKa = 3.69PTAKK40 pKa = 10.39AGEE43 pKa = 4.37TIEE46 pKa = 4.14VTVKK50 pKa = 10.39INTAEE55 pKa = 4.18GPIGDD60 pKa = 4.01GQVQVTYY67 pKa = 10.78DD68 pKa = 3.36PTYY71 pKa = 10.52LEE73 pKa = 4.49FVSGVNATGEE83 pKa = 4.25AGTITLSASGNGIDD97 pKa = 5.3SEE99 pKa = 4.61LAYY102 pKa = 11.49SMTFKK107 pKa = 11.06ALAEE111 pKa = 4.48GEE113 pKa = 4.45TTLQAVGYY121 pKa = 6.33TAYY124 pKa = 9.76TFGGEE129 pKa = 4.21EE130 pKa = 4.32LNLTLGTSAVKK141 pKa = 10.21IEE143 pKa = 4.93AGDD146 pKa = 3.83GTQPAAAQQPGTQTMIEE163 pKa = 4.33LDD165 pKa = 3.35GKK167 pKa = 10.55QYY169 pKa = 10.87FIADD173 pKa = 5.41DD174 pKa = 3.76IPEE177 pKa = 4.08ALIPEE182 pKa = 5.19GYY184 pKa = 9.53EE185 pKa = 3.95KK186 pKa = 10.93SQLTYY191 pKa = 9.99EE192 pKa = 4.92GSPHH196 pKa = 6.81TILVHH201 pKa = 5.9TGSGQKK207 pKa = 9.39MIYY210 pKa = 10.67LMDD213 pKa = 3.72TQGEE217 pKa = 4.75GNLFLYY223 pKa = 10.53DD224 pKa = 3.73SEE226 pKa = 4.61TDD228 pKa = 3.12HH229 pKa = 7.18FSAFEE234 pKa = 4.5KK235 pKa = 10.25IDD237 pKa = 3.99LSSDD241 pKa = 3.48SYY243 pKa = 11.85ILITDD248 pKa = 4.03DD249 pKa = 4.85KK250 pKa = 10.07EE251 pKa = 4.36SANIPEE257 pKa = 5.03DD258 pKa = 3.85YY259 pKa = 10.91EE260 pKa = 4.09EE261 pKa = 4.07TTIQINGKK269 pKa = 8.42VFPAWQNVVDD279 pKa = 4.09TKK281 pKa = 11.2LADD284 pKa = 3.54FCLVYY289 pKa = 10.81AVDD292 pKa = 3.76NAGKK296 pKa = 10.22KK297 pKa = 10.15GLYY300 pKa = 9.92QYY302 pKa = 11.59DD303 pKa = 4.06STQKK307 pKa = 8.86TYY309 pKa = 10.86QRR311 pKa = 11.84FLPPTAEE318 pKa = 4.31KK319 pKa = 10.18EE320 pKa = 4.24KK321 pKa = 10.73QDD323 pKa = 3.18GSVANKK329 pKa = 7.72VTTFVEE335 pKa = 4.27EE336 pKa = 4.1NLPKK340 pKa = 10.46IMVGAWGVFLMMLLLIVILAVKK362 pKa = 9.86LRR364 pKa = 11.84HH365 pKa = 6.14RR366 pKa = 11.84NEE368 pKa = 4.12EE369 pKa = 3.66LDD371 pKa = 4.09DD372 pKa = 4.83YY373 pKa = 11.62YY374 pKa = 11.44DD375 pKa = 3.56QEE377 pKa = 4.23YY378 pKa = 11.24DD379 pKa = 3.65EE380 pKa = 5.02NPRR383 pKa = 11.84AARR386 pKa = 11.84GFSSEE391 pKa = 5.54DD392 pKa = 3.38YY393 pKa = 11.1DD394 pKa = 5.18EE395 pKa = 6.52SDD397 pKa = 4.85DD398 pKa = 5.26DD399 pKa = 5.42DD400 pKa = 5.18YY401 pKa = 11.81DD402 pKa = 4.19YY403 pKa = 11.92EE404 pKa = 4.18EE405 pKa = 6.18DD406 pKa = 3.98YY407 pKa = 11.64DD408 pKa = 4.79EE409 pKa = 6.65SDD411 pKa = 4.67DD412 pKa = 5.26DD413 pKa = 5.42DD414 pKa = 5.18YY415 pKa = 11.81DD416 pKa = 4.19YY417 pKa = 11.92EE418 pKa = 4.15EE419 pKa = 6.12DD420 pKa = 3.77YY421 pKa = 11.62DD422 pKa = 4.17EE423 pKa = 6.19SEE425 pKa = 4.36EE426 pKa = 4.91DD427 pKa = 3.45DD428 pKa = 4.0YY429 pKa = 11.9AYY431 pKa = 10.47EE432 pKa = 4.15DD433 pKa = 4.73AYY435 pKa = 11.13DD436 pKa = 4.03EE437 pKa = 5.65SEE439 pKa = 5.05DD440 pKa = 5.8DD441 pKa = 4.26YY442 pKa = 11.96DD443 pKa = 5.53DD444 pKa = 5.99DD445 pKa = 4.69YY446 pKa = 11.8DD447 pKa = 4.34YY448 pKa = 11.95EE449 pKa = 4.2EE450 pKa = 5.84DD451 pKa = 4.09YY452 pKa = 11.21EE453 pKa = 5.69SEE455 pKa = 4.16DD456 pKa = 4.92AYY458 pKa = 11.25DD459 pKa = 5.31DD460 pKa = 4.47LLDD463 pKa = 4.93YY464 pKa = 11.55YY465 pKa = 11.17DD466 pKa = 6.09DD467 pKa = 5.61DD468 pKa = 6.36DD469 pKa = 6.7DD470 pKa = 5.98YY471 pKa = 11.85EE472 pKa = 4.6LEE474 pKa = 4.74RR475 pKa = 11.84EE476 pKa = 4.36ILDD479 pKa = 3.54EE480 pKa = 5.53DD481 pKa = 3.64EE482 pKa = 5.29GFFDD486 pKa = 4.62KK487 pKa = 10.95RR488 pKa = 11.84SGKK491 pKa = 9.8KK492 pKa = 9.97RR493 pKa = 11.84GIDD496 pKa = 3.79LII498 pKa = 4.39

Molecular weight: 55.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F3APH0|F3APH0_9FIRM Fumerase_C domain-containing protein OS=Lachnospiraceae bacterium 9_1_43BFAA OX=658088 GN=HMPREF0987_02344 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 4.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2717

0

2717

842039

22

3638

309.9

34.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.124 ± 0.046

1.494 ± 0.019

5.073 ± 0.035

8.558 ± 0.062

4.199 ± 0.029

7.032 ± 0.042

1.775 ± 0.022

7.446 ± 0.045

7.277 ± 0.043

9.033 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.151 ± 0.024

4.014 ± 0.032

3.166 ± 0.025

3.491 ± 0.031

4.44 ± 0.033

5.461 ± 0.037

5.253 ± 0.034

6.98 ± 0.04

0.913 ± 0.016

4.119 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski