Micromonospora rosaria

Taxonomy: cellular organisms;

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5974 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A136PWW5|A0A136PWW5_9ACTN RNA helicase OS=Micromonospora rosaria OX=47874 GN=AWW66_05780 PE=4 SV=1
MM1 pKa = 7.26VNNSKK6 pKa = 10.05IDD8 pKa = 3.7DD9 pKa = 3.68EE10 pKa = 4.35NAIVFQQQPSQPADD24 pKa = 3.35MYY26 pKa = 11.5SLAWLSKK33 pKa = 8.09MCHH36 pKa = 6.49AGTSITFKK44 pKa = 9.15WTIDD48 pKa = 3.49YY49 pKa = 11.04NFVWGQTGTLKK60 pKa = 10.8PGVDD64 pKa = 3.82YY65 pKa = 10.78EE66 pKa = 4.34AGQIIPADD74 pKa = 3.78LQNQNMVTLSYY85 pKa = 10.59IDD87 pKa = 4.52DD88 pKa = 4.03GFEE91 pKa = 4.21FGTPTSGAAPGSLLITEE108 pKa = 5.18DD109 pKa = 3.48EE110 pKa = 4.53TVPGPGLATQGSVGIGMSGAGTFVVPTQPKK140 pKa = 8.93TGAQFLLTPTYY151 pKa = 8.81WLAFGSYY158 pKa = 10.08KK159 pKa = 10.69AGTVVTEE166 pKa = 5.19DD167 pKa = 3.86ILTEE171 pKa = 4.62PYY173 pKa = 9.55QLKK176 pKa = 10.39YY177 pKa = 10.42PDD179 pKa = 4.58GEE181 pKa = 4.27TDD183 pKa = 2.91ATAVFDD189 pKa = 4.21GEE191 pKa = 3.99NWTITFGG198 pKa = 3.72

Molecular weight: 21.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A136PQQ5|A0A136PQQ5_9ACTN Metalloenzyme domain-containing protein OS=Micromonospora rosaria OX=47874 GN=AWW66_17295 PE=4 SV=1
MM1 pKa = 7.22AAARR5 pKa = 11.84GRR7 pKa = 11.84RR8 pKa = 11.84GLAGARR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84WRR18 pKa = 11.84RR19 pKa = 11.84TAGLRR24 pKa = 11.84QVPQRR29 pKa = 11.84RR30 pKa = 11.84GGWLGVPRR38 pKa = 11.84GGPAVPGGRR47 pKa = 11.84LPPLRR52 pKa = 11.84QRR54 pKa = 11.84GVQRR58 pKa = 11.84RR59 pKa = 11.84LLRR62 pKa = 11.84AGRR65 pKa = 11.84RR66 pKa = 11.84LLVRR70 pKa = 11.84ILWLAGAGWSEE81 pKa = 4.17GG82 pKa = 3.56

Molecular weight: 9.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5974

0

5974

1999827

29

4602

334.8

35.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.951 ± 0.051

0.742 ± 0.009

6.135 ± 0.025

4.973 ± 0.029

2.594 ± 0.016

9.473 ± 0.029

2.199 ± 0.014

2.931 ± 0.021

1.419 ± 0.022

10.601 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.514 ± 0.012

1.688 ± 0.019

6.504 ± 0.033

2.718 ± 0.018

8.684 ± 0.034

4.45 ± 0.024

6.42 ± 0.032

9.326 ± 0.032

1.591 ± 0.012

2.087 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski