Mola mola (Ocean sunfish) (Tetraodon mola)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28963 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q3W7H1|A0A3Q3W7H1_MOLML Uncharacterized protein OS=Mola mola OX=94237 PE=4 SV=1
MM1 pKa = 7.7EE2 pKa = 5.35DD3 pKa = 4.17AEE5 pKa = 4.52TEE7 pKa = 4.25LAVTEE12 pKa = 4.23PDD14 pKa = 3.18ALGADD19 pKa = 5.39FITVEE24 pKa = 5.27LDD26 pKa = 3.4TQPIEE31 pKa = 4.73YY32 pKa = 8.85VVKK35 pKa = 9.05WAEE38 pKa = 4.01VGSKK42 pKa = 7.6FTISCVKK49 pKa = 10.18KK50 pKa = 10.8DD51 pKa = 3.54SDD53 pKa = 4.69DD54 pKa = 3.84ATEE57 pKa = 4.07LTADD61 pKa = 3.51QLKK64 pKa = 10.39IEE66 pKa = 4.1TDD68 pKa = 3.38EE69 pKa = 5.28AFFAPYY75 pKa = 10.42EE76 pKa = 4.01EE77 pKa = 5.08VYY79 pKa = 10.04PCEE82 pKa = 4.03VTEE85 pKa = 3.93QSVEE89 pKa = 3.85IKK91 pKa = 9.84TDD93 pKa = 3.24SDD95 pKa = 3.34EE96 pKa = 4.82DD97 pKa = 3.9EE98 pKa = 4.61EE99 pKa = 6.22DD100 pKa = 3.84IEE102 pKa = 5.44DD103 pKa = 3.8EE104 pKa = 4.54QEE106 pKa = 4.22VLVEE110 pKa = 4.5AGSSQLDD117 pKa = 3.76GEE119 pKa = 4.57VDD121 pKa = 3.16SDD123 pKa = 3.98TADD126 pKa = 3.58FEE128 pKa = 4.87PDD130 pKa = 2.76EE131 pKa = 4.21RR132 pKa = 11.84QYY134 pKa = 11.39RR135 pKa = 11.84CSYY138 pKa = 10.16CGKK141 pKa = 9.16CYY143 pKa = 10.75SHH145 pKa = 7.04ASSLYY150 pKa = 9.5RR151 pKa = 11.84HH152 pKa = 5.7QQTHH156 pKa = 4.78

Molecular weight: 17.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q4BC77|A0A3Q4BC77_MOLML AKT1 substrate 1 (proline-rich) OS=Mola mola OX=94237 PE=4 SV=1
MM1 pKa = 6.96VQTPTHH7 pKa = 6.38PPIHH11 pKa = 6.64PSIHH15 pKa = 6.47PSIHH19 pKa = 6.42PSIHH23 pKa = 6.42PSIHH27 pKa = 6.42PSIHH31 pKa = 6.42PSIHH35 pKa = 6.42PSIHH39 pKa = 6.42PSIHH43 pKa = 6.42PSIHH47 pKa = 6.61PSILPPRR54 pKa = 11.84PTRR57 pKa = 11.84GSSPPPTLFAGVPLQPQLIWSSSVAVRR84 pKa = 11.84MSVTRR89 pKa = 11.84PVPMVLFPSLKK100 pKa = 9.16VNLWPFSRR108 pKa = 11.84TIGWRR113 pKa = 11.84SVSVKK118 pKa = 10.1VVSSPGITISS128 pKa = 3.25

Molecular weight: 13.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21362

7601

28963

14064223

9

6187

485.6

54.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.451 ± 0.013

2.446 ± 0.015

5.062 ± 0.011

6.493 ± 0.018

3.901 ± 0.011

6.276 ± 0.02

2.704 ± 0.008

4.609 ± 0.011

5.495 ± 0.016

9.831 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.418 ± 0.005

3.89 ± 0.008

5.392 ± 0.017

4.574 ± 0.013

5.625 ± 0.01

8.431 ± 0.017

5.562 ± 0.011

6.527 ± 0.013

1.231 ± 0.005

2.897 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski