Bicyclus anynana (Squinting bush brown butterfly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura;

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19396 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J1N4P6|A0A6J1N4P6_BICAN DNA mismatch repair protein Msh2 OS=Bicyclus anynana OX=110368 GN=LOC112045785 PE=3 SV=1
MM1 pKa = 7.25SVSEE5 pKa = 4.36GEE7 pKa = 4.34GRR9 pKa = 11.84AVWRR13 pKa = 11.84LGEE16 pKa = 4.15QGQHH20 pKa = 4.71TLVVAARR27 pKa = 11.84EE28 pKa = 3.99ALAAPSALALPPPEE42 pKa = 4.56PAPGLILPDD51 pKa = 4.24GSINWGCPCLGGMATGPCGTQFRR74 pKa = 11.84DD75 pKa = 3.29AFSCFHH81 pKa = 6.3YY82 pKa = 10.92SEE84 pKa = 5.43AEE86 pKa = 4.03PKK88 pKa = 10.92GSDD91 pKa = 3.58CYY93 pKa = 11.65DD94 pKa = 3.42KK95 pKa = 10.99FSVMQEE101 pKa = 3.8CMAQYY106 pKa = 9.05PQLYY110 pKa = 10.49AKK112 pKa = 10.69DD113 pKa = 4.5DD114 pKa = 4.64DD115 pKa = 4.91DD116 pKa = 7.2DD117 pKa = 4.2EE118 pKa = 5.47LADD121 pKa = 3.93EE122 pKa = 5.5LGAALQGAADD132 pKa = 4.29ASDD135 pKa = 5.0APDD138 pKa = 4.65APDD141 pKa = 5.17APDD144 pKa = 4.03APPPGRR150 pKa = 4.02

Molecular weight: 15.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J1P5H1|A0A6J1P5H1_BICAN zinc metalloproteinase nas-4-like OS=Bicyclus anynana OX=110368 GN=LOC112056827 PE=4 SV=1
MM1 pKa = 7.44SAHH4 pKa = 6.1KK5 pKa = 9.54TFIIKK10 pKa = 10.27RR11 pKa = 11.84KK12 pKa = 8.77LARR15 pKa = 11.84KK16 pKa = 9.26LKK18 pKa = 8.96QNRR21 pKa = 11.84PIPQWVRR28 pKa = 11.84MRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.47RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.1WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.89LKK50 pKa = 10.55LL51 pKa = 3.39

Molecular weight: 6.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14375

5021

19396

11249408

34

19577

580.0

65.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.091 ± 0.026

1.994 ± 0.027

5.652 ± 0.014

6.732 ± 0.024

3.478 ± 0.014

5.53 ± 0.023

2.521 ± 0.009

5.242 ± 0.017

6.434 ± 0.032

8.881 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.176 ± 0.01

4.832 ± 0.018

5.557 ± 0.025

3.996 ± 0.018

5.73 ± 0.021

7.709 ± 0.022

5.842 ± 0.019

6.349 ± 0.017

1.092 ± 0.007

3.158 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski