Amino acid dipepetide frequency for Bicyclus anynana (Squinting bush brown butterfly)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.511AlaAla: 7.511 ± 0.068	1.376AlaCys: 1.376 ± 0.028	3.563AlaAsp: 3.563 ± 0.025	4.48AlaGlu: 4.48 ± 0.032	2.24AlaPhe: 2.24 ± 0.018	4.259AlaGly: 4.259 ± 0.03	1.87AlaHis: 1.87 ± 0.014	3.192AlaIle: 3.192 ± 0.02	3.746AlaLys: 3.746 ± 0.024	6.837AlaLeu: 6.837 ± 0.044	1.496AlaMet: 1.496 ± 0.014	2.652AlaAsn: 2.652 ± 0.016	4.417AlaPro: 4.417 ± 0.038	2.639AlaGln: 2.639 ± 0.022	4.344AlaArg: 4.344 ± 0.034	5.111AlaSer: 5.111 ± 0.03	3.828AlaThr: 3.828 ± 0.023	4.737AlaVal: 4.737 ± 0.027	0.748AlaTrp: 0.748 ± 0.01	1.858AlaTyr: 1.858 ± 0.016	0.004AlaXaa: 0.004 ± 0.001
Cys	1.444CysAla: 1.444 ± 0.02	0.511CysCys: 0.511 ± 0.008	1.273CysAsp: 1.273 ± 0.019	1.289CysGlu: 1.289 ± 0.019	0.71CysPhe: 0.71 ± 0.011	1.402CysGly: 1.402 ± 0.032	0.479CysHis: 0.479 ± 0.009	0.997CysIle: 0.997 ± 0.026	1.114CysLys: 1.114 ± 0.02	1.704CysLeu: 1.704 ± 0.023	0.375CysMet: 0.375 ± 0.007	0.973CysAsn: 0.973 ± 0.017	0.989CysPro: 0.989 ± 0.027	0.68CysGln: 0.68 ± 0.015	1.16CysArg: 1.16 ± 0.03	1.527CysSer: 1.527 ± 0.029	1.085CysThr: 1.085 ± 0.023	1.422CysVal: 1.422 ± 0.023	0.216CysTrp: 0.216 ± 0.004	0.592CysTyr: 0.592 ± 0.009	0.001CysXaa: 0.001 ± 0.0
Asp	3.646AspAla: 3.646 ± 0.025	1.002AspCys: 1.002 ± 0.018	4.147AspAsp: 4.147 ± 0.038	4.358AspGlu: 4.358 ± 0.026	2.101AspPhe: 2.101 ± 0.017	3.215AspGly: 3.215 ± 0.025	1.171AspHis: 1.171 ± 0.012	3.529AspIle: 3.529 ± 0.023	3.697AspLys: 3.697 ± 0.029	4.746AspLeu: 4.746 ± 0.028	1.271AspMet: 1.271 ± 0.011	2.863AspAsn: 2.863 ± 0.023	2.545AspPro: 2.545 ± 0.027	1.627AspGln: 1.627 ± 0.013	2.752AspArg: 2.752 ± 0.026	4.345AspSer: 4.345 ± 0.031	3.203AspThr: 3.203 ± 0.023	3.796AspVal: 3.796 ± 0.021	0.629AspTrp: 0.629 ± 0.011	1.877AspTyr: 1.877 ± 0.015	0.001AspXaa: 0.001 ± 0.0
Glu	4.47GluAla: 4.47 ± 0.032	1.251GluCys: 1.251 ± 0.034	4.063GluAsp: 4.063 ± 0.026	5.964GluGlu: 5.964 ± 0.056	2.062GluPhe: 2.062 ± 0.017	3.213GluGly: 3.213 ± 0.029	1.524GluHis: 1.524 ± 0.012	3.727GluIle: 3.727 ± 0.026	5.109GluLys: 5.109 ± 0.044	5.935GluLeu: 5.935 ± 0.035	1.53GluMet: 1.53 ± 0.013	3.565GluAsn: 3.565 ± 0.027	3.169GluPro: 3.169 ± 0.038	2.693GluGln: 2.693 ± 0.027	4.142GluArg: 4.142 ± 0.031	4.497GluSer: 4.497 ± 0.028	3.666GluThr: 3.666 ± 0.027	4.029GluVal: 4.029 ± 0.029	0.692GluTrp: 0.692 ± 0.008	2.015GluTyr: 2.015 ± 0.017	0.001GluXaa: 0.001 ± 0.0
Phe	2.18PheAla: 2.18 ± 0.017	0.753PheCys: 0.753 ± 0.009	2.055PheAsp: 2.055 ± 0.016	2.009PheGlu: 2.009 ± 0.017	1.32PhePhe: 1.32 ± 0.014	2.226PheGly: 2.226 ± 0.02	0.882PheHis: 0.882 ± 0.009	2.014PheIle: 2.014 ± 0.018	2.084PheLys: 2.084 ± 0.017	3.127PheLeu: 3.127 ± 0.022	0.803PheMet: 0.803 ± 0.01	1.754PheAsn: 1.754 ± 0.013	1.521PhePro: 1.521 ± 0.014	1.277PheGln: 1.277 ± 0.011	1.888PheArg: 1.888 ± 0.02	2.675PheSer: 2.675 ± 0.018	2.146PheThr: 2.146 ± 0.018	2.378PheVal: 2.378 ± 0.018	0.419PheTrp: 0.419 ± 0.007	1.267PheTyr: 1.267 ± 0.012	0.001PheXaa: 0.001 ± 0.0
Gly	4.34GlyAla: 4.34 ± 0.033	1.015GlyCys: 1.015 ± 0.014	3.065GlyAsp: 3.065 ± 0.024	3.485GlyGlu: 3.485 ± 0.03	2.069GlyPhe: 2.069 ± 0.018	4.527GlyGly: 4.527 ± 0.069	1.357GlyHis: 1.357 ± 0.014	2.687GlyIle: 2.687 ± 0.022	3.205GlyLys: 3.205 ± 0.027	4.468GlyLeu: 4.468 ± 0.023	1.078GlyMet: 1.078 ± 0.012	2.412GlyAsn: 2.412 ± 0.018	2.551GlyPro: 2.551 ± 0.042	2.049GlyGln: 2.049 ± 0.033	3.203GlyArg: 3.203 ± 0.023	4.421GlySer: 4.421 ± 0.039	2.968GlyThr: 2.968 ± 0.022	3.686GlyVal: 3.686 ± 0.025	0.706GlyTrp: 0.706 ± 0.009	2.011GlyTyr: 2.011 ± 0.022	0.002GlyXaa: 0.002 ± 0.001
His	1.7HisAla: 1.7 ± 0.017	0.576HisCys: 0.576 ± 0.01	1.195HisAsp: 1.195 ± 0.011	1.39HisGlu: 1.39 ± 0.012	0.955HisPhe: 0.955 ± 0.011	1.332HisGly: 1.332 ± 0.014	0.947HisHis: 0.947 ± 0.017	1.374HisIle: 1.374 ± 0.013	1.467HisLys: 1.467 ± 0.014	2.284HisLeu: 2.284 ± 0.017	0.747HisMet: 0.747 ± 0.012	1.187HisAsn: 1.187 ± 0.013	1.352HisPro: 1.352 ± 0.011	1.015HisGln: 1.015 ± 0.012	1.453HisArg: 1.453 ± 0.013	1.928HisSer: 1.928 ± 0.014	1.577HisThr: 1.577 ± 0.018	1.551HisVal: 1.551 ± 0.012	0.295HisTrp: 0.295 ± 0.005	0.888HisTyr: 0.888 ± 0.009	0.001HisXaa: 0.001 ± 0.0
Ile	3.332IleAla: 3.332 ± 0.021	1.171IleCys: 1.171 ± 0.022	3.157IleAsp: 3.157 ± 0.025	3.51IleGlu: 3.51 ± 0.026	1.989IlePhe: 1.989 ± 0.018	2.561IleGly: 2.561 ± 0.018	1.271IleHis: 1.271 ± 0.013	3.105IleIle: 3.105 ± 0.021	3.752IleLys: 3.752 ± 0.029	4.647IleLeu: 4.647 ± 0.033	1.112IleMet: 1.112 ± 0.013	2.806IleAsn: 2.806 ± 0.025	2.665IlePro: 2.665 ± 0.02	2.065IleGln: 2.065 ± 0.015	2.506IleArg: 2.506 ± 0.017	3.937IleSer: 3.937 ± 0.024	3.3IleThr: 3.3 ± 0.023	3.417IleVal: 3.417 ± 0.02	0.499IleTrp: 0.499 ± 0.008	1.62IleTyr: 1.62 ± 0.012	0.002IleXaa: 0.002 ± 0.0
Lys	3.635LysAla: 3.635 ± 0.028	1.294LysCys: 1.294 ± 0.023	3.644LysAsp: 3.644 ± 0.029	4.877LysGlu: 4.877 ± 0.043	2.081LysPhe: 2.081 ± 0.015	2.676LysGly: 2.676 ± 0.023	1.614LysHis: 1.614 ± 0.015	3.661LysIle: 3.661 ± 0.025	5.486LysLys: 5.486 ± 0.056	5.659LysLeu: 5.659 ± 0.035	1.484LysMet: 1.484 ± 0.011	3.274LysAsn: 3.274 ± 0.025	3.608LysPro: 3.608 ± 0.048	2.568LysGln: 2.568 ± 0.024	3.713LysArg: 3.713 ± 0.027	4.769LysSer: 4.769 ± 0.036	3.69LysThr: 3.69 ± 0.03	3.728LysVal: 3.728 ± 0.022	0.664LysTrp: 0.664 ± 0.013	2.212LysTyr: 2.212 ± 0.019	0.002LysXaa: 0.002 ± 0.0
Leu	6.528LeuAla: 6.528 ± 0.039	1.876LeuCys: 1.876 ± 0.021	4.829LeuAsp: 4.829 ± 0.028	5.937LeuGlu: 5.937 ± 0.037	2.979LeuPhe: 2.979 ± 0.025	4.372LeuGly: 4.372 ± 0.029	2.346LeuHis: 2.346 ± 0.018	4.126LeuIle: 4.126 ± 0.027	5.882LeuLys: 5.882 ± 0.028	8.611LeuLeu: 8.611 ± 0.057	1.9LeuMet: 1.9 ± 0.016	4.156LeuAsn: 4.156 ± 0.026	4.771LeuPro: 4.771 ± 0.026	4.308LeuGln: 4.308 ± 0.027	5.515LeuArg: 5.515 ± 0.029	6.831LeuSer: 6.831 ± 0.033	4.929LeuThr: 4.929 ± 0.021	5.323LeuVal: 5.323 ± 0.026	0.918LeuTrp: 0.918 ± 0.013	2.666LeuTyr: 2.666 ± 0.018	0.003LeuXaa: 0.003 ± 0.0
Met	1.564MetAla: 1.564 ± 0.014	0.436MetCys: 0.436 ± 0.009	1.226MetAsp: 1.226 ± 0.01	1.525MetGlu: 1.525 ± 0.015	0.876MetPhe: 0.876 ± 0.01	1.143MetGly: 1.143 ± 0.015	0.542MetHis: 0.542 ± 0.008	1.0MetIle: 1.0 ± 0.01	1.417MetLys: 1.417 ± 0.013	1.931MetLeu: 1.931 ± 0.019	0.602MetMet: 0.602 ± 0.008	0.994MetAsn: 0.994 ± 0.01	1.118MetPro: 1.118 ± 0.012	0.934MetGln: 0.934 ± 0.011	1.251MetArg: 1.251 ± 0.013	1.798MetSer: 1.798 ± 0.013	1.208MetThr: 1.208 ± 0.011	1.25MetVal: 1.25 ± 0.013	0.25MetTrp: 0.25 ± 0.005	0.7MetTyr: 0.7 ± 0.009	0.001MetXaa: 0.001 ± 0.0
Asn	2.825AsnAla: 2.825 ± 0.02	0.879AsnCys: 0.879 ± 0.014	2.646AsnAsp: 2.646 ± 0.021	3.169AsnGlu: 3.169 ± 0.022	1.781AsnPhe: 1.781 ± 0.016	2.803AsnGly: 2.803 ± 0.019	1.074AsnHis: 1.074 ± 0.011	3.305AsnIle: 3.305 ± 0.026	3.359AsnLys: 3.359 ± 0.028	4.169AsnLeu: 4.169 ± 0.027	1.202AsnMet: 1.202 ± 0.012	3.063AsnAsn: 3.063 ± 0.026	2.147AsnPro: 2.147 ± 0.025	1.789AsnGln: 1.789 ± 0.019	2.22AsnArg: 2.22 ± 0.018	3.633AsnSer: 3.633 ± 0.028	2.852AsnThr: 2.852 ± 0.019	3.234AsnVal: 3.234 ± 0.022	0.476AsnTrp: 0.476 ± 0.007	1.69AsnTyr: 1.69 ± 0.015	0.001AsnXaa: 0.001 ± 0.0
Pro	4.393ProAla: 4.393 ± 0.032	0.794ProCys: 0.794 ± 0.039	2.962ProAsp: 2.962 ± 0.019	3.712ProGlu: 3.712 ± 0.033	1.667ProPhe: 1.667 ± 0.018	3.053ProGly: 3.053 ± 0.06	1.474ProHis: 1.474 ± 0.014	2.505ProIle: 2.505 ± 0.022	3.168ProLys: 3.168 ± 0.026	4.372ProLeu: 4.372 ± 0.027	0.981ProMet: 0.981 ± 0.012	2.324ProAsn: 2.324 ± 0.022	5.32ProPro: 5.32 ± 0.075	2.308ProGln: 2.308 ± 0.021	3.073ProArg: 3.073 ± 0.028	4.375ProSer: 4.375 ± 0.057	3.314ProThr: 3.314 ± 0.034	3.557ProVal: 3.557 ± 0.028	0.511ProTrp: 0.511 ± 0.007	1.702ProTyr: 1.702 ± 0.017	0.003ProXaa: 0.003 ± 0.001
Gln	2.595GlnAla: 2.595 ± 0.022	0.805GlnCys: 0.805 ± 0.016	1.849GlnAsp: 1.849 ± 0.014	2.59GlnGlu: 2.59 ± 0.021	1.356GlnPhe: 1.356 ± 0.011	1.834GlnGly: 1.834 ± 0.021	1.171GlnHis: 1.171 ± 0.012	2.079GlnIle: 2.079 ± 0.016	2.447GlnLys: 2.447 ± 0.02	3.789GlnLeu: 3.789 ± 0.027	0.963GlnMet: 0.963 ± 0.013	2.164GlnAsn: 2.164 ± 0.022	2.32GlnPro: 2.32 ± 0.03	2.509GlnGln: 2.509 ± 0.053	2.473GlnArg: 2.473 ± 0.02	2.81GlnSer: 2.81 ± 0.023	2.186GlnThr: 2.186 ± 0.018	2.202GlnVal: 2.202 ± 0.018	0.476GlnTrp: 0.476 ± 0.007	1.337GlnTyr: 1.337 ± 0.014	0.001GlnXaa: 0.001 ± 0.0
Arg	4.432ArgAla: 4.432 ± 0.032	1.152ArgCys: 1.152 ± 0.023	3.206ArgAsp: 3.206 ± 0.025	3.565ArgGlu: 3.565 ± 0.024	1.892ArgPhe: 1.892 ± 0.013	3.129ArgGly: 3.129 ± 0.026	1.638ArgHis: 1.638 ± 0.014	2.634ArgIle: 2.634 ± 0.02	3.602ArgLys: 3.602 ± 0.026	5.158ArgLeu: 5.158 ± 0.031	1.13ArgMet: 1.13 ± 0.01	2.581ArgAsn: 2.581 ± 0.017	3.114ArgPro: 3.114 ± 0.033	2.242ArgGln: 2.242 ± 0.02	4.784ArgArg: 4.784 ± 0.037	4.305ArgSer: 4.305 ± 0.034	2.97ArgThr: 2.97 ± 0.017	3.376ArgVal: 3.376 ± 0.026	0.693ArgTrp: 0.693 ± 0.01	1.694ArgTyr: 1.694 ± 0.015	0.003ArgXaa: 0.003 ± 0.001
Ser	5.248SerAla: 5.248 ± 0.031	1.481SerCys: 1.481 ± 0.03	4.529SerAsp: 4.529 ± 0.027	4.959SerGlu: 4.959 ± 0.033	2.563SerPhe: 2.563 ± 0.018	4.602SerGly: 4.602 ± 0.041	1.733SerHis: 1.733 ± 0.015	3.788SerIle: 3.788 ± 0.023	4.578SerLys: 4.578 ± 0.031	6.62SerLeu: 6.62 ± 0.032	1.607SerMet: 1.607 ± 0.016	3.65SerAsn: 3.65 ± 0.026	4.807SerPro: 4.807 ± 0.066	2.933SerGln: 2.933 ± 0.022	4.039SerArg: 4.039 ± 0.033	7.415SerSer: 7.415 ± 0.058	4.767SerThr: 4.767 ± 0.033	4.767SerVal: 4.767 ± 0.023	0.816SerTrp: 0.816 ± 0.01	2.186SerTyr: 2.186 ± 0.016	0.003SerXaa: 0.003 ± 0.001
Thr	3.944ThrAla: 3.944 ± 0.025	1.134ThrCys: 1.134 ± 0.02	3.152ThrAsp: 3.152 ± 0.023	3.784ThrGlu: 3.784 ± 0.029	2.111ThrPhe: 2.111 ± 0.015	3.298ThrGly: 3.298 ± 0.028	1.464ThrHis: 1.464 ± 0.017	3.099ThrIle: 3.099 ± 0.02	3.492ThrLys: 3.492 ± 0.03	5.181ThrLeu: 5.181 ± 0.028	1.145ThrMet: 1.145 ± 0.012	2.741ThrAsn: 2.741 ± 0.021	3.764ThrPro: 3.764 ± 0.029	2.159ThrGln: 2.159 ± 0.02	2.718ThrArg: 2.718 ± 0.019	4.754ThrSer: 4.754 ± 0.035	4.156ThrThr: 4.156 ± 0.078	3.998ThrVal: 3.998 ± 0.024	0.613ThrTrp: 0.613 ± 0.01	1.713ThrTyr: 1.713 ± 0.014	0.002ThrXaa: 0.002 ± 0.0
Val	4.558ValAla: 4.558 ± 0.028	1.485ValCys: 1.485 ± 0.022	3.508ValAsp: 3.508 ± 0.02	4.076ValGlu: 4.076 ± 0.034	2.3ValPhe: 2.3 ± 0.017	3.176ValGly: 3.176 ± 0.021	1.549ValHis: 1.549 ± 0.014	3.359ValIle: 3.359 ± 0.023	4.019ValLys: 4.019 ± 0.027	5.698ValLeu: 5.698 ± 0.032	1.357ValMet: 1.357 ± 0.012	2.994ValAsn: 2.994 ± 0.022	3.489ValPro: 3.489 ± 0.028	2.468ValGln: 2.468 ± 0.017	3.465ValArg: 3.465 ± 0.024	4.751ValSer: 4.751 ± 0.021	4.14ValThr: 4.14 ± 0.033	4.424ValVal: 4.424 ± 0.026	0.705ValTrp: 0.705 ± 0.009	1.964ValTyr: 1.964 ± 0.017	0.002ValXaa: 0.002 ± 0.0
Trp	0.667TrpAla: 0.667 ± 0.009	0.228TrpCys: 0.228 ± 0.004	0.609TrpAsp: 0.609 ± 0.009	0.65TrpGlu: 0.65 ± 0.01	0.435TrpPhe: 0.435 ± 0.007	0.58TrpGly: 0.58 ± 0.009	0.26TrpHis: 0.26 ± 0.005	0.546TrpIle: 0.546 ± 0.009	0.661TrpLys: 0.661 ± 0.01	1.134TrpLeu: 1.134 ± 0.013	0.274TrpMet: 0.274 ± 0.006	0.493TrpAsn: 0.493 ± 0.007	0.454TrpPro: 0.454 ± 0.007	0.463TrpGln: 0.463 ± 0.007	0.815TrpArg: 0.815 ± 0.013	0.841TrpSer: 0.841 ± 0.01	0.601TrpThr: 0.601 ± 0.009	0.636TrpVal: 0.636 ± 0.011	0.207TrpTrp: 0.207 ± 0.005	0.36TrpTyr: 0.36 ± 0.008	0.001TrpXaa: 0.001 ± 0.0
Tyr	1.893TyrAla: 1.893 ± 0.015	0.726TyrCys: 0.726 ± 0.01	1.842TyrAsp: 1.842 ± 0.015	1.985TyrGlu: 1.985 ± 0.016	1.291TyrPhe: 1.291 ± 0.013	1.899TyrGly: 1.899 ± 0.017	0.844TyrHis: 0.844 ± 0.009	1.693TyrIle: 1.693 ± 0.016	2.051TyrLys: 2.051 ± 0.019	2.736TyrLeu: 2.736 ± 0.018	0.71TyrMet: 0.71 ± 0.009	1.672TyrAsn: 1.672 ± 0.016	1.444TyrPro: 1.444 ± 0.016	1.229TyrGln: 1.229 ± 0.013	1.784TyrArg: 1.784 ± 0.014	2.366TyrSer: 2.366 ± 0.019	1.833TyrThr: 1.833 ± 0.015	1.976TyrVal: 1.976 ± 0.016	0.38TyrTrp: 0.38 ± 0.006	1.23TyrTyr: 1.23 ± 0.013	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.004XaaAla: 0.004 ± 0.001	0.001XaaCys: 0.001 ± 0.0	0.002XaaAsp: 0.002 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.003XaaGly: 0.003 ± 0.001	0.001XaaHis: 0.001 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.002XaaLys: 0.002 ± 0.0	0.003XaaLeu: 0.003 ± 0.0	0.001XaaMet: 0.001 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.002XaaPro: 0.002 ± 0.0	0.002XaaGln: 0.002 ± 0.0	0.003XaaArg: 0.003 ± 0.001	0.002XaaSer: 0.002 ± 0.001	0.002XaaThr: 0.002 ± 0.0	0.002XaaVal: 0.002 ± 0.0	0.001XaaTrp: 0.001 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.007XaaXaa: 0.007 ± 0.003

Statistics based on 19396 proteins (11249408 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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