Calothrix sp. NIES-3974

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Calotrichaceae; Calothrix; unclassified Calothrix

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4751 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z4NDX5|A0A1Z4NDX5_9CYAN Glucose 1-dehydrogenase OS=Calothrix sp. NIES-3974 OX=2005462 GN=NIES3974_05500 PE=4 SV=1
MM1 pKa = 7.04QLSATEE7 pKa = 4.01AQQIALEE14 pKa = 4.39FLLQDD19 pKa = 3.43WNIPDD24 pKa = 5.67AISDD28 pKa = 3.62WFVVISSRR36 pKa = 11.84LMGQYY41 pKa = 9.5WYY43 pKa = 9.92IVEE46 pKa = 4.23IGIAGLPDD54 pKa = 2.62RR55 pKa = 11.84WYY57 pKa = 10.03IQVYY61 pKa = 8.31DD62 pKa = 4.17TGEE65 pKa = 4.16CDD67 pKa = 3.25PNYY70 pKa = 10.3TFVSPIKK77 pKa = 9.86STEE80 pKa = 3.46IDD82 pKa = 3.21TDD84 pKa = 3.63LGEE87 pKa = 4.56FPAIIAEE94 pKa = 4.31VLSSEE99 pKa = 4.3RR100 pKa = 11.84NSRR103 pKa = 3.44

Molecular weight: 11.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z4NH93|A0A1Z4NH93_9CYAN Uncharacterized protein OS=Calothrix sp. NIES-3974 OX=2005462 GN=NIES3974_17330 PE=4 SV=1
MM1 pKa = 6.53TTVTSTKK8 pKa = 10.3ASSLLGISDD17 pKa = 3.39SRR19 pKa = 11.84VRR21 pKa = 11.84QLLGEE26 pKa = 4.03GRR28 pKa = 11.84IDD30 pKa = 3.46GAYY33 pKa = 9.83KK34 pKa = 10.45EE35 pKa = 4.59GRR37 pKa = 11.84CWRR40 pKa = 11.84IPLFKK45 pKa = 10.68GMPRR49 pKa = 11.84IIAGKK54 pKa = 9.75RR55 pKa = 11.84GPKK58 pKa = 9.14GHH60 pKa = 5.68WRR62 pKa = 11.84MRR64 pKa = 11.84IQKK67 pKa = 8.71VRR69 pKa = 11.84TYY71 pKa = 10.5IHH73 pKa = 6.21VNKK76 pKa = 10.0HH77 pKa = 4.16QIARR81 pKa = 11.84NRR83 pKa = 11.84RR84 pKa = 11.84EE85 pKa = 4.14NQQHH89 pKa = 5.43PVIVVRR95 pKa = 11.84AGKK98 pKa = 10.01GRR100 pKa = 11.84PKK102 pKa = 10.19YY103 pKa = 10.07CNEE106 pKa = 3.45VEE108 pKa = 4.08IRR110 pKa = 11.84GSCRR114 pKa = 11.84LVYY117 pKa = 10.34RR118 pKa = 11.84PEE120 pKa = 3.94QPLGCSGAVLWIEE133 pKa = 3.98VEE135 pKa = 4.13PEE137 pKa = 3.72VEE139 pKa = 3.8IVTKK143 pKa = 10.55KK144 pKa = 9.92RR145 pKa = 11.84CQVYY149 pKa = 10.12

Molecular weight: 17.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4751

0

4751

1469257

29

2198

309.3

34.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.805 ± 0.032

0.999 ± 0.013

4.782 ± 0.026

5.939 ± 0.037

4.013 ± 0.027

6.755 ± 0.038

1.984 ± 0.02

7.256 ± 0.03

4.471 ± 0.03

10.562 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.977 ± 0.017

4.632 ± 0.038

4.877 ± 0.029

5.543 ± 0.034

5.339 ± 0.027

6.167 ± 0.031

5.764 ± 0.029

6.608 ± 0.035

1.429 ± 0.017

3.099 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski