Caenispirillum bisanense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Caenispirillum

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4942 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286H2A1|A0A286H2A1_9PROT Transposase OS=Caenispirillum bisanense OX=414052 GN=SAMN05421508_1302 PE=4 SV=1
MM1 pKa = 8.04PKK3 pKa = 9.75PQPTSTRR10 pKa = 11.84TPLFDD15 pKa = 3.5TDD17 pKa = 3.96GFTSALVVDD26 pKa = 4.7AALASEE32 pKa = 4.4ISYY35 pKa = 10.93GRR37 pKa = 11.84GDD39 pKa = 4.33LDD41 pKa = 3.74AALDD45 pKa = 3.84AGGFTNVTADD55 pKa = 2.71IGLPAEE61 pKa = 4.08YY62 pKa = 9.78LVNGFFVNGNASGIVLEE79 pKa = 4.55KK80 pKa = 11.14DD81 pKa = 3.06GVLYY85 pKa = 10.81VAFAGTSVEE94 pKa = 4.34NPLPDD99 pKa = 4.39VLDD102 pKa = 3.53YY103 pKa = 10.5WQLNGQAMIDD113 pKa = 3.79EE114 pKa = 4.86YY115 pKa = 11.44YY116 pKa = 11.19GDD118 pKa = 3.78VSAMMDD124 pKa = 3.06AAMTYY129 pKa = 10.65AADD132 pKa = 4.09PEE134 pKa = 4.71SGVTQVVATGHH145 pKa = 5.89SLGGSAVDD153 pKa = 4.06GALAAYY159 pKa = 9.34DD160 pKa = 3.58AVEE163 pKa = 4.11YY164 pKa = 9.16PALRR168 pKa = 11.84GITFNSPTSGLLDD181 pKa = 4.41DD182 pKa = 5.02GRR184 pKa = 11.84EE185 pKa = 4.08LNVGYY190 pKa = 10.62EE191 pKa = 3.79NDD193 pKa = 4.7FINEE197 pKa = 4.15IIGDD201 pKa = 4.09GDD203 pKa = 3.67AGNAVHH209 pKa = 6.53NLYY212 pKa = 10.68YY213 pKa = 10.44AIQPGEE219 pKa = 4.13PMPDD223 pKa = 3.41PGSEE227 pKa = 3.68TSYY230 pKa = 10.98IALLSKK236 pKa = 10.55ALQAWTSPAHH246 pKa = 6.81DD247 pKa = 3.11IARR250 pKa = 11.84FPEE253 pKa = 4.17ALAALTEE260 pKa = 4.42SPVYY264 pKa = 9.77EE265 pKa = 4.03QVSRR269 pKa = 11.84GDD271 pKa = 3.56VVVLDD276 pKa = 3.95GSRR279 pKa = 11.84YY280 pKa = 9.35GVSLDD285 pKa = 3.84AMADD289 pKa = 3.75LSPAFDD295 pKa = 3.45AAMAGSSRR303 pKa = 11.84VGLVGQAVDD312 pKa = 4.09DD313 pKa = 4.17VLGGSAYY320 pKa = 10.53GDD322 pKa = 4.64AIDD325 pKa = 5.5GGAGDD330 pKa = 5.25DD331 pKa = 4.24VIAGLGGNDD340 pKa = 4.3LLWGGEE346 pKa = 4.08GLDD349 pKa = 3.42TFLFTSRR356 pKa = 11.84FGTDD360 pKa = 2.79VVKK363 pKa = 10.53DD364 pKa = 3.82YY365 pKa = 11.14EE366 pKa = 5.35AGDD369 pKa = 3.41ILSFQTGPAGGEE381 pKa = 4.05YY382 pKa = 10.24LASLAALQAWTEE394 pKa = 4.13STNQIRR400 pKa = 11.84TSFTDD405 pKa = 2.96AGLVIAFAGGKK416 pKa = 10.1DD417 pKa = 3.27KK418 pKa = 10.85VTLEE422 pKa = 4.51GVGSWEE428 pKa = 4.46LMAA431 pKa = 6.62

Molecular weight: 44.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286H0I6|A0A286H0I6_9PROT TRAP-type mannitol/chloroaromatic compound transport system small permease component OS=Caenispirillum bisanense OX=414052 GN=SAMN05421508_11450 PE=4 SV=1
MM1 pKa = 7.06NLWLRR6 pKa = 11.84LLRR9 pKa = 11.84IIVGALLGRR18 pKa = 11.84RR19 pKa = 11.84LGMFDD24 pKa = 3.02TSEE27 pKa = 3.6LRR29 pKa = 11.84FRR31 pKa = 11.84VWPHH35 pKa = 6.72DD36 pKa = 3.91LDD38 pKa = 5.79LNIHH42 pKa = 5.81MNNARR47 pKa = 11.84YY48 pKa = 9.47LSLMDD53 pKa = 5.06LGRR56 pKa = 11.84ADD58 pKa = 4.95LIIRR62 pKa = 11.84TGLHH66 pKa = 4.22TVMLRR71 pKa = 11.84EE72 pKa = 4.22GWQAVIGGTTVRR84 pKa = 11.84FRR86 pKa = 11.84RR87 pKa = 11.84PLKK90 pKa = 10.14PFQACRR96 pKa = 11.84LTTRR100 pKa = 11.84LLCWDD105 pKa = 4.5DD106 pKa = 2.71RR107 pKa = 11.84WLYY110 pKa = 10.06IEE112 pKa = 5.32HH113 pKa = 7.73RR114 pKa = 11.84ITTADD119 pKa = 3.26GTLACAALVKK129 pKa = 10.61AVFIRR134 pKa = 11.84KK135 pKa = 9.24GSRR138 pKa = 11.84VAPAEE143 pKa = 3.94TVARR147 pKa = 11.84IGVTTPSPAAPDD159 pKa = 3.5WVKK162 pKa = 10.57VPEE165 pKa = 4.32VVPEE169 pKa = 3.88

Molecular weight: 19.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4942

0

4942

1577073

24

4564

319.1

34.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.921 ± 0.059

0.862 ± 0.012

6.085 ± 0.03

5.73 ± 0.033

3.314 ± 0.021

8.832 ± 0.033

2.041 ± 0.017

4.035 ± 0.025

2.677 ± 0.027

10.436 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.411 ± 0.018

1.993 ± 0.018

5.599 ± 0.03

2.86 ± 0.019

7.663 ± 0.046

4.282 ± 0.024

5.493 ± 0.035

8.355 ± 0.03

1.371 ± 0.014

2.04 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski