Herrania umbratica

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales; Malvaceae; Byttnerioideae; Herrania

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23848 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J1BQI0|A0A6J1BQI0_9ROSI uncharacterized protein LOC110428928 OS=Herrania umbratica OX=108875 GN=LOC110428928 PE=3 SV=1
HHH2 pKa = 7.07CSTPLLCSAVNNLPMGYYY20 pKa = 8.92VLQQPPIPATGQPHHH35 pKa = 7.19DDD37 pKa = 3.05MGISNCHHH45 pKa = 4.94VNGVPAPSNFQPMRR59 pKa = 11.84MNSGNDDD66 pKa = 2.79VMEEE70 pKa = 4.29NATNVTPAVPPTTTMSSVSEEE91 pKa = 4.19PVSPTSVASSGNFPFTASDDD111 pKa = 3.55SGMGVDDD118 pKa = 4.24SALDDD123 pKa = 3.47AFTTDDD129 pKa = 3.02ASSVGLQLGPDDD141 pKa = 3.28GAGNSRR147 pKa = 11.84DDD149 pKa = 3.58LRR151 pKa = 11.84SLDDD155 pKa = 3.67IQWNFSLTDDD165 pKa = 3.54TADDD169 pKa = 4.51SNLGDDD175 pKa = 4.41GALGNYYY182 pKa = 9.32GSPFLPSDDD191 pKa = 3.82EEE193 pKa = 4.11LLDDD197 pKa = 4.49PEEE200 pKa = 3.97EEE202 pKa = 4.86DD203 pKa = 3.6VEEE206 pKa = 4.18EE207 pKa = 4.25FVDDD211 pKa = 3.95VPGQPCSQSDDD222 pKa = 3.83EE223 pKa = 4.43EE224 pKa = 4.69KK225 pKa = 10.95

Molecular weight: 23.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J0ZYK4|A0A6J0ZYK4_9ROSI uncharacterized protein LOC110413221 OS=Herrania umbratica OX=108875 GN=LOC110413221 PE=4 SV=1
MM1 pKa = 8.15RR2 pKa = 11.84DD3 pKa = 2.98PGARR7 pKa = 11.84RR8 pKa = 11.84GSSPAWAAGGEE19 pKa = 4.37LGRR22 pKa = 11.84APARR26 pKa = 11.84LAAARR31 pKa = 11.84AGAARR36 pKa = 11.84RR37 pKa = 11.84ARR39 pKa = 11.84PPRR42 pKa = 11.84PRR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84APPRR50 pKa = 11.84GARR53 pKa = 11.84RR54 pKa = 11.84PEE56 pKa = 4.24GVVGAAPGAPGAARR70 pKa = 11.84RR71 pKa = 11.84AAPGPAAAGRR81 pKa = 11.84PGAAARR87 pKa = 11.84RR88 pKa = 11.84PPRR91 pKa = 11.84AAPARR96 pKa = 11.84SAGAGGAAAGGRR108 pKa = 11.84GRR110 pKa = 11.84RR111 pKa = 11.84AAAAPGRR118 pKa = 11.84GRR120 pKa = 11.84RR121 pKa = 11.84GGAAGAGGGPGRR133 pKa = 11.84ARR135 pKa = 11.84RR136 pKa = 11.84ARR138 pKa = 11.84AAAPGRR144 pKa = 11.84PPRR147 pKa = 11.84ACGAARR153 pKa = 11.84PGSRR157 pKa = 11.84LGAAAGPTSAGRR169 pKa = 11.84RR170 pKa = 11.84RR171 pKa = 11.84GAGGRR176 pKa = 11.84RR177 pKa = 11.84RR178 pKa = 11.84RR179 pKa = 11.84GSAPGRR185 pKa = 11.84RR186 pKa = 11.84AAGGGGGAGSGARR199 pKa = 11.84ARR201 pKa = 11.84RR202 pKa = 11.84PGRR205 pKa = 11.84GPAAPACARR214 pKa = 11.84RR215 pKa = 11.84CPAGAAGRR223 pKa = 11.84LLINLL228 pKa = 4.34

Molecular weight: 21.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18898

4950

23848

11398064

37

5457

477.9

53.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.819 ± 0.012

1.909 ± 0.007

5.237 ± 0.01

6.524 ± 0.016

4.229 ± 0.009

6.492 ± 0.012

2.349 ± 0.006

5.282 ± 0.009

6.106 ± 0.015

9.921 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.402 ± 0.006

4.436 ± 0.01

4.857 ± 0.013

3.798 ± 0.012

5.264 ± 0.011

9.06 ± 0.017

4.752 ± 0.008

6.478 ± 0.01

1.275 ± 0.005

2.771 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski