Enterococcus phage phiEf11

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C9E2J2|C9E2J2_9CAUD Putative phage protein OS=Enterococcus phage phiEf11 OX=663241 GN=PHIEF11_0007 PE=4 SV=1
MM1 pKa = 7.28TEE3 pKa = 4.75RR4 pKa = 11.84RR5 pKa = 11.84DD6 pKa = 3.81SMNDD10 pKa = 3.18DD11 pKa = 3.96PYY13 pKa = 11.67DD14 pKa = 3.96YY15 pKa = 11.4LDD17 pKa = 4.26ADD19 pKa = 3.66YY20 pKa = 11.47EE21 pKa = 4.29EE22 pKa = 4.53YY23 pKa = 10.66LRR25 pKa = 11.84KK26 pKa = 10.28EE27 pKa = 4.31EE28 pKa = 4.27VNEE31 pKa = 4.21STKK34 pKa = 9.7EE35 pKa = 4.1TSSDD39 pKa = 3.09

Molecular weight: 4.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C9E2J9|C9E2J9_9CAUD Putative major structural protein OS=Enterococcus phage phiEf11 OX=663241 GN=PHIEF11_0014 PE=4 SV=1
MM1 pKa = 7.86AISQVRR7 pKa = 11.84INGLAGISKK16 pKa = 10.06KK17 pKa = 10.56LKK19 pKa = 10.57RR20 pKa = 11.84NAQLDD25 pKa = 3.93DD26 pKa = 3.58VKK28 pKa = 11.14KK29 pKa = 10.31VVRR32 pKa = 11.84NNTAEE37 pKa = 4.08LTANMQAEE45 pKa = 4.34AGKK48 pKa = 10.42VLTGHH53 pKa = 6.95RR54 pKa = 11.84EE55 pKa = 3.64GKK57 pKa = 10.45KK58 pKa = 9.59FVKK61 pKa = 8.9PTGATKK67 pKa = 10.42RR68 pKa = 11.84SIVMRR73 pKa = 11.84LSNNGFSGHH82 pKa = 6.26TGPGTEE88 pKa = 3.92YY89 pKa = 10.97APYY92 pKa = 9.6LIHH95 pKa = 6.37GTRR98 pKa = 11.84FMVKK102 pKa = 9.1RR103 pKa = 11.84DD104 pKa = 3.69FFLPPLKK111 pKa = 9.69QQKK114 pKa = 9.72VKK116 pKa = 10.51FRR118 pKa = 11.84TDD120 pKa = 3.28LEE122 pKa = 4.32RR123 pKa = 11.84LMKK126 pKa = 10.6

Molecular weight: 14.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

13236

39

1135

203.6

23.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.293 ± 0.376

0.574 ± 0.099

5.848 ± 0.285

7.706 ± 0.375

3.982 ± 0.235

5.878 ± 0.308

1.36 ± 0.193

7.079 ± 0.248

9.134 ± 0.459

8.28 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.478 ± 0.169

6.331 ± 0.231

3.037 ± 0.183

4.072 ± 0.264

3.634 ± 0.22

6.671 ± 0.275

6.324 ± 0.305

6.316 ± 0.222

1.08 ± 0.088

3.921 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski