Nematocida sp. 1 (strain ERTm2 / ATCC PRA-371) (Nematode killer fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Microsporidia incertae sedis; Nematocida; unclassified Nematocida; Nematocida sp. 1

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2769 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H8ZCG3|H8ZCG3_NEMS1 Uncharacterized protein OS=Nematocida sp. 1 (strain ERTm2 / ATCC PRA-371) OX=944018 GN=NERG_01406 PE=4 SV=1
MM1 pKa = 7.87CYY3 pKa = 9.58FAGHH7 pKa = 6.92GGHH10 pKa = 6.76DD11 pKa = 3.2QNVKK15 pKa = 10.59NEE17 pKa = 4.25EE18 pKa = 4.09NGHH21 pKa = 5.66DD22 pKa = 3.66VEE24 pKa = 5.74DD25 pKa = 4.39EE26 pKa = 4.12NGHH29 pKa = 6.38VEE31 pKa = 5.62DD32 pKa = 4.95GLCHH36 pKa = 6.5GGDD39 pKa = 3.35EE40 pKa = 4.58SGHH43 pKa = 6.13GEE45 pKa = 3.93EE46 pKa = 5.24NEE48 pKa = 4.0EE49 pKa = 4.52DD50 pKa = 3.53GHH52 pKa = 8.29GGEE55 pKa = 5.49DD56 pKa = 3.75EE57 pKa = 4.54NGHH60 pKa = 5.76DD61 pKa = 4.78VEE63 pKa = 5.85DD64 pKa = 4.37EE65 pKa = 3.99EE66 pKa = 5.17SAEE69 pKa = 4.09NGHH72 pKa = 6.23DD73 pKa = 3.82VEE75 pKa = 5.02DD76 pKa = 4.76AEE78 pKa = 4.73SGHH81 pKa = 7.07DD82 pKa = 3.77GEE84 pKa = 4.95SAEE87 pKa = 4.29NEE89 pKa = 4.38EE90 pKa = 4.44NGHH93 pKa = 6.84DD94 pKa = 3.86GEE96 pKa = 5.31DD97 pKa = 3.87EE98 pKa = 4.23NGHH101 pKa = 5.75DD102 pKa = 4.78VEE104 pKa = 5.85DD105 pKa = 4.37EE106 pKa = 3.99EE107 pKa = 5.17SAEE110 pKa = 4.09NGHH113 pKa = 6.23DD114 pKa = 3.82VEE116 pKa = 5.02DD117 pKa = 4.76AEE119 pKa = 4.73SGHH122 pKa = 7.07DD123 pKa = 3.77GEE125 pKa = 4.95SAEE128 pKa = 4.29NEE130 pKa = 4.38EE131 pKa = 4.46NGHH134 pKa = 6.34DD135 pKa = 4.01GEE137 pKa = 5.51NGLCHH142 pKa = 6.72GEE144 pKa = 4.09NGHH147 pKa = 6.68VEE149 pKa = 4.06DD150 pKa = 5.04GLWSEE155 pKa = 4.76ACHH158 pKa = 6.47SAGQMIEE165 pKa = 3.73ACLL168 pKa = 3.47

Molecular weight: 17.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H8ZGI3|H8ZGI3_NEMS1 Uncharacterized protein (Fragment) OS=Nematocida sp. 1 (strain ERTm2 / ATCC PRA-371) OX=944018 GN=NERG_02704 PE=4 SV=1
MM1 pKa = 7.52HH2 pKa = 7.54KK3 pKa = 10.51NNKK6 pKa = 8.7KK7 pKa = 10.02RR8 pKa = 11.84LFSARR13 pKa = 11.84LFSGTQKK20 pKa = 10.76GAVSLRR26 pKa = 11.84PVLYY30 pKa = 8.36RR31 pKa = 11.84TLPRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84LLLCSLALEE46 pKa = 4.44TACVYY51 pKa = 10.77VCVLHH56 pKa = 6.89RR57 pKa = 11.84RR58 pKa = 11.84NILCIEE64 pKa = 4.01RR65 pKa = 11.84RR66 pKa = 11.84SIVKK70 pKa = 8.6WCAWLKK76 pKa = 10.84SVAEE80 pKa = 3.96YY81 pKa = 10.77LILLQAIEE89 pKa = 4.58SGGSSEE95 pKa = 4.84RR96 pKa = 11.84AAKK99 pKa = 9.92KK100 pKa = 9.16KK101 pKa = 9.89KK102 pKa = 10.38KK103 pKa = 9.96YY104 pKa = 8.35MHH106 pKa = 6.72SLFLKK111 pKa = 9.15CVCRR115 pKa = 11.84CTDD118 pKa = 3.22RR119 pKa = 11.84VRR121 pKa = 11.84DD122 pKa = 3.54RR123 pKa = 11.84CAYY126 pKa = 7.06TRR128 pKa = 11.84CLCGHH133 pKa = 7.24RR134 pKa = 11.84LLAVHH139 pKa = 7.17ACLLHH144 pKa = 6.73LSGLALVLLFLSFEE158 pKa = 4.2EE159 pKa = 4.83HH160 pKa = 6.14VVCQLVSRR168 pKa = 11.84RR169 pKa = 11.84KK170 pKa = 8.98AAQLKK175 pKa = 10.15RR176 pKa = 11.84GLVLSWKK183 pKa = 10.13RR184 pKa = 11.84EE185 pKa = 3.55RR186 pKa = 11.84LVFGRR191 pKa = 11.84CLFFF195 pKa = 6.35

Molecular weight: 22.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2769

0

2769

997577

49

3290

360.3

41.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.719 ± 0.045

1.817 ± 0.02

4.761 ± 0.033

8.039 ± 0.055

4.041 ± 0.033

4.541 ± 0.04

2.1 ± 0.017

8.313 ± 0.046

8.285 ± 0.05

9.257 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.746 ± 0.021

4.971 ± 0.04

3.518 ± 0.031

3.258 ± 0.022

4.572 ± 0.031

7.985 ± 0.05

5.494 ± 0.035

5.864 ± 0.032

0.691 ± 0.01

4.027 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski