Klebsiella pneumoniae subsp. pneumoniae DSM 30104 = JCM 1662 = NBRC 14940

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Klebsiella; Klebsiella pneumoniae; Klebsiella pneumoniae subsp. pneumoniae

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4528 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J2DNB8|J2DNB8_KLEPN Transporter major facilitator family protein OS=Klebsiella pneumoniae subsp. pneumoniae DSM 30104 = JCM 1662 = NBRC 14940 OX=1162296 GN=UUU_12150 PE=4 SV=1
MM1 pKa = 7.46SGNIGANPVGVIKK14 pKa = 10.73DD15 pKa = 4.46GITATPTGFLGLSLEE30 pKa = 4.35YY31 pKa = 10.77AGGGEE36 pKa = 4.27FEE38 pKa = 5.29RR39 pKa = 11.84IGAGNYY45 pKa = 7.23MVVISSPTTEE55 pKa = 4.25GLIGSILNLNLGTSISMEE73 pKa = 4.05VTDD76 pKa = 3.36STKK79 pKa = 10.58YY80 pKa = 7.39VTGNVLHH87 pKa = 6.89TDD89 pKa = 2.98SDD91 pKa = 4.26GDD93 pKa = 3.74VGDD96 pKa = 4.09TGNEE100 pKa = 3.75ITVSSIQFGSQIEE113 pKa = 4.44TVTAGGVTINGTYY126 pKa = 8.21GTLIIKK132 pKa = 10.27SDD134 pKa = 3.27GSYY137 pKa = 10.47SYY139 pKa = 10.41HH140 pKa = 7.08ANGTTGVGQSDD151 pKa = 4.27VFTYY155 pKa = 10.04TITDD159 pKa = 3.88GLGHH163 pKa = 6.64TSSTTLTMNIEE174 pKa = 4.21STPPVAVDD182 pKa = 5.24DD183 pKa = 4.26IASLSAATAMQSVTWTQSVSNLAVPNTAVAANATSAAKK221 pKa = 8.88VTSQNFTIAEE231 pKa = 3.98GRR233 pKa = 11.84EE234 pKa = 3.73VDD236 pKa = 3.83GASITLNLSGNRR248 pKa = 11.84TSGLSLLNGDD258 pKa = 4.37LSGTVTLIHH267 pKa = 6.55HH268 pKa = 5.27VTVGGVTTDD277 pKa = 3.3VTVWTSSLTMALDD290 pKa = 4.26GSLLSPSSDD299 pKa = 2.91TDD301 pKa = 3.69TVTLNMVNGGIPLQLAAGDD320 pKa = 3.88YY321 pKa = 8.7TLSISHH327 pKa = 6.41TMHH330 pKa = 7.02AGTALSYY337 pKa = 11.03NLSASVAGTEE347 pKa = 4.1ILTDD351 pKa = 3.15AHH353 pKa = 5.99LTVGQSVAGNILDD366 pKa = 4.32GSDD369 pKa = 3.99ANGTPDD375 pKa = 3.43TLGSLHH381 pKa = 6.93SGFTISGEE389 pKa = 4.15NNLGIATNWTFHH401 pKa = 7.15SDD403 pKa = 3.17GTVTNDD409 pKa = 3.01TGTSASNGNAVLYY422 pKa = 10.62GEE424 pKa = 4.47YY425 pKa = 10.73GILTINGQGGYY436 pKa = 8.47TYY438 pKa = 10.82QLNGGVNTDD447 pKa = 4.27AITSKK452 pKa = 9.46EE453 pKa = 4.32TFTYY457 pKa = 10.44TLISSDD463 pKa = 3.81GGSSTANLTIDD474 pKa = 4.36LHH476 pKa = 5.23PQIAGSVNDD485 pKa = 4.66DD486 pKa = 3.97SVHH489 pKa = 4.89STAYY493 pKa = 10.62DD494 pKa = 3.36DD495 pKa = 3.91TFSMGVGADD504 pKa = 3.26TLVYY508 pKa = 10.96NLLADD513 pKa = 4.97DD514 pKa = 3.95NTGGNGSDD522 pKa = 2.74IWSDD526 pKa = 3.28FSVAQGDD533 pKa = 4.53HH534 pKa = 6.66IDD536 pKa = 3.59VSALLVGWNGSSDD549 pKa = 3.41TLGNYY554 pKa = 7.17ITLSYY559 pKa = 10.98VGGNTVVSIDD569 pKa = 3.69RR570 pKa = 11.84DD571 pKa = 3.8GTGGNTHH578 pKa = 6.04QPATLITLQGVHH590 pKa = 6.26INSLDD595 pKa = 3.72EE596 pKa = 6.3LIDD599 pKa = 3.88TNNSNN604 pKa = 3.77

Molecular weight: 61.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J2LLH4|J2LLH4_KLEPN Uncharacterized protein OS=Klebsiella pneumoniae subsp. pneumoniae DSM 30104 = JCM 1662 = NBRC 14940 OX=1162296 GN=UUU_36740 PE=4 SV=1
MM1 pKa = 7.6IRR3 pKa = 11.84LTLKK7 pKa = 10.43RR8 pKa = 11.84ATLVTSWSRR17 pKa = 11.84RR18 pKa = 11.84VKK20 pKa = 10.83LFLLPRR26 pKa = 11.84KK27 pKa = 7.36TLNSSKK33 pKa = 10.62HH34 pKa = 5.49VVLNWKK40 pKa = 9.38PNWLTFWQQLKK51 pKa = 10.49LVLSRR56 pKa = 11.84STPWNPP62 pKa = 3.15

Molecular weight: 7.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4528

0

4528

1291935

37

4234

285.3

31.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.138 ± 0.049

1.089 ± 0.014

5.036 ± 0.035

5.172 ± 0.038

4.007 ± 0.03

7.459 ± 0.04

2.527 ± 0.026

5.717 ± 0.038

3.972 ± 0.04

10.86 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.707 ± 0.02

3.642 ± 0.028

4.659 ± 0.03

4.744 ± 0.034

6.159 ± 0.041

5.798 ± 0.035

5.23 ± 0.032

6.905 ± 0.033

1.474 ± 0.02

2.705 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski