Methylobrevis pamukkalensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Pleomorphomonadaceae; Methylobrevis

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4503 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E3H8J4|A0A1E3H8J4_9RHIZ Leucine--tRNA ligase OS=Methylobrevis pamukkalensis OX=1439726 GN=leuS PE=3 SV=1
MM1 pKa = 7.77DD2 pKa = 5.28HH3 pKa = 7.16LFGGNEE9 pKa = 3.92GDD11 pKa = 3.91FLSGGSDD18 pKa = 3.76DD19 pKa = 3.7DD20 pKa = 3.97TLYY23 pKa = 11.57GEE25 pKa = 5.13AGNDD29 pKa = 3.61TLDD32 pKa = 3.75GGSGYY37 pKa = 8.73DD38 pKa = 3.69TLHH41 pKa = 6.65GGAGKK46 pKa = 10.38DD47 pKa = 3.44KK48 pKa = 10.77LYY50 pKa = 11.29GGADD54 pKa = 3.21SDD56 pKa = 3.86TYY58 pKa = 11.04YY59 pKa = 10.63INAAEE64 pKa = 4.05GDD66 pKa = 4.02EE67 pKa = 4.41VYY69 pKa = 11.12EE70 pKa = 4.12NANGGLDD77 pKa = 3.4HH78 pKa = 6.95VFVTGSLYY86 pKa = 10.35TLTANVEE93 pKa = 3.93RR94 pKa = 11.84GTLYY98 pKa = 10.3TAGGTLTGNAEE109 pKa = 4.08NNHH112 pKa = 6.05LFGSTGNEE120 pKa = 3.58TLYY123 pKa = 11.43GLGGHH128 pKa = 6.55DD129 pKa = 4.18TLDD132 pKa = 3.61GGIGADD138 pKa = 3.46TFYY141 pKa = 11.36GGAGDD146 pKa = 3.75DD147 pKa = 3.71TFIVEE152 pKa = 4.22NVGDD156 pKa = 4.11KK157 pKa = 10.74VIEE160 pKa = 4.03YY161 pKa = 10.5SGGGVDD167 pKa = 4.45TVITQLGNYY176 pKa = 7.4TLGDD180 pKa = 3.44HH181 pKa = 6.77VEE183 pKa = 3.96NLKK186 pKa = 10.7RR187 pKa = 11.84GNDD190 pKa = 3.82GAGKK194 pKa = 8.8LTGNGGANTITGNINGDD211 pKa = 4.08TIDD214 pKa = 4.22GGAGQDD220 pKa = 3.48VMIGGKK226 pKa = 10.64GNDD229 pKa = 3.66FYY231 pKa = 11.86YY232 pKa = 10.98VDD234 pKa = 4.79DD235 pKa = 5.52LGDD238 pKa = 3.91SVIEE242 pKa = 4.12KK243 pKa = 10.37AGEE246 pKa = 4.0GLDD249 pKa = 3.45EE250 pKa = 4.5VFASTAVYY258 pKa = 10.43TMTDD262 pKa = 2.94HH263 pKa = 7.55VEE265 pKa = 4.14QLHH268 pKa = 4.41VTYY271 pKa = 10.17TGGQISYY278 pKa = 11.43GNGIDD283 pKa = 3.4NWIDD287 pKa = 3.42GNTGNDD293 pKa = 3.14QMFGYY298 pKa = 10.26AGKK301 pKa = 10.28DD302 pKa = 3.18KK303 pKa = 11.12LVGEE307 pKa = 4.9GGDD310 pKa = 3.56DD311 pKa = 3.66LLYY314 pKa = 11.09GGSEE318 pKa = 4.33DD319 pKa = 4.43DD320 pKa = 4.81ALDD323 pKa = 3.9GGEE326 pKa = 5.56GEE328 pKa = 5.03DD329 pKa = 4.86LLDD332 pKa = 4.51GGTGADD338 pKa = 3.6TMLGGIGDD346 pKa = 4.26DD347 pKa = 3.45TYY349 pKa = 11.22MVDD352 pKa = 3.6DD353 pKa = 5.3AGDD356 pKa = 3.73KK357 pKa = 9.86VVEE360 pKa = 4.25MPDD363 pKa = 3.08GGFDD367 pKa = 3.5VVNASTSYY375 pKa = 10.38TLGNYY380 pKa = 9.36QIEE383 pKa = 4.33EE384 pKa = 4.2LVLTGANAIDD394 pKa = 3.79GTGNGGINHH403 pKa = 6.43IVGNNAANVLDD414 pKa = 4.22GQFGADD420 pKa = 3.27TLEE423 pKa = 4.54GKK425 pKa = 9.89DD426 pKa = 3.71GNDD429 pKa = 3.09TYY431 pKa = 11.83VVDD434 pKa = 3.88NVGDD438 pKa = 3.97KK439 pKa = 10.23TVEE442 pKa = 4.04TADD445 pKa = 3.41QGIDD449 pKa = 3.31TVLASASFSLKK460 pKa = 9.76GQYY463 pKa = 9.47VEE465 pKa = 5.18NLTLTGDD472 pKa = 3.55GDD474 pKa = 4.44INGTGNKK481 pKa = 9.58LDD483 pKa = 3.69NTIKK487 pKa = 10.99GNGAANVLDD496 pKa = 4.2GAEE499 pKa = 4.39GADD502 pKa = 3.74SLEE505 pKa = 4.52GGAGADD511 pKa = 2.87QFVFSTKK518 pKa = 10.34LGASNIDD525 pKa = 3.59TIVDD529 pKa = 4.34FKK531 pKa = 11.6AVDD534 pKa = 3.65DD535 pKa = 4.91TILLDD540 pKa = 3.82DD541 pKa = 6.01AIFKK545 pKa = 9.63TLDD548 pKa = 3.38LGALAAGEE556 pKa = 4.04FRR558 pKa = 11.84IGAAAKK564 pKa = 10.39DD565 pKa = 3.75ADD567 pKa = 4.01DD568 pKa = 3.77HH569 pKa = 7.0VIYY572 pKa = 10.68AKK574 pKa = 9.81GTGALYY580 pKa = 10.96YY581 pKa = 10.75DD582 pKa = 4.38ADD584 pKa = 4.16GNGAGAAIQFATLDD598 pKa = 3.6TGLSLTSADD607 pKa = 3.71FLVII611 pKa = 4.12

Molecular weight: 62.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E3H6A7|A0A1E3H6A7_9RHIZ Histidine kinase OS=Methylobrevis pamukkalensis OX=1439726 GN=envZ_2 PE=4 SV=1
MM1 pKa = 7.11TATVTAPLSIAVSSTPSVFRR21 pKa = 11.84QRR23 pKa = 11.84PVRR26 pKa = 11.84QVQSQSSTHH35 pKa = 6.06LFAVGQAVRR44 pKa = 11.84LKK46 pKa = 10.86GGFGHH51 pKa = 7.53PSQFAGASTSPPRR64 pKa = 11.84CRR66 pKa = 11.84RR67 pKa = 11.84AATRR71 pKa = 11.84PSIASATTTNATSASRR87 pKa = 11.84RR88 pKa = 11.84RR89 pKa = 11.84KK90 pKa = 9.76ASRR93 pKa = 11.84LPAPPLTVTARR104 pKa = 11.84II105 pKa = 3.84

Molecular weight: 11.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4503

0

4503

1213789

29

1730

269.6

28.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.644 ± 0.059

0.871 ± 0.013

6.099 ± 0.037

5.486 ± 0.03

3.53 ± 0.023

9.108 ± 0.044

1.985 ± 0.022

4.805 ± 0.033

2.519 ± 0.029

9.904 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.422 ± 0.019

2.061 ± 0.019

5.574 ± 0.033

2.409 ± 0.02

7.989 ± 0.053

5.184 ± 0.027

5.466 ± 0.03

7.787 ± 0.032

1.259 ± 0.016

1.899 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski