Drosophila obscura sigmavirus 10A

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sigmavirus; Drosophila obscura sigmavirus

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C8CJE8|C8CJE8_9RHAB Uncharacterized protein X OS=Drosophila obscura sigmavirus 10A OX=666962 GN=X PE=4 SV=1
MM1 pKa = 7.03STNPSKK7 pKa = 10.86PMNRR11 pKa = 11.84NSSEE15 pKa = 3.49KK16 pKa = 10.29DD17 pKa = 2.84IRR19 pKa = 11.84EE20 pKa = 4.0NSSSDD25 pKa = 3.26EE26 pKa = 4.08FEE28 pKa = 4.57EE29 pKa = 4.3EE30 pKa = 4.43VEE32 pKa = 4.23EE33 pKa = 4.38STSDD37 pKa = 3.49TEE39 pKa = 5.3KK40 pKa = 11.03NIPDD44 pKa = 5.43DD45 pKa = 3.75ISSPDD50 pKa = 3.57NEE52 pKa = 4.72FVPAPNQNIKK62 pKa = 10.59FSDD65 pKa = 3.42AFNSEE70 pKa = 4.13FGGKK74 pKa = 9.55YY75 pKa = 10.08NPEE78 pKa = 3.93EE79 pKa = 3.87LSKK82 pKa = 11.54YY83 pKa = 10.19GATDD87 pKa = 4.06DD88 pKa = 3.96YY89 pKa = 11.22CVAGRR94 pKa = 11.84LVQEE98 pKa = 4.24KK99 pKa = 10.93LNIKK103 pKa = 8.37EE104 pKa = 4.03TDD106 pKa = 3.18EE107 pKa = 5.25DD108 pKa = 3.84NVEE111 pKa = 4.0RR112 pKa = 11.84AILEE116 pKa = 4.13SSGVVPEE123 pKa = 5.08AEE125 pKa = 3.98AEE127 pKa = 4.06MEE129 pKa = 4.16EE130 pKa = 4.35LCEE133 pKa = 4.5KK134 pKa = 10.95SSDD137 pKa = 3.7EE138 pKa = 3.99NLEE141 pKa = 4.18CGATITLDD149 pKa = 4.12DD150 pKa = 5.08GFHH153 pKa = 6.36KK154 pKa = 10.31MKK156 pKa = 10.37IIASLEE162 pKa = 3.82EE163 pKa = 4.22RR164 pKa = 11.84FFDD167 pKa = 3.39PTQIHH172 pKa = 7.14LINRR176 pKa = 11.84FFRR179 pKa = 11.84EE180 pKa = 3.27LALYY184 pKa = 10.82VNTDD188 pKa = 3.26EE189 pKa = 4.63TEE191 pKa = 4.12GPILDD196 pKa = 3.53KK197 pKa = 11.12SKK199 pKa = 10.93NRR201 pKa = 11.84GAIVFGYY208 pKa = 10.37VEE210 pKa = 4.2SKK212 pKa = 9.8YY213 pKa = 10.54QKK215 pKa = 8.42KK216 pKa = 10.02QKK218 pKa = 7.61MTKK221 pKa = 10.18QNDD224 pKa = 3.5DD225 pKa = 4.68NIPSTSRR232 pKa = 11.84NCNKK236 pKa = 9.82NVCQPKK242 pKa = 9.89SPPPRR247 pKa = 11.84SEE249 pKa = 3.94GVAPAVNPNLQVILKK264 pKa = 9.76DD265 pKa = 3.73LNKK268 pKa = 9.97GLKK271 pKa = 8.03WKK273 pKa = 10.51HH274 pKa = 6.25FITQKK279 pKa = 10.35EE280 pKa = 4.3LSFKK284 pKa = 10.05MGQTGMTVEE293 pKa = 4.78EE294 pKa = 4.13ISAVLEE300 pKa = 4.23AGNEE304 pKa = 3.91IKK306 pKa = 10.52SGKK309 pKa = 10.17EE310 pKa = 3.33YY311 pKa = 11.15VEE313 pKa = 3.92YY314 pKa = 10.54LFKK317 pKa = 10.72IRR319 pKa = 11.84GHH321 pKa = 6.14FNQLEE326 pKa = 3.89ASYY329 pKa = 10.24IYY331 pKa = 10.51QPP333 pKa = 3.7

Molecular weight: 37.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C8CJF0|C8CJF0_9RHAB Glycoprotein OS=Drosophila obscura sigmavirus 10A OX=666962 GN=G PE=4 SV=1
MM1 pKa = 7.37SKK3 pKa = 10.57KK4 pKa = 9.96EE5 pKa = 4.61AIPSLALMSTCIEE18 pKa = 4.09GKK20 pKa = 10.09EE21 pKa = 4.12EE22 pKa = 4.12IASSPHH28 pKa = 6.08SSFFVPTAPVNDD40 pKa = 4.47SIPYY44 pKa = 8.08YY45 pKa = 9.88VHH47 pKa = 6.69NLTVEE52 pKa = 4.13AEE54 pKa = 3.87ISVNSNRR61 pKa = 11.84VIDD64 pKa = 3.43TWEE67 pKa = 4.0PLRR70 pKa = 11.84EE71 pKa = 4.02ILTSWVDD78 pKa = 3.44NYY80 pKa = 11.02KK81 pKa = 11.0GPLNVKK87 pKa = 9.99SILGVVYY94 pKa = 9.62TLLIFKK100 pKa = 8.2MKK102 pKa = 10.11KK103 pKa = 8.3MRR105 pKa = 11.84VGVNYY110 pKa = 10.5LMYY113 pKa = 10.17GIKK116 pKa = 9.56MFDD119 pKa = 3.57RR120 pKa = 11.84ISFLMTYY127 pKa = 9.82NFEE130 pKa = 4.03NSIPVQGEE138 pKa = 3.94LNYY141 pKa = 10.7KK142 pKa = 9.66SGRR145 pKa = 11.84GQFLYY150 pKa = 10.13TVNFKK155 pKa = 10.93YY156 pKa = 10.47RR157 pKa = 11.84LTPIKK162 pKa = 10.34RR163 pKa = 11.84HH164 pKa = 5.37GSLIQTLFSPGVVDD178 pKa = 3.32MSKK181 pKa = 10.4QVGPTLAVALDD192 pKa = 3.93SIGIKK197 pKa = 9.36TKK199 pKa = 10.34EE200 pKa = 4.09DD201 pKa = 3.49PAGLVLLYY209 pKa = 7.51TTT211 pKa = 4.93

Molecular weight: 23.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4052

211

2146

675.3

77.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.763 ± 0.769

1.234 ± 0.159

5.01 ± 0.198

6.318 ± 0.881

4.22 ± 0.175

5.503 ± 0.353

2.468 ± 0.356

7.28 ± 0.481

6.885 ± 0.338

10.242 ± 0.692

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.295 ± 0.324

5.528 ± 0.378

4.541 ± 0.34

3.258 ± 0.297

4.837 ± 0.518

8.119 ± 0.365

6.145 ± 0.29

5.602 ± 0.483

1.9 ± 0.255

3.85 ± 0.318
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski