Shuangao sobemo-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEF6|A0A1L3KEF6_9VIRU RdRP_1 domain-containing protein OS=Shuangao sobemo-like virus 2 OX=1923475 PE=4 SV=1
MM1 pKa = 7.88LSMMRR6 pKa = 11.84NSVQVVPMAASALAGAAAYY25 pKa = 9.52QVGKK29 pKa = 10.06IASQAYY35 pKa = 9.83DD36 pKa = 3.5SLKK39 pKa = 10.82SPPAPPTFYY48 pKa = 10.9EE49 pKa = 3.96QLLEE53 pKa = 4.1MGLEE57 pKa = 4.12RR58 pKa = 11.84KK59 pKa = 8.28KK60 pKa = 9.46TIAVALFGAAAVGFWACKK78 pKa = 7.56NRR80 pKa = 11.84RR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84VLLKK87 pKa = 10.27PLSQEE92 pKa = 3.99SLVAGSAEE100 pKa = 4.12MPALEE105 pKa = 4.94PACQVAIAFEE115 pKa = 4.7SPDD118 pKa = 3.27GTLVKK123 pKa = 10.43VGSGVRR129 pKa = 11.84GLVGCNHH136 pKa = 5.75VLFTAAHH143 pKa = 5.88NLSFEE148 pKa = 4.11QPLWLIKK155 pKa = 10.38GSKK158 pKa = 7.26QVRR161 pKa = 11.84LGDD164 pKa = 3.65YY165 pKa = 10.95SPIPLAADD173 pKa = 3.47AALLLVPEE181 pKa = 4.62KK182 pKa = 10.48TFGLLGVKK190 pKa = 9.07VANFSPLPGKK200 pKa = 10.17SVAVSVTGLAGKK212 pKa = 8.29GTTGILSPVVNPHH225 pKa = 6.3HH226 pKa = 7.06GIGHH230 pKa = 4.74VTYY233 pKa = 10.52AATTAGGYY241 pKa = 9.83SGAPYY246 pKa = 10.5VSGSQVYY253 pKa = 9.93GIHH256 pKa = 5.91IHH258 pKa = 6.32GGMRR262 pKa = 11.84NGGFEE267 pKa = 3.92ILYY270 pKa = 10.13LYY272 pKa = 10.97ALAKK276 pKa = 10.04IALEE280 pKa = 3.98YY281 pKa = 10.6TEE283 pKa = 5.69EE284 pKa = 4.06DD285 pKa = 3.56TEE287 pKa = 4.41DD288 pKa = 3.55AFMEE292 pKa = 4.29RR293 pKa = 11.84LFNSDD298 pKa = 3.51DD299 pKa = 3.43PDD301 pKa = 4.19YY302 pKa = 10.96YY303 pKa = 10.58VQEE306 pKa = 4.41VGDD309 pKa = 3.83KK310 pKa = 10.9VIVRR314 pKa = 11.84VHH316 pKa = 6.21GNSHH320 pKa = 4.95YY321 pKa = 10.9HH322 pKa = 5.7VVDD325 pKa = 3.59AKK327 pKa = 10.86KK328 pKa = 10.53FRR330 pKa = 11.84DD331 pKa = 3.83YY332 pKa = 10.75EE333 pKa = 4.29ARR335 pKa = 11.84WEE337 pKa = 4.09NADD340 pKa = 3.78YY341 pKa = 11.39SSDD344 pKa = 3.97DD345 pKa = 3.68YY346 pKa = 12.09VEE348 pKa = 4.64EE349 pKa = 4.45CASEE353 pKa = 4.03ALNFQSPGQRR363 pKa = 11.84SRR365 pKa = 11.84AGPSIEE371 pKa = 4.16CLHH374 pKa = 6.33RR375 pKa = 11.84QPQLVTSSCQTSALPEE391 pKa = 4.14SSPSSDD397 pKa = 4.38RR398 pKa = 11.84RR399 pKa = 11.84PQDD402 pKa = 3.19PSNKK406 pKa = 9.32RR407 pKa = 11.84LKK409 pKa = 10.7KK410 pKa = 10.45LINSRR415 pKa = 11.84LRR417 pKa = 11.84RR418 pKa = 11.84LQLKK422 pKa = 9.28TNGTRR427 pKa = 11.84STPSRR432 pKa = 11.84SLVTQVV438 pKa = 2.91

Molecular weight: 47.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEF6|A0A1L3KEF6_9VIRU RdRP_1 domain-containing protein OS=Shuangao sobemo-like virus 2 OX=1923475 PE=4 SV=1
MM1 pKa = 7.63CFGSFKK7 pKa = 10.61LPKK10 pKa = 9.68PGTAVPGWTLYY21 pKa = 10.53RR22 pKa = 11.84VLAPTATAGNKK33 pKa = 8.73LVPNEE38 pKa = 3.38RR39 pKa = 11.84VARR42 pKa = 11.84VFAEE46 pKa = 4.32LRR48 pKa = 11.84PTAPGPIKK56 pKa = 10.3QAFEE60 pKa = 4.02EE61 pKa = 4.39ADD63 pKa = 3.73KK64 pKa = 10.39LTAPPTTAKK73 pKa = 10.55DD74 pKa = 3.39QWDD77 pKa = 3.61SFYY80 pKa = 8.83TQSVARR86 pKa = 11.84HH87 pKa = 5.81ASLKK91 pKa = 10.13TYY93 pKa = 10.48VEE95 pKa = 3.77PDD97 pKa = 2.95EE98 pKa = 5.73RR99 pKa = 11.84LFSMILYY106 pKa = 9.61QAEE109 pKa = 4.15QTYY112 pKa = 10.51SSCIRR117 pKa = 11.84KK118 pKa = 9.04LPLGWDD124 pKa = 3.5TRR126 pKa = 11.84SHH128 pKa = 6.63FDD130 pKa = 2.86KK131 pKa = 10.75TLRR134 pKa = 11.84KK135 pKa = 9.41LDD137 pKa = 3.74RR138 pKa = 11.84TSSPGWPLCKK148 pKa = 9.58QASTIGDD155 pKa = 3.58WLYY158 pKa = 10.45PGSILDD164 pKa = 4.32PDD166 pKa = 3.92PMKK169 pKa = 10.98AEE171 pKa = 4.21QLWMMVQQVFEE182 pKa = 4.5LEE184 pKa = 4.06YY185 pKa = 9.88EE186 pKa = 4.15HH187 pKa = 6.8FFKK190 pKa = 11.22VFIKK194 pKa = 10.36PEE196 pKa = 3.69PHH198 pKa = 6.78KK199 pKa = 10.6EE200 pKa = 4.03EE201 pKa = 4.23KK202 pKa = 10.48SLARR206 pKa = 11.84RR207 pKa = 11.84WRR209 pKa = 11.84LIMASSLPVQIAWHH223 pKa = 5.7MAMGHH228 pKa = 5.94LEE230 pKa = 3.94EE231 pKa = 5.35AFLRR235 pKa = 11.84EE236 pKa = 4.16QPFIPLAYY244 pKa = 10.03GEE246 pKa = 4.56PFFAGSWLRR255 pKa = 11.84FKK257 pKa = 10.67EE258 pKa = 4.29SCKK261 pKa = 9.27KK262 pKa = 9.22TKK264 pKa = 9.02ITWATDD270 pKa = 2.87KK271 pKa = 11.19KK272 pKa = 10.39AWDD275 pKa = 3.69WNSPGWVYY283 pKa = 8.89QACRR287 pKa = 11.84EE288 pKa = 4.06LRR290 pKa = 11.84VRR292 pKa = 11.84LTRR295 pKa = 11.84FDD297 pKa = 3.64HH298 pKa = 6.39THH300 pKa = 6.26DD301 pKa = 3.25QQRR304 pKa = 11.84WFRR307 pKa = 11.84VVNWLYY313 pKa = 11.36DD314 pKa = 3.45DD315 pKa = 5.28AYY317 pKa = 10.84RR318 pKa = 11.84SSKK321 pKa = 10.61LLLASGHH328 pKa = 6.08VYY330 pKa = 10.08QQTTAGLMKK339 pKa = 10.37SGLVPTISDD348 pKa = 3.31NSLSQDD354 pKa = 4.47LIDD357 pKa = 4.04MAAQLSVGRR366 pKa = 11.84LPCKK370 pKa = 10.34KK371 pKa = 9.88RR372 pKa = 11.84VTGDD376 pKa = 3.48DD377 pKa = 3.97VIQEE381 pKa = 4.28KK382 pKa = 9.64PADD385 pKa = 3.78PQAYY389 pKa = 7.66IQRR392 pKa = 11.84VQSFGCKK399 pKa = 8.8IKK401 pKa = 10.55QAATKK406 pKa = 10.46LEE408 pKa = 3.97FMGFDD413 pKa = 3.38MDD415 pKa = 5.28GDD417 pKa = 4.14LTPIYY422 pKa = 8.8PQKK425 pKa = 10.67HH426 pKa = 5.21LWNFLHH432 pKa = 6.58QKK434 pKa = 10.02EE435 pKa = 4.69EE436 pKa = 4.06YY437 pKa = 10.13LEE439 pKa = 4.19QVVGAYY445 pKa = 10.23LRR447 pKa = 11.84IYY449 pKa = 10.56ANSPQHH455 pKa = 4.98SQFWRR460 pKa = 11.84AVADD464 pKa = 3.79KK465 pKa = 11.25LNLRR469 pKa = 11.84VMSPSFYY476 pKa = 10.9SFFMNNPEE484 pKa = 3.94AAMKK488 pKa = 10.1LGPVRR493 pKa = 11.84YY494 pKa = 9.36RR495 pKa = 11.84EE496 pKa = 4.08GLLGGAGLVV505 pKa = 3.59

Molecular weight: 58.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

943

438

505

471.5

52.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.332 ± 0.765

1.379 ± 0.006

4.56 ± 0.444

5.302 ± 0.033

4.136 ± 0.764

6.681 ± 1.006

2.439 ± 0.047

3.287 ± 0.06

5.832 ± 0.679

9.756 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.439 ± 0.401

2.545 ± 0.426

6.469 ± 0.199

4.878 ± 0.502

5.726 ± 0.161

7.741 ± 1.059

4.772 ± 0.284

6.787 ± 1.086

2.121 ± 0.939

3.818 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski