Bacillus sp. FJAT-26390

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6340 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B8VXQ4|A0A1B8VXQ4_9BACI Uncharacterized protein OS=Bacillus sp. FJAT-26390 OX=1743142 GN=A7975_23905 PE=4 SV=1
MM1 pKa = 8.06PITKK5 pKa = 9.59PFMAARR11 pKa = 11.84RR12 pKa = 11.84FSATAADD19 pKa = 4.09GTGVGAAYY27 pKa = 10.53NILATATTNDD37 pKa = 3.39AGVAPTAFPTAPAYY51 pKa = 10.38YY52 pKa = 9.93NLYY55 pKa = 10.29INAQIQTADD64 pKa = 3.5TSSVTTTAITIPDD77 pKa = 4.51GDD79 pKa = 4.36TLDD82 pKa = 4.09PATPIVIEE90 pKa = 4.2FVVNN94 pKa = 3.98

Molecular weight: 9.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B8W851|A0A1B8W851_9BACI Uncharacterized protein OS=Bacillus sp. FJAT-26390 OX=1743142 GN=A7975_17580 PE=4 SV=1
MM1 pKa = 7.54LARR4 pKa = 11.84VGGFDD9 pKa = 5.38GIMSMMGKK17 pKa = 8.81VQQFFGIFQQIRR29 pKa = 11.84PAFKK33 pKa = 9.83MVGSLFGTKK42 pKa = 10.17AFLSGVPSSRR52 pKa = 11.84VRR54 pKa = 11.84SKK56 pKa = 11.22NKK58 pKa = 9.35KK59 pKa = 9.35AKK61 pKa = 10.05LNSRR65 pKa = 11.84AAAASRR71 pKa = 11.84QKK73 pKa = 9.78TSRR76 pKa = 11.84KK77 pKa = 9.07RR78 pKa = 11.84SSRR81 pKa = 3.4

Molecular weight: 8.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6340

0

6340

2129051

26

6751

335.8

37.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.631 ± 0.037

0.675 ± 0.008

5.148 ± 0.021

6.594 ± 0.036

4.232 ± 0.023

7.278 ± 0.034

2.058 ± 0.016

6.914 ± 0.029

5.554 ± 0.031

10.051 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.783 ± 0.017

3.995 ± 0.025

3.903 ± 0.02

3.866 ± 0.02

4.663 ± 0.028

6.534 ± 0.024

5.409 ± 0.031

6.918 ± 0.024

1.32 ± 0.014

3.472 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski