Neptunomonas qingdaonensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Neptunomonas

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4011 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1I2LMB4|A0A1I2LMB4_9GAMM Simple sugar transport system substrate-binding protein OS=Neptunomonas qingdaonensis OX=1045558 GN=SAMN05216175_10138 PE=3 SV=1
NN1 pKa = 7.77GIQDD5 pKa = 3.53TGEE8 pKa = 4.26GGVAGAKK15 pKa = 9.95VSLFNCDD22 pKa = 2.22GTAANDD28 pKa = 3.48INGNPVASITTGGDD42 pKa = 3.27GIYY45 pKa = 10.72NFTNLAPGEE54 pKa = 4.35YY55 pKa = 8.92YY56 pKa = 10.98VQFTAPSGYY65 pKa = 10.88VITQQLATEE74 pKa = 4.6ADD76 pKa = 3.55AAGDD80 pKa = 4.0DD81 pKa = 4.1SDD83 pKa = 5.71ANAAGITSCVVLEE96 pKa = 4.26SGEE99 pKa = 4.17TEE101 pKa = 3.98EE102 pKa = 5.73RR103 pKa = 11.84VDD105 pKa = 5.0AGLVLQNAGIDD116 pKa = 3.19IEE118 pKa = 4.63KK119 pKa = 10.01YY120 pKa = 9.35VRR122 pKa = 11.84GRR124 pKa = 11.84YY125 pKa = 8.81EE126 pKa = 4.17GEE128 pKa = 4.02SGTDD132 pKa = 3.05EE133 pKa = 4.49GLTPGFWKK141 pKa = 8.45THH143 pKa = 5.13SEE145 pKa = 4.16FGPAPLAGWPEE156 pKa = 4.35SGFDD160 pKa = 3.48PTDD163 pKa = 3.34SYY165 pKa = 12.1EE166 pKa = 4.78SIFSVEE172 pKa = 4.28VPGTPSLLDD181 pKa = 3.66ALNSGGGDD189 pKa = 3.29LDD191 pKa = 3.43ALMRR195 pKa = 11.84HH196 pKa = 5.28STAALLNASNPFVAYY211 pKa = 9.2EE212 pKa = 3.73YY213 pKa = 10.77SQAEE217 pKa = 4.88VISMTQAAISSGVYY231 pKa = 10.21EE232 pKa = 3.89ITKK235 pKa = 10.62DD236 pKa = 3.18LFAAKK241 pKa = 10.26NEE243 pKa = 4.18LGADD247 pKa = 3.79LNEE250 pKa = 4.5GTSEE254 pKa = 4.03GTIVEE259 pKa = 4.73TIDD262 pKa = 3.57YY263 pKa = 10.68DD264 pKa = 4.26ADD266 pKa = 4.13TPDD269 pKa = 3.88GPYY272 pKa = 10.13IPIDD276 pKa = 3.45GEE278 pKa = 4.5AIFTYY283 pKa = 10.63VVTNTGDD290 pKa = 3.22VALSDD295 pKa = 3.78VQVTDD300 pKa = 4.13DD301 pKa = 3.47RR302 pKa = 11.84LANVTLVGGDD312 pKa = 3.68TNNDD316 pKa = 2.95NLLDD320 pKa = 3.92VGEE323 pKa = 4.38TWTYY327 pKa = 7.95TASEE331 pKa = 4.37TVVAGGTFVNTGTVTGADD349 pKa = 3.01ATGRR353 pKa = 11.84ILSDD357 pKa = 3.4SDD359 pKa = 3.55LAHH362 pKa = 6.67YY363 pKa = 7.51NTSFLTSSLGDD374 pKa = 3.78RR375 pKa = 11.84VWLDD379 pKa = 3.56LDD381 pKa = 3.91SDD383 pKa = 4.93GIQDD387 pKa = 3.48NDD389 pKa = 3.32EE390 pKa = 4.53AGVAGVTVNLLDD402 pKa = 3.69SDD404 pKa = 4.66GNVSATTITDD414 pKa = 3.26DD415 pKa = 3.37YY416 pKa = 11.58GNYY419 pKa = 10.16LFVALEE425 pKa = 4.14PGEE428 pKa = 4.12YY429 pKa = 9.69AIQVVAPDD437 pKa = 3.68GTVFTSQNQGMDD449 pKa = 3.79DD450 pKa = 4.51DD451 pKa = 5.18RR452 pKa = 11.84DD453 pKa = 3.67SDD455 pKa = 4.0VASNGVSEE463 pKa = 4.6FTTLTAGEE471 pKa = 4.86NDD473 pKa = 4.01LSWDD477 pKa = 3.44AGLVPTGTIDD487 pKa = 2.85IEE489 pKa = 4.27KK490 pKa = 9.71FVRR493 pKa = 11.84IEE495 pKa = 3.97TPGDD499 pKa = 3.51FFEE502 pKa = 5.88GAVCDD507 pKa = 4.05EE508 pKa = 4.57FGKK511 pKa = 9.37ATALTFDD518 pKa = 4.27YY519 pKa = 11.12EE520 pKa = 4.13EE521 pKa = 4.31STNVSTSQDD530 pKa = 3.05SSKK533 pKa = 11.53AKK535 pKa = 10.17ILTGTTFDD543 pKa = 4.51GDD545 pKa = 3.54GKK547 pKa = 10.99SYY549 pKa = 11.27VIVTDD554 pKa = 3.93KK555 pKa = 11.31SDD557 pKa = 3.42PTDD560 pKa = 3.64LGGKK564 pKa = 9.39VYY566 pKa = 10.45FSGYY570 pKa = 8.07VVEE573 pKa = 5.39GEE575 pKa = 4.43EE576 pKa = 4.34FTATAADD583 pKa = 3.55AGADD587 pKa = 3.31RR588 pKa = 11.84FGSNTFVHH596 pKa = 7.02FFDD599 pKa = 6.39DD600 pKa = 3.97NPFDD604 pKa = 4.16NVVGDD609 pKa = 4.69DD610 pKa = 5.7DD611 pKa = 5.46LLQSLTYY618 pKa = 8.87HH619 pKa = 6.46TSCSQPIYY627 pKa = 10.76LGDD630 pKa = 3.46QVGNVTLVGYY640 pKa = 10.02SGEE643 pKa = 4.35SGTAPSSPPVIGPDD657 pKa = 3.48LDD659 pKa = 4.96ADD661 pKa = 4.75TIAEE665 pKa = 4.41GPIGTIGTDD674 pKa = 3.29TAVFTYY680 pKa = 10.07KK681 pKa = 10.5VKK683 pKa = 9.67NTGTSDD689 pKa = 3.66LEE691 pKa = 4.37NVLVTDD697 pKa = 4.61DD698 pKa = 4.41RR699 pKa = 11.84LSEE702 pKa = 3.89LTLINKK708 pKa = 9.62GDD710 pKa = 3.91GDD712 pKa = 3.86NLLEE716 pKa = 4.9VGEE719 pKa = 4.32TWIYY723 pKa = 8.19TASEE727 pKa = 4.01AVLGGINTNIGKK739 pKa = 8.31VTANVVGTQFGVNDD753 pKa = 3.55QDD755 pKa = 3.31AANYY759 pKa = 10.3VGFDD763 pKa = 3.7TPPSIDD769 pKa = 3.19LEE771 pKa = 4.56KK772 pKa = 9.58YY773 pKa = 10.21VKK775 pKa = 10.79VEE777 pKa = 4.11GYY779 pKa = 8.57TPFVPMVCEE788 pKa = 4.27TYY790 pKa = 10.66GKK792 pKa = 8.53PVEE795 pKa = 4.06MTFVYY800 pKa = 10.33EE801 pKa = 3.99VDD803 pKa = 4.56DD804 pKa = 4.87DD805 pKa = 4.55NEE807 pKa = 4.98SISNTQEE814 pKa = 3.4GKK816 pKa = 10.79AKK818 pKa = 10.26ILTGTTLDD826 pKa = 3.86NDD828 pKa = 3.46DD829 pKa = 4.45LAWVRR834 pKa = 11.84VTDD837 pKa = 3.53SDD839 pKa = 4.03KK840 pKa = 11.62AFDD843 pKa = 4.19GGGKK847 pKa = 9.27IYY849 pKa = 10.8FEE851 pKa = 4.18GLVAKK856 pKa = 10.35GADD859 pKa = 3.83FTATAALAGADD870 pKa = 3.42RR871 pKa = 11.84FASSTYY877 pKa = 9.54IHH879 pKa = 6.56YY880 pKa = 10.61FDD882 pKa = 6.11DD883 pKa = 4.16EE884 pKa = 5.66ADD886 pKa = 3.62FTTEE890 pKa = 3.55NSGGLLQSLTYY901 pKa = 8.9HH902 pKa = 6.35TSCSKK907 pKa = 10.57PINLGDD913 pKa = 3.38EE914 pKa = 3.88TGYY917 pKa = 11.38AKK919 pKa = 10.73LVGYY923 pKa = 10.21VGEE926 pKa = 5.26DD927 pKa = 3.45GAAEE931 pKa = 3.89PVNEE935 pKa = 4.21YY936 pKa = 11.28GEE938 pKa = 4.79DD939 pKa = 3.69ADD941 pKa = 4.59EE942 pKa = 5.2GPGPEE947 pKa = 3.95AAFGSTVEE955 pKa = 4.05FTYY958 pKa = 11.06VVTNTGSTALGQIEE972 pKa = 4.85LSDD975 pKa = 3.97DD976 pKa = 4.29RR977 pKa = 11.84ISGLTLVGGDD987 pKa = 4.22DD988 pKa = 5.53DD989 pKa = 5.81GDD991 pKa = 3.75NLLDD995 pKa = 4.91VGEE998 pKa = 4.33EE999 pKa = 3.98WLFTGSEE1006 pKa = 4.33TAVSGQVTNIGKK1018 pKa = 8.44VTGTAVDD1025 pKa = 3.55EE1026 pKa = 4.93KK1027 pKa = 11.16GNSLNLPTVTDD1038 pKa = 4.05SDD1040 pKa = 3.72PGNYY1044 pKa = 9.07VVVGGPSIDD1053 pKa = 3.29VEE1055 pKa = 4.49KK1056 pKa = 10.55YY1057 pKa = 9.61VKK1059 pKa = 10.74VEE1061 pKa = 4.04GGGVPYY1067 pKa = 10.28EE1068 pKa = 4.15ALVCDD1073 pKa = 4.5TYY1075 pKa = 11.86GKK1077 pKa = 10.33ALAMTFRR1084 pKa = 11.84YY1085 pKa = 10.28LEE1087 pKa = 4.43GSTVDD1092 pKa = 3.29TDD1094 pKa = 3.42QDD1096 pKa = 3.57SGKK1099 pKa = 10.88AYY1101 pKa = 9.52IDD1103 pKa = 3.91KK1104 pKa = 11.01DD1105 pKa = 4.12LGTDD1109 pKa = 3.61DD1110 pKa = 6.02DD1111 pKa = 4.56GVSYY1115 pKa = 10.69IIVSDD1120 pKa = 3.6KK1121 pKa = 11.21SSPTDD1126 pKa = 3.41LSGKK1130 pKa = 10.06VYY1132 pKa = 10.34FQGEE1136 pKa = 4.39VAKK1139 pKa = 10.78GADD1142 pKa = 3.94FTALGASSFSSSTYY1156 pKa = 9.35IHH1158 pKa = 6.58YY1159 pKa = 10.64FDD1161 pKa = 6.1DD1162 pKa = 4.33SPFDD1166 pKa = 3.82GEE1168 pKa = 4.48GSIDD1172 pKa = 3.87LLQSSAYY1179 pKa = 8.26HH1180 pKa = 6.0TSCSQPINIGDD1191 pKa = 3.77VVGNTTLVGYY1201 pKa = 10.27LGEE1204 pKa = 5.09DD1205 pKa = 3.91GEE1207 pKa = 4.79ANPTGSNDD1215 pKa = 3.88GLGDD1219 pKa = 4.48DD1220 pKa = 5.35ADD1222 pKa = 4.25TGPGPDD1228 pKa = 3.44AAFGSDD1234 pKa = 3.27VVFNYY1239 pKa = 10.03VVTNTGSGALKK1250 pKa = 10.68NVVLTDD1256 pKa = 3.69DD1257 pKa = 5.06RR1258 pKa = 11.84ISDD1261 pKa = 3.6VTYY1264 pKa = 11.07VSGDD1268 pKa = 3.71DD1269 pKa = 3.66GDD1271 pKa = 4.27KK1272 pKa = 10.82LLEE1275 pKa = 4.43VGEE1278 pKa = 4.26EE1279 pKa = 3.8WLYY1282 pKa = 10.27TATEE1286 pKa = 4.06KK1287 pKa = 10.86AGQGVIGNIGKK1298 pKa = 8.04VTAKK1302 pKa = 10.45DD1303 pKa = 3.25AATGTISVMDD1313 pKa = 4.32KK1314 pKa = 10.98DD1315 pKa = 3.46AAFYY1319 pKa = 10.75NGSAPGITLGDD1330 pKa = 3.26RR1331 pKa = 11.84VWFDD1335 pKa = 3.83KK1336 pKa = 11.42NCDD1339 pKa = 3.67GIQDD1343 pKa = 4.03DD1344 pKa = 5.14GEE1346 pKa = 4.85NGAAGITVNLMNSVGNVIATTTTDD1370 pKa = 2.49VDD1372 pKa = 3.71GNYY1375 pKa = 10.36LFTDD1379 pKa = 4.73LAPGTYY1385 pKa = 9.85SVGFVAPNGYY1395 pKa = 9.66IFTKK1399 pKa = 10.44QDD1401 pKa = 2.76ATADD1405 pKa = 4.01DD1406 pKa = 4.11VDD1408 pKa = 4.49SDD1410 pKa = 4.26ANANGLTGQYY1420 pKa = 10.37KK1421 pKa = 10.33LAGGEE1426 pKa = 4.23TNLTVDD1432 pKa = 3.28AGLVEE1437 pKa = 4.8FTTKK1441 pKa = 10.54DD1442 pKa = 3.17VSFSFNGSSSGSGTYY1457 pKa = 11.04GNIRR1461 pKa = 11.84TYY1463 pKa = 10.36TDD1465 pKa = 2.7NGVSVNVSAHH1475 pKa = 5.22SRR1477 pKa = 11.84SDD1479 pKa = 3.56KK1480 pKa = 11.22SGDD1483 pKa = 2.86WSTSYY1488 pKa = 11.07LGAFSGGMGVTNRR1501 pKa = 11.84NEE1503 pKa = 4.17SGSGNSHH1510 pKa = 5.37TVDD1513 pKa = 3.24NRR1515 pKa = 11.84GDD1517 pKa = 3.29MDD1519 pKa = 5.16DD1520 pKa = 3.79YY1521 pKa = 11.92VLFEE1525 pKa = 4.13FDD1527 pKa = 3.47QNVVIDD1533 pKa = 4.13SAFLGYY1539 pKa = 10.6VVDD1542 pKa = 5.0DD1543 pKa = 3.91SDD1545 pKa = 3.6MSVWIGTLDD1554 pKa = 3.41NAFNNHH1560 pKa = 5.4TNLSDD1565 pKa = 5.94DD1566 pKa = 4.41ILSQMGFTEE1575 pKa = 4.34INN1577 pKa = 3.09

Molecular weight:
165.19 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1I2LQ04|A0A1I2LQ04_9GAMM NADP-dependent 3-hydroxy acid dehydrogenase YdfG OS=Neptunomonas qingdaonensis OX=1045558 GN=SAMN05216175_101181 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.31SGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.27GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight:
5.05 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4011

0

4011

1312960

24

7596

327.3

36.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.019 ± 0.049

1.027 ± 0.015

5.611 ± 0.052

6.08 ± 0.045

3.931 ± 0.025

7.001 ± 0.047

2.264 ± 0.022

6.43 ± 0.03

4.824 ± 0.042

10.735 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.596 ± 0.028

3.922 ± 0.039

4.081 ± 0.027

4.477 ± 0.031

4.964 ± 0.042

6.714 ± 0.042

5.321 ± 0.063

6.928 ± 0.033

1.241 ± 0.016

2.833 ± 0.023

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski