Salmonella phage vB_SPuM_SP116

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ounavirinae; Felixounavirus; Salmonella virus SP116

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 126 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D4D9P8|A0A0D4D9P8_9CAUD Uncharacterized protein OS=Salmonella phage vB_SPuM_SP116 OX=1567025 GN=HB2014_29 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 9.8CFHH5 pKa = 6.61GTTQEE10 pKa = 3.86NFTNLINNGDD20 pKa = 4.02KK21 pKa = 10.86PSGAWNCSDD30 pKa = 3.4MDD32 pKa = 4.16GNFYY36 pKa = 10.85VYY38 pKa = 10.1PEE40 pKa = 3.92NKK42 pKa = 9.59IYY44 pKa = 10.85GDD46 pKa = 3.74DD47 pKa = 3.62AEE49 pKa = 4.9EE50 pKa = 4.7IISEE54 pKa = 4.98GIQQALGNAAITAAFQMKK72 pKa = 7.87TQNIVILEE80 pKa = 4.02LDD82 pKa = 3.41IPEE85 pKa = 5.73DD86 pKa = 4.11EE87 pKa = 5.46LNDD90 pKa = 5.32DD91 pKa = 4.11YY92 pKa = 12.13SCDD95 pKa = 3.52NMSGVASFTEE105 pKa = 4.18YY106 pKa = 11.07FDD108 pKa = 3.6ISWIKK113 pKa = 10.27KK114 pKa = 9.43VYY116 pKa = 6.93TTEE119 pKa = 3.93FNAMFSPFCLPSLDD133 pKa = 4.3NPNLNYY139 pKa = 9.49IDD141 pKa = 4.36EE142 pKa = 4.48SLEE145 pKa = 3.85LLAKK149 pKa = 10.14SVQQSDD155 pKa = 4.51SIQVFCDD162 pKa = 2.88IMDD165 pKa = 4.27TLTEE169 pKa = 4.29NIIEE173 pKa = 4.4RR174 pKa = 11.84DD175 pKa = 3.28LKK177 pKa = 11.43GFFF180 pKa = 4.04

Molecular weight: 20.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D4DAS3|A0A0D4DAS3_9CAUD Uncharacterized protein OS=Salmonella phage vB_SPuM_SP116 OX=1567025 GN=HB2014_23 PE=4 SV=1
MM1 pKa = 7.73AIIKK5 pKa = 10.2NVVITAKK12 pKa = 9.81TRR14 pKa = 11.84DD15 pKa = 3.97DD16 pKa = 3.75ARR18 pKa = 11.84VMAKK22 pKa = 9.91KK23 pKa = 10.63LCGKK27 pKa = 10.42VIDD30 pKa = 4.54NGKK33 pKa = 9.57QSAVRR38 pKa = 11.84WGVKK42 pKa = 9.54ADD44 pKa = 3.59KK45 pKa = 10.27QLKK48 pKa = 9.59LKK50 pKa = 10.58NSPINLFTCVNTIGKK65 pKa = 7.8TNVYY69 pKa = 8.44TKK71 pKa = 9.84KK72 pKa = 10.55AYY74 pKa = 9.18IRR76 pKa = 11.84RR77 pKa = 11.84VALTSPIRR85 pKa = 11.84TMRR88 pKa = 11.84SYY90 pKa = 11.89AKK92 pKa = 10.62LKK94 pKa = 10.69INN96 pKa = 3.95

Molecular weight: 10.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

126

0

126

25246

34

910

200.4

22.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.94 ± 0.272

1.307 ± 0.107

6.29 ± 0.192

6.948 ± 0.266

4.215 ± 0.156

6.472 ± 0.192

1.984 ± 0.14

6.072 ± 0.145

8.057 ± 0.266

7.799 ± 0.152

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.896 ± 0.117

5.367 ± 0.193

2.927 ± 0.133

3.383 ± 0.156

4.167 ± 0.174

6.413 ± 0.229

6.148 ± 0.257

7.063 ± 0.17

1.208 ± 0.101

4.345 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski