Leminorella grimontii ATCC 33999 = DSM 5078

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Budviciaceae; Leminorella; Leminorella grimontii

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3756 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A085HGK5|A0A085HGK5_9GAMM Pyruvate dehydrogenase complex transcriptional repressor OS=Leminorella grimontii ATCC 33999 = DSM 5078 OX=1005999 GN=GLGR_2168 PE=4 SV=1
VV1 pKa = 6.23ATDD4 pKa = 3.34AAGNEE9 pKa = 4.63SPASTTFEE17 pKa = 4.2LTVDD21 pKa = 4.25TVAPAAPIITSIVDD35 pKa = 3.46NLSPTTGPIVAGVATNDD52 pKa = 4.01PAPTLNGRR60 pKa = 11.84AEE62 pKa = 4.12AGAIVTIFDD71 pKa = 4.77GEE73 pKa = 4.46DD74 pKa = 3.26NPIGSTTADD83 pKa = 4.47DD84 pKa = 3.8NGDD87 pKa = 2.92WHH89 pKa = 6.3FTPDD93 pKa = 3.41EE94 pKa = 4.15PLGEE98 pKa = 4.49GDD100 pKa = 4.39HH101 pKa = 6.67EE102 pKa = 4.35LTAVATDD109 pKa = 3.25AAGNVGGASPFVAITIDD126 pKa = 3.41TTAPDD131 pKa = 4.01APTGLSVSPSGTRR144 pKa = 11.84VTGSAEE150 pKa = 3.92PGSTVTITDD159 pKa = 3.14ADD161 pKa = 4.05GTVLGSATADD171 pKa = 3.39GTGSFTATITPAQTNGEE188 pKa = 4.25SLLAFAQDD196 pKa = 3.1KK197 pKa = 10.66AGNVGEE203 pKa = 4.24AAGFSASTTGLPDD216 pKa = 3.04VPTIEE221 pKa = 4.32TVTDD225 pKa = 3.48NVSPITGPLEE235 pKa = 4.3NGQSTNEE242 pKa = 4.06TLPLLSGKK250 pKa = 9.43AQAGATVTVYY260 pKa = 11.11DD261 pKa = 4.09KK262 pKa = 11.11GAEE265 pKa = 4.05IGTTTADD272 pKa = 5.48DD273 pKa = 4.33DD274 pKa = 4.72GVWNFTPTVPLTEE287 pKa = 4.42GQHH290 pKa = 5.54VFTATSTNASGTGGFSEE307 pKa = 4.35PRR309 pKa = 11.84SIVVDD314 pKa = 4.88TIAPDD319 pKa = 3.43APTATISADD328 pKa = 2.77GSMISGIAEE337 pKa = 4.21AGSTVTITLANGTTVTTAANDD358 pKa = 3.69SGSYY362 pKa = 10.45SYY364 pKa = 10.44TFQSKK369 pKa = 7.41QTDD372 pKa = 4.01GEE374 pKa = 4.45QLFVTATDD382 pKa = 3.55AAGNPSPRR390 pKa = 11.84TQADD394 pKa = 3.67APDD397 pKa = 4.48LPLSANNNVIEE408 pKa = 4.69LALTSDD414 pKa = 3.96APVTTTQYY422 pKa = 11.35GDD424 pKa = 3.35YY425 pKa = 11.2GFLLVGALGNLASVLGNDD443 pKa = 3.67TAQVTFTVGNGSTADD458 pKa = 3.55VKK460 pKa = 10.73IDD462 pKa = 3.47ADD464 pKa = 3.4ATGIVLSLLSSLEE477 pKa = 4.12LVVQLHH483 pKa = 6.11NSANDD488 pKa = 2.8SWTTVVDD495 pKa = 3.94TQAPRR500 pKa = 11.84WASLLTFGDD509 pKa = 3.64SGISLNLDD517 pKa = 3.01ALAAGTYY524 pKa = 9.79RR525 pKa = 11.84VVSYY529 pKa = 8.38NTSLLATGSYY539 pKa = 10.1TSLNVSVIEE548 pKa = 4.29TGAGTVNGDD557 pKa = 3.54TAHH560 pKa = 6.53SGNVILDD567 pKa = 4.18DD568 pKa = 4.94DD569 pKa = 4.65PVSGSDD575 pKa = 3.36TASGGALVTQVTNAQGTVVPVTADD599 pKa = 3.23GAVIQGTYY607 pKa = 8.79GTLTLYY613 pKa = 10.4PDD615 pKa = 3.86GSYY618 pKa = 10.21TYY620 pKa = 10.7TLFNTSASVIGHH632 pKa = 6.82KK633 pKa = 10.64DD634 pKa = 3.09SFSYY638 pKa = 9.99TLSDD642 pKa = 3.36GTISDD647 pKa = 3.8TASLVISLGEE657 pKa = 3.98GAVTGQAVAVDD668 pKa = 4.16NVASLLFDD676 pKa = 3.84TEE678 pKa = 4.06VATIDD683 pKa = 3.66NGTWSQKK690 pKa = 10.45GYY692 pKa = 8.28TVVNLGLGDD701 pKa = 4.01ALDD704 pKa = 3.86VGVLDD709 pKa = 5.27DD710 pKa = 4.79LSNPIVFNVEE720 pKa = 3.51QGTTRR725 pKa = 11.84SLTLQSEE732 pKa = 4.72VGGIALASKK741 pKa = 10.48FDD743 pKa = 3.76LYY745 pKa = 10.49IYY747 pKa = 10.26KK748 pKa = 10.49FNDD751 pKa = 2.94EE752 pKa = 4.23TQKK755 pKa = 9.85YY756 pKa = 8.31DD757 pKa = 3.09QYY759 pKa = 11.81KK760 pKa = 9.79RR761 pKa = 11.84VEE763 pKa = 4.38DD764 pKa = 3.57WLEE767 pKa = 3.86APLFGGKK774 pKa = 8.91SDD776 pKa = 4.46EE777 pKa = 5.47LDD779 pKa = 3.31LTLDD783 pKa = 3.41GGKK786 pKa = 10.1YY787 pKa = 10.43LFLLDD792 pKa = 3.85TTSGLSVLTGYY803 pKa = 8.74SLKK806 pKa = 10.74VLEE809 pKa = 4.23DD810 pKa = 3.26HH811 pKa = 6.8VYY813 pKa = 10.86SVTSVSASTSGEE825 pKa = 3.99VLDD828 pKa = 5.73NDD830 pKa = 3.57IAPPNTVVSAVNGVSVAASGDD851 pKa = 3.85TVIVGQYY858 pKa = 7.75GTLTINAQGHH868 pKa = 4.24YY869 pKa = 9.48TYY871 pKa = 10.07TLKK874 pKa = 10.97SGVGADD880 pKa = 4.22SISTPDD886 pKa = 3.24SFIYY890 pKa = 10.15TITAPNGDD898 pKa = 4.35RR899 pKa = 11.84DD900 pKa = 4.03TASLNVTPAPRR911 pKa = 11.84GLDD914 pKa = 3.26AVDD917 pKa = 4.25DD918 pKa = 4.27VSAAMTVTSIQDD930 pKa = 3.15TSGYY934 pKa = 10.24SDD936 pKa = 3.37TSVGTASWSSSLGKK950 pKa = 7.52TTGNGSGTFVVDD962 pKa = 3.66EE963 pKa = 4.54GSALKK968 pKa = 10.69DD969 pKa = 2.97IVLTFKK975 pKa = 11.0ASSTLLLGSADD986 pKa = 3.46ISWEE990 pKa = 3.81ILEE993 pKa = 4.51GTTSIRR999 pKa = 11.84SGTTSASMLLGGSPGTVTLSGLEE1022 pKa = 4.1LDD1024 pKa = 4.12AGTYY1028 pKa = 6.98TLKK1031 pKa = 10.1FTGSEE1036 pKa = 4.01TGLVGQITVTPSVSGTLVDD1055 pKa = 4.4LSDD1058 pKa = 3.86YY1059 pKa = 11.15LSTAGHH1065 pKa = 6.03AVSGNIFDD1073 pKa = 4.82GSDD1076 pKa = 2.95SAGAMDD1082 pKa = 3.92QLSSVGTTLSITDD1095 pKa = 3.78ANGVTTTLDD1104 pKa = 3.47PYY1106 pKa = 8.41TTSNATASIHH1116 pKa = 5.2GQYY1119 pKa = 8.93GTLTIGVDD1127 pKa = 3.36GAYY1130 pKa = 10.34SYY1132 pKa = 9.93TLNPAVSLSTITEE1145 pKa = 4.16KK1146 pKa = 10.39EE1147 pKa = 4.27TFNYY1151 pKa = 9.77TLTSSNGQTANATLTVDD1168 pKa = 3.81MAPQFVSSGHH1178 pKa = 6.1NDD1180 pKa = 3.53VITGTAYY1187 pKa = 10.49ADD1189 pKa = 3.31TAIYY1193 pKa = 10.24HH1194 pKa = 6.82LLNAASNTGGNGSDD1208 pKa = 2.9VWSNFSLQQGDD1219 pKa = 4.14KK1220 pKa = 10.52IDD1222 pKa = 3.38IHH1224 pKa = 6.3EE1225 pKa = 5.38LLVGWNGQTSTLGNYY1240 pKa = 10.48LSVTTSGNDD1249 pKa = 3.29TVISIDD1255 pKa = 3.4RR1256 pKa = 11.84DD1257 pKa = 3.58GASGQTYY1264 pKa = 10.27QSTTLITLEE1273 pKa = 4.19NVHH1276 pKa = 5.7TTLDD1280 pKa = 3.55EE1281 pKa = 5.28LIEE1284 pKa = 3.9QHH1286 pKa = 7.08HH1287 pKa = 7.22IITHH1291 pKa = 5.9

Molecular weight: 131.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A085HBX3|A0A085HBX3_9GAMM DNA gyrase subunit B OS=Leminorella grimontii ATCC 33999 = DSM 5078 OX=1005999 GN=gyrB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.62RR12 pKa = 11.84NRR14 pKa = 11.84AHH16 pKa = 7.59GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84SRR41 pKa = 11.84LTVAGG46 pKa = 3.91

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3756

0

3756

1211632

37

3852

322.6

35.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.623 ± 0.047

1.079 ± 0.016

5.344 ± 0.033

5.778 ± 0.042

3.799 ± 0.032

7.947 ± 0.067

1.971 ± 0.018

5.838 ± 0.037

4.478 ± 0.036

10.458 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.643 ± 0.023

3.871 ± 0.043

4.22 ± 0.035

4.013 ± 0.032

5.512 ± 0.042

6.535 ± 0.041

5.346 ± 0.042

7.176 ± 0.029

1.335 ± 0.017

3.035 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski