Alternanthera yellow vein virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4GZI6|Q4GZI6_9GEMI Transcriptional activator protein OS=Alternanthera yellow vein virus OX=337826 GN=ac2 PE=3 SV=1
MM1 pKa = 7.42RR2 pKa = 11.84QPGRR6 pKa = 11.84FRR8 pKa = 11.84LNRR11 pKa = 11.84KK12 pKa = 8.78NFFLTYY18 pKa = 8.51SQCPISKK25 pKa = 10.43EE26 pKa = 3.82EE27 pKa = 4.38ALEE30 pKa = 3.8QLLNIQTPVNKK41 pKa = 10.14KK42 pKa = 9.77FIRR45 pKa = 11.84VCRR48 pKa = 11.84EE49 pKa = 3.34LHH51 pKa = 6.55EE52 pKa = 5.57DD53 pKa = 3.97GNPHH57 pKa = 6.43LHH59 pKa = 6.93ALVQFEE65 pKa = 4.92GKK67 pKa = 10.14FDD69 pKa = 3.56LTNPRR74 pKa = 11.84FFDD77 pKa = 3.64LQSPNSSSQYY87 pKa = 10.25HH88 pKa = 6.03CFITDD93 pKa = 3.38AKK95 pKa = 10.73SSSDD99 pKa = 3.1VKK101 pKa = 11.12AYY103 pKa = 9.75IEE105 pKa = 4.26KK106 pKa = 10.91DD107 pKa = 3.08GDD109 pKa = 3.6ILDD112 pKa = 3.87WGTFQIDD119 pKa = 3.03GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 3.71AYY134 pKa = 10.81ARR136 pKa = 11.84AINTGNKK143 pKa = 9.39DD144 pKa = 3.15QALDD148 pKa = 3.97VIKK151 pKa = 10.51EE152 pKa = 4.1LAPKK156 pKa = 10.3DD157 pKa = 3.74YY158 pKa = 11.2VLQFHH163 pKa = 6.76NLNANLEE170 pKa = 4.56RR171 pKa = 11.84IFMPPIEE178 pKa = 4.63EE179 pKa = 4.31YY180 pKa = 10.23ISPFNCSSFDD190 pKa = 3.55QVPEE194 pKa = 4.52EE195 pKa = 4.12IEE197 pKa = 3.61EE198 pKa = 4.14WAADD202 pKa = 3.64NVMSAAARR210 pKa = 11.84PWRR213 pKa = 11.84PISIVIEE220 pKa = 4.23GDD222 pKa = 3.2SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.7NYY239 pKa = 10.01LCGHH243 pKa = 7.13IDD245 pKa = 3.96LSPRR249 pKa = 11.84VYY251 pKa = 11.1SNEE254 pKa = 3.04AWYY257 pKa = 10.37NIIDD261 pKa = 4.53DD262 pKa = 4.1VDD264 pKa = 3.43PHH266 pKa = 5.9YY267 pKa = 11.29VKK269 pKa = 10.59HH270 pKa = 6.1FKK272 pKa = 10.84EE273 pKa = 4.16FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.6YY287 pKa = 10.57GKK289 pKa = 9.12PVQIKK294 pKa = 10.41GGIPTIFLCNPGPTSSYY311 pKa = 11.31KK312 pKa = 10.67EE313 pKa = 3.89FLDD316 pKa = 3.57EE317 pKa = 4.57EE318 pKa = 4.61KK319 pKa = 11.2NIGLKK324 pKa = 9.82NWAIKK329 pKa = 10.38NATFVTINQPLYY341 pKa = 10.4SGSHH345 pKa = 4.95QSPTQASQEE354 pKa = 4.2EE355 pKa = 4.63TSQTEE360 pKa = 4.1GG361 pKa = 3.22

Molecular weight: 41.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4GZI9|Q4GZI9_9GEMI Protein V2 OS=Alternanthera yellow vein virus OX=337826 GN=av2 PE=3 SV=1
MM1 pKa = 7.6SKK3 pKa = 10.24RR4 pKa = 11.84AADD7 pKa = 3.97MIISSSGSRR16 pKa = 11.84VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.06SPMARR29 pKa = 11.84RR30 pKa = 11.84ATAPIVRR37 pKa = 11.84ATRR40 pKa = 11.84KK41 pKa = 7.64QQWSSRR47 pKa = 11.84PMYY50 pKa = 10.05RR51 pKa = 11.84KK52 pKa = 8.59PRR54 pKa = 11.84LYY56 pKa = 10.8RR57 pKa = 11.84MYY59 pKa = 10.37RR60 pKa = 11.84SPDD63 pKa = 3.16VPYY66 pKa = 10.77GCEE69 pKa = 4.42GPCKK73 pKa = 10.15VQSFEE78 pKa = 4.21KK79 pKa = 10.35KK80 pKa = 9.61HH81 pKa = 6.23DD82 pKa = 3.73VSHH85 pKa = 6.9TGTLLCVSDD94 pKa = 3.64VTRR97 pKa = 11.84GSGITHH103 pKa = 6.35RR104 pKa = 11.84VGKK107 pKa = 9.68RR108 pKa = 11.84FCIKK112 pKa = 10.14SIYY115 pKa = 9.87IIGKK119 pKa = 8.24IWMDD123 pKa = 3.52EE124 pKa = 4.09NIKK127 pKa = 10.71SKK129 pKa = 10.61NHH131 pKa = 5.85TNTVMFWLVRR141 pKa = 11.84DD142 pKa = 3.99RR143 pKa = 11.84RR144 pKa = 11.84PVTTPYY150 pKa = 11.3GFGEE154 pKa = 4.51AFNMYY159 pKa = 10.55DD160 pKa = 4.21NEE162 pKa = 4.43PSTATIKK169 pKa = 10.93NDD171 pKa = 3.08LRR173 pKa = 11.84DD174 pKa = 3.68RR175 pKa = 11.84LQVLHH180 pKa = 6.91RR181 pKa = 11.84FSATVTGGQYY191 pKa = 10.85ASKK194 pKa = 8.76EE195 pKa = 3.54QAMIKK200 pKa = 10.19RR201 pKa = 11.84FWRR204 pKa = 11.84INNHH208 pKa = 4.03VVYY211 pKa = 10.65NHH213 pKa = 5.67QEE215 pKa = 3.37KK216 pKa = 10.24AAYY219 pKa = 8.25EE220 pKa = 4.07NHH222 pKa = 6.29TEE224 pKa = 4.02NALVLYY230 pKa = 7.23MACTHH235 pKa = 7.04ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.66FYY251 pKa = 11.18DD252 pKa = 3.06SVMNN256 pKa = 4.52

Molecular weight: 29.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1096

96

361

182.7

20.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.657 ± 0.79

2.372 ± 0.484

4.927 ± 0.613

4.471 ± 0.825

4.015 ± 0.664

4.745 ± 0.597

3.65 ± 0.513

6.387 ± 0.836

4.927 ± 0.667

7.117 ± 1.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.555 ± 0.71

5.566 ± 0.872

5.748 ± 0.827

5.292 ± 0.688

7.208 ± 1.118

7.573 ± 1.136

6.843 ± 1.191

5.292 ± 0.988

1.46 ± 0.268

4.197 ± 0.487
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski