Erwinia phage vB_EamM_Joad

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Risingsunvirus; unclassified Risingsunvirus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 245 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A223LH95|A0A223LH95_9CAUD Uncharacterized protein OS=Erwinia phage vB_EamM_Joad OX=2026081 GN=JOAD_138 PE=4 SV=1
MM1 pKa = 7.4TLNLVKK7 pKa = 9.63THH9 pKa = 6.43EE10 pKa = 4.54EE11 pKa = 3.79NTTPAFTIITVDD23 pKa = 3.12RR24 pKa = 11.84FGEE27 pKa = 4.12EE28 pKa = 3.8TLCYY32 pKa = 10.11VDD34 pKa = 3.71QDD36 pKa = 3.99EE37 pKa = 4.85FLGEE41 pKa = 3.92ALLSFDD47 pKa = 4.37EE48 pKa = 5.16DD49 pKa = 3.67GSTRR53 pKa = 11.84FGVLYY58 pKa = 10.46DD59 pKa = 3.6NNEE62 pKa = 3.93THH64 pKa = 5.99SQIMMVNYY72 pKa = 9.9FSGDD76 pKa = 3.33LEE78 pKa = 4.17MLKK81 pKa = 10.66LVAYY85 pKa = 10.13HH86 pKa = 6.73NDD88 pKa = 3.29SEE90 pKa = 5.54LVLTCCIEE98 pKa = 4.26NEE100 pKa = 4.14DD101 pKa = 4.55GADD104 pKa = 3.29KK105 pKa = 10.89RR106 pKa = 11.84LNDD109 pKa = 3.52VVDD112 pKa = 4.52FLNSSEE118 pKa = 4.28KK119 pKa = 10.87LLMINGDD126 pKa = 3.28VWTEE130 pKa = 3.53ILSAYY135 pKa = 8.52GNCMDD140 pKa = 5.66RR141 pKa = 11.84FQQEE145 pKa = 3.45AA146 pKa = 3.36

Molecular weight: 16.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223LHK3|A0A223LHK3_9CAUD Uncharacterized protein OS=Erwinia phage vB_EamM_Joad OX=2026081 GN=JOAD_211 PE=4 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84KK3 pKa = 8.5WFSVLGRR10 pKa = 11.84YY11 pKa = 8.32IAAQYY16 pKa = 10.79QLGLKK21 pKa = 10.13RR22 pKa = 11.84ILLNHH27 pKa = 6.23HH28 pKa = 6.0MRR30 pKa = 11.84RR31 pKa = 11.84LIKK34 pKa = 10.54KK35 pKa = 8.31EE36 pKa = 3.53DD37 pKa = 3.89RR38 pKa = 11.84IIQEE42 pKa = 3.88FEE44 pKa = 3.83RR45 pKa = 11.84ALTAQGKK52 pKa = 8.81ALRR55 pKa = 11.84NRR57 pKa = 11.84GYY59 pKa = 11.13LSRR62 pKa = 11.84QSLKK66 pKa = 10.85HH67 pKa = 5.34LTLHH71 pKa = 6.99LADD74 pKa = 4.36EE75 pKa = 4.55LHH77 pKa = 5.77MQKK80 pKa = 10.17CQIRR84 pKa = 11.84KK85 pKa = 7.26VTVEE89 pKa = 4.78LIDD92 pKa = 3.71GDD94 pKa = 4.04FFIDD98 pKa = 5.44LEE100 pKa = 4.4IATHH104 pKa = 5.59NRR106 pKa = 11.84TVKK109 pKa = 10.56RR110 pKa = 11.84MVIVV114 pKa = 3.7

Molecular weight: 13.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

245

0

245

74489

33

2561

304.0

34.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.943 ± 0.215

0.91 ± 0.048

6.294 ± 0.092

6.205 ± 0.127

4.299 ± 0.077

6.393 ± 0.169

1.967 ± 0.078

6.0 ± 0.101

6.173 ± 0.15

8.913 ± 0.132

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.957 ± 0.089

5.798 ± 0.122

4.01 ± 0.092

3.754 ± 0.081

4.802 ± 0.096

5.989 ± 0.12

6.506 ± 0.116

6.978 ± 0.148

1.118 ± 0.045

3.993 ± 0.098
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski