bacterium HR35

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 609 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H6A8W0|A0A2H6A8W0_9BACT Methionine aminopeptidase OS=bacterium HR35 OX=2035430 GN=map PE=3 SV=1
MM1 pKa = 7.5KK2 pKa = 10.19KK3 pKa = 9.58NHH5 pKa = 7.0PKK7 pKa = 10.61LSTRR11 pKa = 11.84FYY13 pKa = 10.88LLSTRR18 pKa = 11.84FLVLLLLISMFFFGYY33 pKa = 9.17PVIWKK38 pKa = 9.54NPRR41 pKa = 11.84IPPKK45 pKa = 10.29IKK47 pKa = 9.69EE48 pKa = 4.01AQAAISFIGSVEE60 pKa = 3.82GSAANGGDD68 pKa = 3.5VTLSLSGLGLQEE80 pKa = 4.2GDD82 pKa = 3.95LVIVAYY88 pKa = 10.34AIGDD92 pKa = 3.57NDD94 pKa = 3.22NVNFNMAILSPAGYY108 pKa = 8.62TEE110 pKa = 4.19VADD113 pKa = 3.97LHH115 pKa = 8.01SDD117 pKa = 3.44DD118 pKa = 4.6TQDD121 pKa = 3.33VDD123 pKa = 5.24LGVYY127 pKa = 8.15WKK129 pKa = 11.11VMGATPDD136 pKa = 3.29TSVTVDD142 pKa = 3.33GLGGTDD148 pKa = 3.14ASVAAVAMAFRR159 pKa = 11.84GVDD162 pKa = 3.21PATPMDD168 pKa = 4.89VIPTTATGINTMHH181 pKa = 7.44PNPPAIDD188 pKa = 3.78YY189 pKa = 10.59VSTGVWTVIAGASGHH204 pKa = 6.54ALNLANTNNPYY215 pKa = 9.38TFPSGYY221 pKa = 7.31TTDD224 pKa = 3.25NVQRR228 pKa = 11.84PEE230 pKa = 4.06NDD232 pKa = 3.24TSDD235 pKa = 3.53VTVGLGYY242 pKa = 8.84RR243 pKa = 11.84TNPADD248 pKa = 3.91PEE250 pKa = 4.54DD251 pKa = 4.53PGVMTHH257 pKa = 6.58SGTDD261 pKa = 3.29STSYY265 pKa = 10.22SWAAVTIALRR275 pKa = 11.84PKK277 pKa = 10.13PPSTFNQSAYY287 pKa = 10.85RR288 pKa = 11.84FFNNLDD294 pKa = 3.62STDD297 pKa = 3.55VGSPLANQNTPASLTSAGQAFRR319 pKa = 11.84LRR321 pKa = 11.84LLIHH325 pKa = 6.52VGTSNLLEE333 pKa = 4.32NEE335 pKa = 4.15QSFKK339 pKa = 11.28LQFGTSTGSSCTSSPPASWADD360 pKa = 3.49VTTSTPIAFNDD371 pKa = 3.55NPTPADD377 pKa = 3.72GATLTANTNDD387 pKa = 3.77PTHH390 pKa = 6.37GADD393 pKa = 3.23IIVNQTYY400 pKa = 10.56EE401 pKa = 4.04EE402 pKa = 4.65LNNFTNSVSSINAGEE417 pKa = 4.09DD418 pKa = 3.76GKK420 pKa = 10.3WDD422 pKa = 4.48FSLKK426 pKa = 10.98DD427 pKa = 3.28NGAPAGTTYY436 pKa = 10.91CLRR439 pKa = 11.84VVKK442 pKa = 10.72ADD444 pKa = 3.34GTLLDD449 pKa = 4.22TYY451 pKa = 10.98SVIPQITTAAAPILSAYY468 pKa = 10.44RR469 pKa = 11.84FFNNLDD475 pKa = 3.62STDD478 pKa = 3.55VGSPLANQDD487 pKa = 2.98TAATLTSAGQAFRR500 pKa = 11.84LRR502 pKa = 11.84LLLHH506 pKa = 6.64LPSGLNFNSPTSSNNLIEE524 pKa = 4.89SGLIPAGSGGGRR536 pKa = 11.84IRR538 pKa = 11.84IAAPTPNRR546 pKa = 11.84LYY548 pKa = 10.81LVYY551 pKa = 11.0YY552 pKa = 11.02DD553 pKa = 4.02MTNLDD558 pKa = 3.86LRR560 pKa = 11.84FCRR563 pKa = 11.84STNGGVSWSCNAIEE577 pKa = 4.32STGDD581 pKa = 3.24VGVTPDD587 pKa = 4.66IYY589 pKa = 11.42ALDD592 pKa = 3.97EE593 pKa = 4.26NNIFITHH600 pKa = 6.94RR601 pKa = 11.84DD602 pKa = 3.32ATNGNLRR609 pKa = 11.84FCKK612 pKa = 10.29SSNGGLNFSCSILEE626 pKa = 4.39AGWQGFDD633 pKa = 3.68SSIKK637 pKa = 10.44AISTSTIFVSYY648 pKa = 10.81APAPANFSYY657 pKa = 10.77SILRR661 pKa = 11.84FCRR664 pKa = 11.84TTDD667 pKa = 4.37GGNTWNCSDD676 pKa = 3.96VEE678 pKa = 4.61GNTTTQYY685 pKa = 10.9GWYY688 pKa = 9.52SSLDD692 pKa = 3.41AVDD695 pKa = 4.11SNNIYY700 pKa = 9.49IAHH703 pKa = 6.64YY704 pKa = 10.68DD705 pKa = 4.06GINLDD710 pKa = 3.88LRR712 pKa = 11.84FCRR715 pKa = 11.84TSNGGTTWNCGVIEE729 pKa = 4.26TTGDD733 pKa = 2.97IGRR736 pKa = 11.84KK737 pKa = 8.77PSIKK741 pKa = 10.43AFNNNYY747 pKa = 9.82IYY749 pKa = 10.24IAHH752 pKa = 7.31RR753 pKa = 11.84DD754 pKa = 3.47EE755 pKa = 4.75TNNQLRR761 pKa = 11.84FCRR764 pKa = 11.84TTDD767 pKa = 4.24GGTSWNCNAIEE778 pKa = 3.98IGGFSSSLKK787 pKa = 10.42IVDD790 pKa = 3.53KK791 pKa = 10.78DD792 pKa = 3.66VVYY795 pKa = 10.39IAHH798 pKa = 7.18FDD800 pKa = 3.53FTNASIRR807 pKa = 11.84LCNTNNGGQTWSCSTIANAPPTLTSGEE834 pKa = 4.19NLSLSLDD841 pKa = 3.66LLDD844 pKa = 4.96PYY846 pKa = 9.99MIYY849 pKa = 10.38VAYY852 pKa = 10.29GFTTDD857 pKa = 2.84SSNEE861 pKa = 3.56VRR863 pKa = 11.84LYY865 pKa = 10.75KK866 pKa = 10.04YY867 pKa = 9.07TGNDD871 pKa = 3.25FFKK874 pKa = 10.76LQYY877 pKa = 10.83SVMSGTCDD885 pKa = 2.86TSFSGEE891 pKa = 4.09VYY893 pKa = 10.82NDD895 pKa = 3.11VGFDD899 pKa = 3.58TPIAFYY905 pKa = 11.43NNNTTSDD912 pKa = 3.35ASTPTTNTNDD922 pKa = 3.65PTHH925 pKa = 5.84GTDD928 pKa = 3.28TIVYY932 pKa = 7.19QTYY935 pKa = 9.81KK936 pKa = 10.09EE937 pKa = 4.28KK938 pKa = 11.04KK939 pKa = 9.12FFTNRR944 pKa = 11.84TNIFSGSDD952 pKa = 3.61GLWDD956 pKa = 4.63FVLRR960 pKa = 11.84DD961 pKa = 3.68NNAPSNTTYY970 pKa = 11.0CLRR973 pKa = 11.84VVKK976 pKa = 10.7ADD978 pKa = 3.32GSLLQGYY985 pKa = 7.97NVIPQITTAAAAPITCTFSATSTSFSSLSPSAVSTSSPDD1024 pKa = 2.9ITITVTSSAGFIISVNDD1041 pKa = 3.73AGNGTNPGLYY1051 pKa = 9.97KK1052 pKa = 9.93STSPTYY1058 pKa = 10.58LIPSPNSSYY1067 pKa = 10.65TATATLVAGVDD1078 pKa = 4.06GYY1080 pKa = 11.39GIQATTTNSNISINPRR1096 pKa = 11.84YY1097 pKa = 9.19NVSGDD1102 pKa = 3.6TVGGLTTTTITLASSSVAVSSAQITIKK1129 pKa = 10.61HH1130 pKa = 5.51KK1131 pKa = 10.75AAVSSLAPTGNYY1143 pKa = 9.55QDD1145 pKa = 4.51TITYY1149 pKa = 10.21SCTSPP1154 pKa = 3.49

Molecular weight: 122.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H6A9N1|A0A2H6A9N1_9BACT Ribosome-recycling factor OS=bacterium HR35 OX=2035430 GN=frr PE=3 SV=1
MM1 pKa = 7.68PSKK4 pKa = 10.83AQIEE8 pKa = 4.37RR9 pKa = 11.84WKK11 pKa = 10.74KK12 pKa = 8.32EE13 pKa = 3.94PKK15 pKa = 9.95FKK17 pKa = 9.65TRR19 pKa = 11.84KK20 pKa = 9.33RR21 pKa = 11.84NFCFRR26 pKa = 11.84CGRR29 pKa = 11.84TRR31 pKa = 11.84GYY33 pKa = 10.73FRR35 pKa = 11.84DD36 pKa = 3.85FNLCRR41 pKa = 11.84ICLRR45 pKa = 11.84EE46 pKa = 3.65MAHH49 pKa = 6.67RR50 pKa = 11.84GEE52 pKa = 4.19IPGLKK57 pKa = 9.06KK58 pKa = 10.87ASWW61 pKa = 3.28

Molecular weight: 7.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

609

0

609

183829

36

2003

301.9

34.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.555 ± 0.085

0.687 ± 0.031

4.198 ± 0.062

8.174 ± 0.147

6.507 ± 0.127

5.476 ± 0.089

1.19 ± 0.04

9.57 ± 0.1

10.57 ± 0.18

11.53 ± 0.145

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.233 ± 0.03

5.792 ± 0.097

3.803 ± 0.065

2.853 ± 0.052

3.825 ± 0.07

5.557 ± 0.128

4.16 ± 0.113

5.153 ± 0.077

0.943 ± 0.036

4.223 ± 0.078
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski