Arthrobacter phage Mudcat

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Mudcatvirus; Arthrobacter virus Mudcat

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140G702|A0A140G702_9CAUD Uncharacterized protein OS=Arthrobacter phage Mudcat OX=1796997 GN=70 PE=4 SV=1
MM1 pKa = 7.31GALATRR7 pKa = 11.84YY8 pKa = 8.05IHH10 pKa = 7.37KK11 pKa = 8.55PTEE14 pKa = 3.92VLAVEE19 pKa = 4.14LTEE22 pKa = 5.8DD23 pKa = 3.56NLEE26 pKa = 4.41SVAGWVNGDD35 pKa = 3.06IFQNPITSTMQSFFRR50 pKa = 11.84IEE52 pKa = 4.06SRR54 pKa = 11.84DD55 pKa = 3.6SEE57 pKa = 4.38FMAEE61 pKa = 3.95VGDD64 pKa = 5.15IVVLDD69 pKa = 3.56NDD71 pKa = 4.38EE72 pKa = 4.37FFSMDD77 pKa = 3.44KK78 pKa = 9.36EE79 pKa = 4.66TFYY82 pKa = 11.35EE83 pKa = 4.42KK84 pKa = 10.88YY85 pKa = 8.85VTSEE89 pKa = 3.83STNINGVWLTNPFSGG104 pKa = 4.86

Molecular weight: 11.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140G6V8|A0A140G6V8_9CAUD Uncharacterized protein OS=Arthrobacter phage Mudcat OX=1796997 GN=25 PE=4 SV=1
MM1 pKa = 7.44TMRR4 pKa = 11.84FTIMKK9 pKa = 8.64TRR11 pKa = 11.84TTMVSLINKK20 pKa = 8.7ALQSFQAVYY29 pKa = 10.55LKK31 pKa = 10.4IAEE34 pKa = 4.13PRR36 pKa = 11.84NIRR39 pKa = 11.84ILLFLAYY46 pKa = 10.24LIFIIIGVNGLAHH59 pKa = 7.02PPQTYY64 pKa = 9.67EE65 pKa = 3.8RR66 pKa = 11.84ALGSLTLVIAMFSFVSFGALLGSIAVLPGIWWLEE100 pKa = 3.75RR101 pKa = 11.84SGLLSMGIGVLFYY114 pKa = 11.19VLILYY119 pKa = 7.45EE120 pKa = 4.15RR121 pKa = 11.84HH122 pKa = 6.16ASPFAMGIAFIALLVCAVRR141 pKa = 11.84WLEE144 pKa = 3.5IRR146 pKa = 11.84RR147 pKa = 11.84YY148 pKa = 9.51QLAPRR153 pKa = 11.84EE154 pKa = 4.36GG155 pKa = 3.36

Molecular weight: 17.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

18882

32

1492

198.8

22.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.241 ± 0.55

0.583 ± 0.104

6.297 ± 0.302

6.948 ± 0.497

3.919 ± 0.24

6.832 ± 0.442

1.885 ± 0.183

6.239 ± 0.285

6.504 ± 0.344

8.05 ± 0.311

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.727 ± 0.172

4.766 ± 0.161

3.882 ± 0.241

3.241 ± 0.154

5.211 ± 0.339

6.096 ± 0.291

6.361 ± 0.324

6.938 ± 0.223

1.515 ± 0.153

3.765 ± 0.314
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski