Amino acid dipepetide frequency for Treponema sp. J25

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.16AlaAla: 7.16 ± 0.101	0.82AlaCys: 0.82 ± 0.032	3.306AlaAsp: 3.306 ± 0.072	5.346AlaGlu: 5.346 ± 0.092	3.503AlaPhe: 3.503 ± 0.064	6.118AlaGly: 6.118 ± 0.096	1.49AlaHis: 1.49 ± 0.044	5.166AlaIle: 5.166 ± 0.085	3.085AlaLys: 3.085 ± 0.074	10.151AlaLeu: 10.151 ± 0.126	1.771AlaMet: 1.771 ± 0.05	2.056AlaAsn: 2.056 ± 0.054	3.297AlaPro: 3.297 ± 0.062	3.271AlaGln: 3.271 ± 0.067	4.922AlaArg: 4.922 ± 0.084	4.866AlaSer: 4.866 ± 0.081	3.826AlaThr: 3.826 ± 0.087	5.228AlaVal: 5.228 ± 0.076	0.893AlaTrp: 0.893 ± 0.029	2.722AlaTyr: 2.722 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	0.747CysAla: 0.747 ± 0.027	0.12CysCys: 0.12 ± 0.013	0.425CysAsp: 0.425 ± 0.019	0.507CysGlu: 0.507 ± 0.024	0.409CysPhe: 0.409 ± 0.019	0.895CysGly: 0.895 ± 0.032	0.213CysHis: 0.213 ± 0.016	0.683CysIle: 0.683 ± 0.032	0.394CysLys: 0.394 ± 0.021	0.805CysLeu: 0.805 ± 0.028	0.199CysMet: 0.199 ± 0.017	0.261CysAsn: 0.261 ± 0.016	0.517CysPro: 0.517 ± 0.03	0.302CysGln: 0.302 ± 0.016	0.598CysArg: 0.598 ± 0.029	0.63CysSer: 0.63 ± 0.028	0.603CysThr: 0.603 ± 0.027	0.592CysVal: 0.592 ± 0.029	0.124CysTrp: 0.124 ± 0.012	0.363CysTyr: 0.363 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	3.476AspAla: 3.476 ± 0.067	0.409AspCys: 0.409 ± 0.022	1.845AspAsp: 1.845 ± 0.052	3.262AspGlu: 3.262 ± 0.066	2.385AspPhe: 2.385 ± 0.049	3.489AspGly: 3.489 ± 0.065	0.751AspHis: 0.751 ± 0.027	3.353AspIle: 3.353 ± 0.07	2.003AspLys: 2.003 ± 0.051	4.834AspLeu: 4.834 ± 0.076	1.109AspMet: 1.109 ± 0.037	1.344AspAsn: 1.344 ± 0.043	3.155AspPro: 3.155 ± 0.058	1.346AspGln: 1.346 ± 0.04	3.054AspArg: 3.054 ± 0.059	2.421AspSer: 2.421 ± 0.054	2.668AspThr: 2.668 ± 0.064	3.022AspVal: 3.022 ± 0.049	0.672AspTrp: 0.672 ± 0.026	1.702AspTyr: 1.702 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	6.374GluAla: 6.374 ± 0.098	0.406GluCys: 0.406 ± 0.024	3.139GluAsp: 3.139 ± 0.06	6.613GluGlu: 6.613 ± 0.124	2.1GluPhe: 2.1 ± 0.051	5.974GluGly: 5.974 ± 0.085	1.201GluHis: 1.201 ± 0.037	4.748GluIle: 4.748 ± 0.08	5.283GluLys: 5.283 ± 0.087	7.151GluLeu: 7.151 ± 0.1	1.4GluMet: 1.4 ± 0.039	2.59GluAsn: 2.59 ± 0.062	2.367GluPro: 2.367 ± 0.058	3.146GluGln: 3.146 ± 0.064	5.133GluArg: 5.133 ± 0.092	3.229GluSer: 3.229 ± 0.06	3.649GluThr: 3.649 ± 0.072	4.315GluVal: 4.315 ± 0.077	0.852GluTrp: 0.852 ± 0.031	2.382GluTyr: 2.382 ± 0.055	0.0GluXaa: 0.0 ± 0.0
Phe	2.97PheAla: 2.97 ± 0.055	0.547PheCys: 0.547 ± 0.024	2.142PheAsp: 2.142 ± 0.047	2.461PheGlu: 2.461 ± 0.053	2.7PhePhe: 2.7 ± 0.076	2.891PheGly: 2.891 ± 0.062	0.78PheHis: 0.78 ± 0.027	3.044PheIle: 3.044 ± 0.063	1.98PheLys: 1.98 ± 0.05	4.839PheLeu: 4.839 ± 0.079	0.964PheMet: 0.964 ± 0.027	1.457PheAsn: 1.457 ± 0.045	2.389PhePro: 2.389 ± 0.059	1.482PheGln: 1.482 ± 0.043	2.25PheArg: 2.25 ± 0.052	3.838PheSer: 3.838 ± 0.072	2.536PheThr: 2.536 ± 0.055	2.959PheVal: 2.959 ± 0.061	0.66PheTrp: 0.66 ± 0.029	1.588PheTyr: 1.588 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	6.201GlyAla: 6.201 ± 0.103	0.799GlyCys: 0.799 ± 0.032	3.245GlyAsp: 3.245 ± 0.059	4.73GlyGlu: 4.73 ± 0.083	3.402GlyPhe: 3.402 ± 0.061	5.66GlyGly: 5.66 ± 0.1	1.161GlyHis: 1.161 ± 0.038	5.872GlyIle: 5.872 ± 0.087	5.056GlyLys: 5.056 ± 0.082	7.302GlyLeu: 7.302 ± 0.093	1.866GlyMet: 1.866 ± 0.049	2.771GlyAsn: 2.771 ± 0.061	3.026GlyPro: 3.026 ± 0.065	2.084GlyGln: 2.084 ± 0.045	4.057GlyArg: 4.057 ± 0.07	4.756GlySer: 4.756 ± 0.098	4.823GlyThr: 4.823 ± 0.11	4.882GlyVal: 4.882 ± 0.072	1.111GlyTrp: 1.111 ± 0.038	2.75GlyTyr: 2.75 ± 0.059	0.0GlyXaa: 0.0 ± 0.0
His	1.09HisAla: 1.09 ± 0.036	0.244HisCys: 0.244 ± 0.017	0.732HisAsp: 0.732 ± 0.028	1.101HisGlu: 1.101 ± 0.036	0.903HisPhe: 0.903 ± 0.031	1.309HisGly: 1.309 ± 0.042	0.621HisHis: 0.621 ± 0.027	1.346HisIle: 1.346 ± 0.041	0.691HisLys: 0.691 ± 0.028	2.089HisLeu: 2.089 ± 0.05	0.359HisMet: 0.359 ± 0.021	0.568HisAsn: 0.568 ± 0.027	1.294HisPro: 1.294 ± 0.037	0.75HisGln: 0.75 ± 0.029	1.375HisArg: 1.375 ± 0.043	1.001HisSer: 1.001 ± 0.032	0.937HisThr: 0.937 ± 0.034	0.948HisVal: 0.948 ± 0.032	0.266HisTrp: 0.266 ± 0.018	0.73HisTyr: 0.73 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.124IleAla: 5.124 ± 0.074	0.553IleCys: 0.553 ± 0.023	3.562IleAsp: 3.562 ± 0.061	4.967IleGlu: 4.967 ± 0.081	2.521IlePhe: 2.521 ± 0.053	4.638IleGly: 4.638 ± 0.08	1.426IleHis: 1.426 ± 0.043	4.259IleIle: 4.259 ± 0.077	3.261IleLys: 3.261 ± 0.063	7.239IleLeu: 7.239 ± 0.106	1.209IleMet: 1.209 ± 0.039	2.182IleAsn: 2.182 ± 0.058	4.129IlePro: 4.129 ± 0.066	3.115IleGln: 3.115 ± 0.06	4.068IleArg: 4.068 ± 0.067	3.936IleSer: 3.936 ± 0.079	3.697IleThr: 3.697 ± 0.066	4.133IleVal: 4.133 ± 0.075	0.618IleTrp: 0.618 ± 0.027	1.903IleTyr: 1.903 ± 0.048	0.0IleXaa: 0.0 ± 0.0
Lys	4.121LysAla: 4.121 ± 0.076	0.23LysCys: 0.23 ± 0.018	2.822LysAsp: 2.822 ± 0.055	5.11LysGlu: 5.11 ± 0.085	1.145LysPhe: 1.145 ± 0.035	4.031LysGly: 4.031 ± 0.07	0.762LysHis: 0.762 ± 0.03	3.521LysIle: 3.521 ± 0.07	4.479LysLys: 4.479 ± 0.079	4.057LysLeu: 4.057 ± 0.066	1.121LysMet: 1.121 ± 0.035	2.619LysAsn: 2.619 ± 0.061	2.042LysPro: 2.042 ± 0.05	1.77LysGln: 1.77 ± 0.052	3.026LysArg: 3.026 ± 0.063	2.359LysSer: 2.359 ± 0.055	3.163LysThr: 3.163 ± 0.069	3.028LysVal: 3.028 ± 0.062	0.457LysTrp: 0.457 ± 0.022	1.516LysTyr: 1.516 ± 0.045	0.0LysXaa: 0.0 ± 0.0
Leu	8.832LeuAla: 8.832 ± 0.111	1.145LeuCys: 1.145 ± 0.04	4.843LeuAsp: 4.843 ± 0.068	7.609LeuGlu: 7.609 ± 0.095	5.445LeuPhe: 5.445 ± 0.099	7.407LeuGly: 7.407 ± 0.109	1.895LeuHis: 1.895 ± 0.052	6.191LeuIle: 6.191 ± 0.087	5.102LeuLys: 5.102 ± 0.075	11.09LeuLeu: 11.09 ± 0.164	2.122LeuMet: 2.122 ± 0.053	2.959LeuAsn: 2.959 ± 0.065	5.312LeuPro: 5.312 ± 0.091	4.112LeuGln: 4.112 ± 0.081	6.233LeuArg: 6.233 ± 0.095	8.055LeuSer: 8.055 ± 0.109	4.968LeuThr: 4.968 ± 0.076	7.797LeuVal: 7.797 ± 0.114	1.47LeuTrp: 1.47 ± 0.041	3.886LeuTyr: 3.886 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	2.067MetAla: 2.067 ± 0.047	0.116MetCys: 0.116 ± 0.011	1.086MetAsp: 1.086 ± 0.032	1.562MetGlu: 1.562 ± 0.041	0.632MetPhe: 0.632 ± 0.028	1.852MetGly: 1.852 ± 0.047	0.278MetHis: 0.278 ± 0.015	1.267MetIle: 1.267 ± 0.039	1.543MetLys: 1.543 ± 0.04	1.753MetLeu: 1.753 ± 0.044	0.498MetMet: 0.498 ± 0.026	0.938MetAsn: 0.938 ± 0.033	0.894MetPro: 0.894 ± 0.031	0.682MetGln: 0.682 ± 0.026	0.975MetArg: 0.975 ± 0.033	1.019MetSer: 1.019 ± 0.036	1.037MetThr: 1.037 ± 0.032	1.872MetVal: 1.872 ± 0.044	0.158MetTrp: 0.158 ± 0.013	0.581MetTyr: 0.581 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	2.107AsnAla: 2.107 ± 0.061	0.286AsnCys: 0.286 ± 0.02	1.334AsnAsp: 1.334 ± 0.039	1.91AsnGlu: 1.91 ± 0.051	1.407AsnPhe: 1.407 ± 0.04	2.437AsnGly: 2.437 ± 0.065	0.629AsnHis: 0.629 ± 0.026	2.349AsnIle: 2.349 ± 0.055	1.654AsnLys: 1.654 ± 0.044	3.714AsnLeu: 3.714 ± 0.066	0.737AsnMet: 0.737 ± 0.024	1.282AsnAsn: 1.282 ± 0.048	2.347AsnPro: 2.347 ± 0.053	1.286AsnGln: 1.286 ± 0.038	2.242AsnArg: 2.242 ± 0.052	1.715AsnSer: 1.715 ± 0.048	1.743AsnThr: 1.743 ± 0.047	1.777AsnVal: 1.777 ± 0.043	0.5AsnTrp: 0.5 ± 0.025	1.289AsnTyr: 1.289 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	3.858ProAla: 3.858 ± 0.076	0.414ProCys: 0.414 ± 0.022	2.456ProAsp: 2.456 ± 0.054	4.161ProGlu: 4.161 ± 0.087	2.485ProPhe: 2.485 ± 0.053	3.579ProGly: 3.579 ± 0.067	1.011ProHis: 1.011 ± 0.037	2.656ProIle: 2.656 ± 0.059	1.59ProLys: 1.59 ± 0.052	5.825ProLeu: 5.825 ± 0.098	0.836ProMet: 0.836 ± 0.027	1.243ProAsn: 1.243 ± 0.041	2.364ProPro: 2.364 ± 0.069	1.989ProGln: 1.989 ± 0.05	2.577ProArg: 2.577 ± 0.056	3.263ProSer: 3.263 ± 0.062	2.209ProThr: 2.209 ± 0.052	3.666ProVal: 3.666 ± 0.06	0.782ProTrp: 0.782 ± 0.034	1.856ProTyr: 1.856 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	3.102GlnAla: 3.102 ± 0.061	0.255GlnCys: 0.255 ± 0.014	1.98GlnAsp: 1.98 ± 0.048	3.711GlnGlu: 3.711 ± 0.079	1.327GlnPhe: 1.327 ± 0.039	3.209GlnGly: 3.209 ± 0.064	0.68GlnHis: 0.68 ± 0.027	2.216GlnIle: 2.216 ± 0.046	2.806GlnLys: 2.806 ± 0.065	3.033GlnLeu: 3.033 ± 0.056	0.694GlnMet: 0.694 ± 0.027	1.525GlnAsn: 1.525 ± 0.046	1.144GlnPro: 1.144 ± 0.035	1.607GlnGln: 1.607 ± 0.049	2.729GlnArg: 2.729 ± 0.062	1.885GlnSer: 1.885 ± 0.055	1.645GlnThr: 1.645 ± 0.039	2.517GlnVal: 2.517 ± 0.051	0.572GlnTrp: 0.572 ± 0.026	1.323GlnTyr: 1.323 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	4.547ArgAla: 4.547 ± 0.078	0.637ArgCys: 0.637 ± 0.031	2.93ArgAsp: 2.93 ± 0.061	4.585ArgGlu: 4.585 ± 0.079	2.825ArgPhe: 2.825 ± 0.057	4.192ArgGly: 4.192 ± 0.081	1.111ArgHis: 1.111 ± 0.031	4.666ArgIle: 4.666 ± 0.085	2.936ArgLys: 2.936 ± 0.059	6.66ArgLeu: 6.66 ± 0.092	1.33ArgMet: 1.33 ± 0.04	1.99ArgAsn: 1.99 ± 0.051	2.543ArgPro: 2.543 ± 0.051	2.137ArgGln: 2.137 ± 0.047	4.013ArgArg: 4.013 ± 0.072	3.65ArgSer: 3.65 ± 0.06	2.684ArgThr: 2.684 ± 0.053	3.546ArgVal: 3.546 ± 0.06	1.029ArgTrp: 1.029 ± 0.034	2.59ArgTyr: 2.59 ± 0.058	0.0ArgXaa: 0.0 ± 0.0
Ser	4.15SerAla: 4.15 ± 0.087	0.724SerCys: 0.724 ± 0.031	2.464SerAsp: 2.464 ± 0.052	3.443SerGlu: 3.443 ± 0.066	3.508SerPhe: 3.508 ± 0.076	4.821SerGly: 4.821 ± 0.111	1.18SerHis: 1.18 ± 0.04	3.909SerIle: 3.909 ± 0.074	2.27SerLys: 2.27 ± 0.052	7.557SerLeu: 7.557 ± 0.106	1.278SerMet: 1.278 ± 0.031	1.532SerAsn: 1.532 ± 0.047	3.421SerPro: 3.421 ± 0.07	2.415SerGln: 2.415 ± 0.053	3.932SerArg: 3.932 ± 0.062	4.329SerSer: 4.329 ± 0.095	2.902SerThr: 2.902 ± 0.062	3.979SerVal: 3.979 ± 0.07	1.023SerTrp: 1.023 ± 0.039	2.42SerTyr: 2.42 ± 0.056	0.0SerXaa: 0.0 ± 0.0
Thr	4.3ThrAla: 4.3 ± 0.078	0.458ThrCys: 0.458 ± 0.019	2.389ThrAsp: 2.389 ± 0.063	3.283ThrGlu: 3.283 ± 0.058	2.271ThrPhe: 2.271 ± 0.053	4.399ThrGly: 4.399 ± 0.09	0.908ThrHis: 0.908 ± 0.031	4.162ThrIle: 4.162 ± 0.08	2.051ThrLys: 2.051 ± 0.052	5.85ThrLeu: 5.85 ± 0.082	1.074ThrMet: 1.074 ± 0.031	1.646ThrAsn: 1.646 ± 0.046	2.699ThrPro: 2.699 ± 0.051	1.591ThrGln: 1.591 ± 0.037	2.25ThrArg: 2.25 ± 0.054	3.03ThrSer: 3.03 ± 0.067	3.149ThrThr: 3.149 ± 0.089	4.169ThrVal: 4.169 ± 0.087	0.58ThrTrp: 0.58 ± 0.03	1.958ThrTyr: 1.958 ± 0.051	0.0ThrXaa: 0.0 ± 0.0
Val	5.479ValAla: 5.479 ± 0.089	0.682ValCys: 0.682 ± 0.03	3.175ValAsp: 3.175 ± 0.06	4.277ValGlu: 4.277 ± 0.07	3.134ValPhe: 3.134 ± 0.054	4.723ValGly: 4.723 ± 0.076	1.245ValHis: 1.245 ± 0.041	4.229ValIle: 4.229 ± 0.072	2.785ValLys: 2.785 ± 0.058	7.332ValLeu: 7.332 ± 0.092	1.316ValMet: 1.316 ± 0.043	1.924ValAsn: 1.924 ± 0.049	3.567ValPro: 3.567 ± 0.065	2.719ValGln: 2.719 ± 0.055	3.873ValArg: 3.873 ± 0.066	4.575ValSer: 4.575 ± 0.072	3.236ValThr: 3.236 ± 0.076	5.31ValVal: 5.31 ± 0.089	0.756ValTrp: 0.756 ± 0.035	2.384ValTyr: 2.384 ± 0.057	0.0ValXaa: 0.0 ± 0.0
Trp	0.946TrpAla: 0.946 ± 0.032	0.141TrpCys: 0.141 ± 0.013	0.725TrpAsp: 0.725 ± 0.027	0.989TrpGlu: 0.989 ± 0.032	0.617TrpPhe: 0.617 ± 0.028	1.029TrpGly: 1.029 ± 0.034	0.231TrpHis: 0.231 ± 0.016	0.88TrpIle: 0.88 ± 0.031	0.857TrpLys: 0.857 ± 0.031	1.22TrpLeu: 1.22 ± 0.032	0.308TrpMet: 0.308 ± 0.018	0.631TrpAsn: 0.631 ± 0.026	0.476TrpPro: 0.476 ± 0.023	0.593TrpGln: 0.593 ± 0.025	0.614TrpArg: 0.614 ± 0.023	0.722TrpSer: 0.722 ± 0.029	0.657TrpThr: 0.657 ± 0.029	0.816TrpVal: 0.816 ± 0.032	0.231TrpTrp: 0.231 ± 0.015	0.621TrpTyr: 0.621 ± 0.031	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.348TyrAla: 2.348 ± 0.044	0.4TyrCys: 0.4 ± 0.022	1.699TyrAsp: 1.699 ± 0.052	2.425TyrGlu: 2.425 ± 0.056	1.755TyrPhe: 1.755 ± 0.046	2.903TyrGly: 2.903 ± 0.059	0.78TyrHis: 0.78 ± 0.032	2.024TyrIle: 2.024 ± 0.046	1.553TyrLys: 1.553 ± 0.043	3.974TyrLeu: 3.974 ± 0.076	0.617TyrMet: 0.617 ± 0.025	1.232TyrAsn: 1.232 ± 0.039	1.833TyrPro: 1.833 ± 0.053	1.562TyrGln: 1.562 ± 0.042	2.68TyrArg: 2.68 ± 0.056	1.98TyrSer: 1.98 ± 0.042	2.085TyrThr: 2.085 ± 0.046	2.164TyrVal: 2.164 ± 0.05	0.547TyrTrp: 0.547 ± 0.028	1.501TyrTyr: 1.501 ± 0.045	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2661 proteins (953465 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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