Halosimplex carlsbadense 2-9-1

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Haloarculaceae; Halosimplex; Halosimplex carlsbadense

Average proteome isoelectric point is 4.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4458 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M0CJ66|M0CJ66_9EURY Cupin 2 barrel domain-containing protein OS=Halosimplex carlsbadense 2-9-1 OX=797114 GN=C475_16216 PE=4 SV=1
MM1 pKa = 7.23ATDD4 pKa = 3.56ITRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84AIKK12 pKa = 10.55ALGLAGATGLAGCSSGGDD30 pKa = 3.53STATSGDD37 pKa = 3.7GGSGGSDD44 pKa = 3.01TVRR47 pKa = 11.84AAFVYY52 pKa = 10.15NSEE55 pKa = 4.47VGDD58 pKa = 4.03QGWSWAHH65 pKa = 5.41DD66 pKa = 3.46QGRR69 pKa = 11.84QAVAEE74 pKa = 4.1QYY76 pKa = 10.9DD77 pKa = 3.9WLEE80 pKa = 3.84TGYY83 pKa = 9.11TEE85 pKa = 5.15AVAPADD91 pKa = 3.43SRR93 pKa = 11.84TVFEE97 pKa = 5.41QYY99 pKa = 10.9VQDD102 pKa = 4.85DD103 pKa = 3.86YY104 pKa = 11.95DD105 pKa = 3.59IVFGNTFGYY114 pKa = 8.89MDD116 pKa = 3.67TMVSVAEE123 pKa = 4.34EE124 pKa = 4.27SPDD127 pKa = 3.4TLFEE131 pKa = 4.49HH132 pKa = 7.12CSGYY136 pKa = 8.06QTSEE140 pKa = 3.38NLGRR144 pKa = 11.84YY145 pKa = 8.5FGRR148 pKa = 11.84MYY150 pKa = 9.85QARR153 pKa = 11.84YY154 pKa = 8.48LAGIAAGMLTEE165 pKa = 5.0ADD167 pKa = 3.53SLGYY171 pKa = 10.01VAALPIAEE179 pKa = 4.64VIRR182 pKa = 11.84GINAFTLGARR192 pKa = 11.84SVNPDD197 pKa = 2.49ATTEE201 pKa = 4.02VRR203 pKa = 11.84WTNTWYY209 pKa = 10.16DD210 pKa = 3.58PPTEE214 pKa = 3.98QEE216 pKa = 4.03AANALIDD223 pKa = 3.59QGVDD227 pKa = 3.2VMAQHH232 pKa = 6.47QDD234 pKa = 2.71SAAAVQAAADD244 pKa = 3.73ADD246 pKa = 3.66IWATGYY252 pKa = 8.9DD253 pKa = 3.77APMLEE258 pKa = 4.25QGGDD262 pKa = 3.44NYY264 pKa = 9.6VTSPIWHH271 pKa = 6.41WEE273 pKa = 3.79EE274 pKa = 4.54FYY276 pKa = 11.34GPTLEE281 pKa = 4.38AVRR284 pKa = 11.84DD285 pKa = 4.2GSWEE289 pKa = 3.82SDD291 pKa = 3.75FYY293 pKa = 11.73FEE295 pKa = 5.24GLEE298 pKa = 3.89SDD300 pKa = 4.56IIGLSEE306 pKa = 4.41WGPEE310 pKa = 3.97VPDD313 pKa = 3.89DD314 pKa = 3.85VKK316 pKa = 11.5SSVEE320 pKa = 4.08EE321 pKa = 3.64THH323 pKa = 6.84SAIEE327 pKa = 4.5SGDD330 pKa = 3.47ADD332 pKa = 3.31VWADD336 pKa = 4.13SKK338 pKa = 11.36FADD341 pKa = 4.78ADD343 pKa = 3.97DD344 pKa = 3.97ATLFQDD350 pKa = 2.83MGSYY354 pKa = 10.31VEE356 pKa = 4.41GVEE359 pKa = 4.47GSVPEE364 pKa = 4.09

Molecular weight: 39.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M0D1Y2|M0D1Y2_9EURY Uridine phosphorylase OS=Halosimplex carlsbadense 2-9-1 OX=797114 GN=C475_05145 PE=3 SV=1
MM1 pKa = 6.85YY2 pKa = 10.42AEE4 pKa = 4.82PARR7 pKa = 11.84KK8 pKa = 6.08TTAPSAVNSKK18 pKa = 10.14KK19 pKa = 10.36PNARR23 pKa = 11.84RR24 pKa = 11.84PGYY27 pKa = 10.39SPARR31 pKa = 11.84LAAVALARR39 pKa = 11.84RR40 pKa = 11.84FVEE43 pKa = 4.29VPMSVSIPPICEE55 pKa = 4.15AYY57 pKa = 10.38DD58 pKa = 3.51SGISSFDD65 pKa = 3.48GEE67 pKa = 4.8VFPSLATSVTTGTSTATTGVLLTNPEE93 pKa = 4.26TGPATPTVASSCRR106 pKa = 11.84SRR108 pKa = 11.84LSPANAAIRR117 pKa = 11.84RR118 pKa = 11.84PRR120 pKa = 11.84IWTSPVRR127 pKa = 11.84LIPALKK133 pKa = 9.92ISIASTVIVAGLEE146 pKa = 4.04NPEE149 pKa = 4.08IPSLGEE155 pKa = 3.69IPVHH159 pKa = 5.62GRR161 pKa = 11.84RR162 pKa = 11.84TTSVTMIAIAVTSIGSHH179 pKa = 5.18SVTNSAMAPRR189 pKa = 11.84TTSPTTTMSTVTPAGADD206 pKa = 3.4GCRR209 pKa = 11.84SVTEE213 pKa = 4.2TEE215 pKa = 3.83RR216 pKa = 11.84LTRR219 pKa = 3.93

Molecular weight: 22.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4458

0

4458

1310473

29

2791

294.0

31.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.636 ± 0.064

0.683 ± 0.012

8.912 ± 0.045

8.319 ± 0.055

3.222 ± 0.025

9.066 ± 0.042

1.887 ± 0.019

3.371 ± 0.03

1.496 ± 0.022

8.564 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.646 ± 0.018

2.15 ± 0.026

4.79 ± 0.027

2.304 ± 0.02

6.847 ± 0.042

5.448 ± 0.036

6.359 ± 0.036

9.418 ± 0.037

1.221 ± 0.016

2.656 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski