Frankia sp. Cc1.17

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Frankiales; Frankiaceae; Frankia; unclassified Frankia

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6324 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S1Q2U7|A0A1S1Q2U7_9ACTN Transcriptional regulator WhiB OS=Frankia sp. Cc1.17 OX=573497 GN=whiB PE=3 SV=1
MM1 pKa = 7.63LSGGFDD7 pKa = 3.03QTIGNEE13 pKa = 4.15FTVQAATSPGGTIVDD28 pKa = 3.92AATVDD33 pKa = 3.13AAVYY37 pKa = 10.02DD38 pKa = 4.24RR39 pKa = 11.84EE40 pKa = 4.43PGRR43 pKa = 11.84SGYY46 pKa = 11.75SMMVSVGSGSDD57 pKa = 3.21GNYY60 pKa = 8.64RR61 pKa = 11.84ACVHH65 pKa = 6.35PEE67 pKa = 3.4RR68 pKa = 11.84SGFTCTDD75 pKa = 3.17DD76 pKa = 4.78RR77 pKa = 11.84SFALKK82 pKa = 9.83GQVVASIGLSVLADD96 pKa = 4.39PGPGNAIGGGTIIDD110 pKa = 4.25ALPDD114 pKa = 3.49GTEE117 pKa = 3.92VTFACYY123 pKa = 9.93RR124 pKa = 11.84RR125 pKa = 11.84DD126 pKa = 3.55VPIPGPYY133 pKa = 8.34GTSDD137 pKa = 2.45TWVRR141 pKa = 11.84IEE143 pKa = 5.08SYY145 pKa = 10.67DD146 pKa = 3.69PPGDD150 pKa = 3.7PTPDD154 pKa = 2.82TGEE157 pKa = 4.01QINPGSIDD165 pKa = 3.14RR166 pKa = 11.84SGFVSDD172 pKa = 3.49SFLYY176 pKa = 10.26TGADD180 pKa = 3.25TATFAPPCDD189 pKa = 3.46

Molecular weight: 19.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S1QXW6|A0A1S1QXW6_9ACTN Uncharacterized protein OS=Frankia sp. Cc1.17 OX=573497 GN=CC117_15325 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84THH17 pKa = 5.69GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILSARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.23GRR40 pKa = 11.84NEE42 pKa = 3.43LSAA45 pKa = 4.93

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6324

0

6324

2187273

37

6738

345.9

36.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.196 ± 0.055

0.753 ± 0.008

6.151 ± 0.02

5.1 ± 0.029

2.66 ± 0.016

9.829 ± 0.033

2.175 ± 0.016

3.357 ± 0.019

1.262 ± 0.018

10.221 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.592 ± 0.012

1.623 ± 0.016

6.609 ± 0.032

2.605 ± 0.015

8.537 ± 0.034

5.201 ± 0.024

6.095 ± 0.028

8.678 ± 0.028

1.431 ± 0.012

1.839 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski