Jonesia denitrificans (strain ATCC 14870 / DSM 20603 / BCRC 15368 / CIP 55.134 / JCM 11481 / NBRC 15587 / NCTC 10816 / Prevot 55134) (Listeria denitrificans)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Jonesiaceae; Jonesia; Jonesia denitrificans

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2495 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7QZC1|C7QZC1_JONDD ATP synthase subunit alpha OS=Jonesia denitrificans (strain ATCC 14870 / DSM 20603 / BCRC 15368 / CIP 55.134 / JCM 11481 / NBRC 15587 / NCTC 10816 / Prevot 55134) OX=471856 GN=atpA PE=3 SV=1
MM1 pKa = 6.95NHH3 pKa = 5.75HH4 pKa = 6.59TATHH8 pKa = 5.32TQATRR13 pKa = 11.84RR14 pKa = 11.84PLTTTLTALTLTAALAGCVPGSSNEE39 pKa = 3.98DD40 pKa = 3.24NAQGEE45 pKa = 4.74TPSTVTVVVHH55 pKa = 7.28DD56 pKa = 4.16SFQMSEE62 pKa = 3.93EE63 pKa = 3.93QQADD67 pKa = 3.73FEE69 pKa = 4.63KK70 pKa = 10.55TSGYY74 pKa = 11.06DD75 pKa = 3.2LTIVTNGDD83 pKa = 2.76GGALVNSLILTKK95 pKa = 10.44DD96 pKa = 3.19APIADD101 pKa = 3.46AVFGVDD107 pKa = 2.9NTFASRR113 pKa = 11.84GISEE117 pKa = 4.79EE118 pKa = 3.79VFAPYY123 pKa = 9.74TSEE126 pKa = 4.13VASQTSRR133 pKa = 11.84DD134 pKa = 3.49LALPGDD140 pKa = 4.23DD141 pKa = 4.09TLTAIDD147 pKa = 4.99FGDD150 pKa = 3.29VCLNVDD156 pKa = 3.59PTWFEE161 pKa = 3.8NHH163 pKa = 6.31SLDD166 pKa = 5.04VPATLDD172 pKa = 3.75DD173 pKa = 4.67LADD176 pKa = 3.7PAYY179 pKa = 10.88RR180 pKa = 11.84NLTVVTNPATSSPGLSFLFATIGKK204 pKa = 9.13YY205 pKa = 10.71GEE207 pKa = 5.08DD208 pKa = 3.88GYY210 pKa = 11.56LDD212 pKa = 3.67YY213 pKa = 8.34WTQLKK218 pKa = 11.03DD219 pKa = 3.35NGLKK223 pKa = 9.97IVDD226 pKa = 3.86GWEE229 pKa = 3.39DD230 pKa = 3.24AYY232 pKa = 11.66YY233 pKa = 11.24VDD235 pKa = 5.62FTATGDD241 pKa = 3.5GDD243 pKa = 3.91RR244 pKa = 11.84PIALSYY250 pKa = 10.85ASSPAFTVTDD260 pKa = 4.39DD261 pKa = 3.96GSATTTSALLDD272 pKa = 3.36TCFRR276 pKa = 11.84QVEE279 pKa = 4.46YY280 pKa = 11.15AGVLHH285 pKa = 6.87GAANPEE291 pKa = 3.98GAQAFIDD298 pKa = 3.87YY299 pKa = 10.22LLSEE303 pKa = 5.57DD304 pKa = 4.22YY305 pKa = 9.65QTTIPDD311 pKa = 3.58AMYY314 pKa = 8.45MYY316 pKa = 10.21PVADD320 pKa = 3.61TVEE323 pKa = 5.4LPDD326 pKa = 3.69AWAQFAPVPTTPISLDD342 pKa = 3.33PDD344 pKa = 4.21TIARR348 pKa = 11.84MRR350 pKa = 11.84DD351 pKa = 2.82TWIKK355 pKa = 10.48DD356 pKa = 2.88WSAQIIGG363 pKa = 3.71

Molecular weight: 38.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7R573|C7R573_JONDD Regulatory protein ArsR OS=Jonesia denitrificans (strain ATCC 14870 / DSM 20603 / BCRC 15368 / CIP 55.134 / JCM 11481 / NBRC 15587 / NCTC 10816 / Prevot 55134) OX=471856 GN=Jden_0075 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.25VRR4 pKa = 11.84NSLKK8 pKa = 10.4SLKK11 pKa = 9.52NQPGSQIVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GRR24 pKa = 11.84VFVINKK30 pKa = 9.17KK31 pKa = 9.28NPRR34 pKa = 11.84LKK36 pKa = 10.61ARR38 pKa = 11.84QGG40 pKa = 3.23

Molecular weight: 4.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2495

0

2495

819653

30

2043

328.5

35.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.756 ± 0.062

0.631 ± 0.012

6.264 ± 0.044

5.214 ± 0.046

2.941 ± 0.028

8.16 ± 0.046

2.709 ± 0.034

4.826 ± 0.031

2.263 ± 0.035

9.484 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.019 ± 0.018

2.544 ± 0.032

5.372 ± 0.04

3.44 ± 0.026

6.522 ± 0.05

5.945 ± 0.04

7.425 ± 0.064

8.882 ± 0.059

1.476 ± 0.021

2.124 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski