White clover mosaic virus (strain O) (WCMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus; White clover mosaic virus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P15406|CAPSD_WCMVO Coat protein OS=White clover mosaic virus (strain O) OX=12190 PE=3 SV=1
MM1 pKa = 8.18DD2 pKa = 4.32FTTLVIIGVYY12 pKa = 9.88LLVFIVYY19 pKa = 8.66FAKK22 pKa = 10.51INTSMCTISISGASVEE38 pKa = 5.58ISGCDD43 pKa = 3.23NPALFEE49 pKa = 4.3ILPNLKK55 pKa = 10.23PFDD58 pKa = 4.61HH59 pKa = 6.68GLSVPSII66 pKa = 3.9

Molecular weight: 7.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P15405|TGB3_WCMVO Movement protein TGBp3 OS=White clover mosaic virus (strain O) OX=12190 GN=ORF4 PE=3 SV=1
MM1 pKa = 7.63PLIPPPNPQKK11 pKa = 9.67TYY13 pKa = 10.67QIAVLALGLVLLLAFVLISDD33 pKa = 4.73HH34 pKa = 5.99SPKK37 pKa = 10.84VGDD40 pKa = 4.33HH41 pKa = 5.93LHH43 pKa = 6.12NLPFGGEE50 pKa = 4.17YY51 pKa = 10.26KK52 pKa = 10.79DD53 pKa = 3.44GTKK56 pKa = 9.75TIKK59 pKa = 10.42YY60 pKa = 6.97FQRR63 pKa = 11.84PNQHH67 pKa = 6.21SLSKK71 pKa = 9.83TLAKK75 pKa = 10.12SHH77 pKa = 5.92NTTIFLIILGLIGTLHH93 pKa = 6.22GLHH96 pKa = 6.13YY97 pKa = 10.42FSNNRR102 pKa = 11.84RR103 pKa = 11.84ISSSLHH109 pKa = 6.48CVLCQNKK116 pKa = 9.2HH117 pKa = 4.36

Molecular weight: 13.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1921

66

1294

384.2

43.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.184 ± 1.215

1.51 ± 0.129

5.362 ± 0.548

5.206 ± 1.139

5.102 ± 0.49

4.321 ± 0.784

3.54 ± 0.665

7.496 ± 0.521

6.143 ± 0.77

10.255 ± 1.196

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.718 ± 0.342

5.206 ± 0.736

6.247 ± 0.401

3.904 ± 0.424

3.644 ± 0.425

6.715 ± 0.806

7.496 ± 0.424

4.425 ± 0.47

1.093 ± 0.31

3.436 ± 0.307
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski