Sida mottle virus-[Brazil]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Sida mottle virus

Average proteome isoelectric point is 7.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8QMH8|Q8QMH8_9GEMI Capsid protein OS=Sida mottle virus-[Brazil] OX=223319 GN=AV1 PE=3 SV=1
MM1 pKa = 7.49IVDD4 pKa = 4.59LPKK7 pKa = 10.73PLDD10 pKa = 3.49QRR12 pKa = 11.84LLIAGILSTRR22 pKa = 11.84DD23 pKa = 3.11LGIEE27 pKa = 4.04LVHH30 pKa = 7.67DD31 pKa = 4.79LEE33 pKa = 5.16TITEE37 pKa = 4.2IVLHH41 pKa = 6.25GCSAGLVVKK50 pKa = 10.38HH51 pKa = 5.66VEE53 pKa = 4.09HH54 pKa = 6.65LAEE57 pKa = 4.17IHH59 pKa = 5.42WCAIRR64 pKa = 11.84STVPDD69 pKa = 3.71QPEE72 pKa = 3.9HH73 pKa = 5.15ATVRR77 pKa = 11.84VVLEE81 pKa = 4.13LDD83 pKa = 3.68ILVHH87 pKa = 6.93PYY89 pKa = 8.71LTT91 pKa = 3.88

Molecular weight: 10.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8QMH8|Q8QMH8_9GEMI Capsid protein OS=Sida mottle virus-[Brazil] OX=223319 GN=AV1 PE=3 SV=1
MM1 pKa = 7.94PKK3 pKa = 9.54RR4 pKa = 11.84DD5 pKa = 3.67PSWRR9 pKa = 11.84QMAGTSKK16 pKa = 10.57VSRR19 pKa = 11.84SSNFSPRR26 pKa = 11.84GGIGPKK32 pKa = 9.75FNKK35 pKa = 9.8ASEE38 pKa = 4.21WVNRR42 pKa = 11.84PMYY45 pKa = 9.79RR46 pKa = 11.84KK47 pKa = 8.89PRR49 pKa = 11.84IYY51 pKa = 9.77RR52 pKa = 11.84TIRR55 pKa = 11.84TPDD58 pKa = 3.15VPRR61 pKa = 11.84GCEE64 pKa = 4.34GPCKK68 pKa = 9.32VQSYY72 pKa = 6.94EE73 pKa = 3.74QRR75 pKa = 11.84HH76 pKa = 6.38DD77 pKa = 3.16ISHH80 pKa = 6.13VGKK83 pKa = 10.55VMCISDD89 pKa = 3.56VTRR92 pKa = 11.84GNGITHH98 pKa = 6.78RR99 pKa = 11.84VGKK102 pKa = 9.44RR103 pKa = 11.84FCVKK107 pKa = 9.92SVYY110 pKa = 10.16ILGKK114 pKa = 9.37IWMDD118 pKa = 3.39EE119 pKa = 4.0NIKK122 pKa = 10.61LKK124 pKa = 10.79NHH126 pKa = 6.24TNSCMFWLVRR136 pKa = 11.84DD137 pKa = 3.74RR138 pKa = 11.84RR139 pKa = 11.84PYY141 pKa = 8.05GTPMDD146 pKa = 4.6FGQVFNMFDD155 pKa = 3.99NEE157 pKa = 4.11PSTATVKK164 pKa = 10.85NDD166 pKa = 2.82LRR168 pKa = 11.84DD169 pKa = 3.54RR170 pKa = 11.84FQVMHH175 pKa = 6.97KK176 pKa = 9.73FYY178 pKa = 11.33AKK180 pKa = 9.12VTGGQYY186 pKa = 11.1ASNEE190 pKa = 3.73QALVKK195 pKa = 10.5RR196 pKa = 11.84FWKK199 pKa = 10.3VNNHH203 pKa = 4.48VVYY206 pKa = 10.67NHH208 pKa = 5.8QEE210 pKa = 3.47AGKK213 pKa = 10.41YY214 pKa = 8.39EE215 pKa = 4.09NHH217 pKa = 6.52TEE219 pKa = 3.99NALLLYY225 pKa = 7.29MACTHH230 pKa = 7.07ASNPVYY236 pKa = 9.96ATLQIRR242 pKa = 11.84IYY244 pKa = 10.1FYY246 pKa = 11.33EE247 pKa = 4.8SITNN251 pKa = 3.61

Molecular weight: 29.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1006

43

360

167.7

19.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.274 ± 0.59

1.889 ± 0.682

4.771 ± 0.772

5.268 ± 0.85

3.877 ± 0.746

5.666 ± 0.444

4.076 ± 0.482

5.765 ± 0.615

5.567 ± 0.913

8.748 ± 1.904

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.187 ± 0.605

5.169 ± 0.82

5.765 ± 1.063

4.672 ± 0.723

7.058 ± 0.929

7.058 ± 1.469

5.865 ± 1.059

6.461 ± 1.159

1.988 ± 0.265

3.877 ± 0.89
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski