Escherichia phage vB_Eco_mar001J1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tempevirinae; Warwickvirus; Escherichia virus mar001J1

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P4A887|A0A3P4A887_9CAUD Uncharacterized protein OS=Escherichia phage vB_Eco_mar001J1 OX=2419760 GN=MAR001J1_00035 PE=4 SV=1
MM1 pKa = 7.0SQDD4 pKa = 3.09GFFEE8 pKa = 4.19RR9 pKa = 11.84LQMAEE14 pKa = 4.03DD15 pKa = 3.5SGLNKK20 pKa = 9.62EE21 pKa = 4.35AALEE25 pKa = 3.86VAYY28 pKa = 9.62KK29 pKa = 10.45IKK31 pKa = 9.82TLDD34 pKa = 3.49EE35 pKa = 4.55ALGDD39 pKa = 3.36MDD41 pKa = 5.18MDD43 pKa = 4.64LEE45 pKa = 4.42SSAIFADD52 pKa = 3.21PTMIVNDD59 pKa = 4.79CGCDD63 pKa = 3.74FDD65 pKa = 5.3PGCPRR70 pKa = 11.84CFPFF74 pKa = 5.95

Molecular weight: 8.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P4A812|A0A3P4A812_9CAUD Uncharacterized protein OS=Escherichia phage vB_Eco_mar001J1 OX=2419760 GN=MAR001J1_00040 PE=4 SV=1
MM1 pKa = 7.58VNIRR5 pKa = 11.84KK6 pKa = 8.9IRR8 pKa = 11.84VGTKK12 pKa = 9.82FLVTYY17 pKa = 8.72AIPEE21 pKa = 4.1SGMKK25 pKa = 9.91KK26 pKa = 9.51GQIVKK31 pKa = 9.91VDD33 pKa = 3.41SKK35 pKa = 11.27RR36 pKa = 11.84FGIHH40 pKa = 7.14GINGYY45 pKa = 10.07LINRR49 pKa = 11.84DD50 pKa = 3.31LGFDD54 pKa = 4.04GFAIRR59 pKa = 11.84ALEE62 pKa = 4.64GIVATLKK69 pKa = 10.63RR70 pKa = 11.84ITNHH74 pKa = 6.15RR75 pKa = 11.84GCPIKK80 pKa = 9.86TIKK83 pKa = 10.97APVNYY88 pKa = 9.97GKK90 pKa = 10.68LDD92 pKa = 3.29NRR94 pKa = 11.84RR95 pKa = 11.84IRR97 pKa = 11.84RR98 pKa = 11.84LARR101 pKa = 11.84NALKK105 pKa = 10.48FRR107 pKa = 11.84GVGDD111 pKa = 3.69DD112 pKa = 4.27FYY114 pKa = 11.7YY115 pKa = 10.42GYY117 pKa = 11.16KK118 pKa = 10.33RR119 pKa = 11.84IARR122 pKa = 11.84NAGKK126 pKa = 10.23

Molecular weight: 14.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

15141

42

1192

191.7

21.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.813 ± 0.376

1.38 ± 0.162

6.314 ± 0.274

6.677 ± 0.253

4.016 ± 0.164

7.556 ± 0.243

1.896 ± 0.251

6.499 ± 0.197

7.476 ± 0.35

6.354 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.012 ± 0.195

4.848 ± 0.183

3.256 ± 0.202

3.93 ± 0.283

5.284 ± 0.282

6.017 ± 0.212

4.927 ± 0.24

7.265 ± 0.296

1.585 ± 0.108

3.897 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski