Wheat dwarf virus (isolate Sweden) (WDV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus; Wheat dwarf virus

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P06849|MP_WDVS Movement protein OS=Wheat dwarf virus (isolate Sweden) OX=268789 GN=V2 PE=3 SV=1
MM1 pKa = 7.66ASSSAPRR8 pKa = 11.84FRR10 pKa = 11.84VYY12 pKa = 10.78SKK14 pKa = 11.3YY15 pKa = 11.01LFLTYY20 pKa = 9.26PEE22 pKa = 4.63CTLEE26 pKa = 3.85PQYY29 pKa = 11.83ALDD32 pKa = 4.26SLRR35 pKa = 11.84TLLNKK40 pKa = 10.04YY41 pKa = 9.36EE42 pKa = 4.24PLYY45 pKa = 10.1IAAVRR50 pKa = 11.84EE51 pKa = 4.02LHH53 pKa = 6.85EE54 pKa = 5.61DD55 pKa = 4.13GSPHH59 pKa = 6.69LHH61 pKa = 5.96VLVQNKK67 pKa = 8.63LRR69 pKa = 11.84ASITNPNALNLRR81 pKa = 11.84MDD83 pKa = 4.13TSPFSIFHH91 pKa = 6.53PNIQAAKK98 pKa = 9.81DD99 pKa = 3.72CNQVRR104 pKa = 11.84DD105 pKa = 4.27YY106 pKa = 9.75ITKK109 pKa = 10.41EE110 pKa = 3.51VDD112 pKa = 3.0SDD114 pKa = 4.11VNTAEE119 pKa = 3.73WGTFVAVSTPGRR131 pKa = 11.84KK132 pKa = 9.51DD133 pKa = 2.79RR134 pKa = 11.84DD135 pKa = 3.22ADD137 pKa = 3.4MKK139 pKa = 10.91QIIEE143 pKa = 4.28SSSSRR148 pKa = 11.84EE149 pKa = 3.73EE150 pKa = 4.01FLSMVCNRR158 pKa = 11.84FPFEE162 pKa = 3.45WSIRR166 pKa = 11.84LKK168 pKa = 10.83DD169 pKa = 3.77FEE171 pKa = 4.34YY172 pKa = 10.01TARR175 pKa = 11.84HH176 pKa = 6.38LFPDD180 pKa = 4.45PVATYY185 pKa = 9.18TPEE188 pKa = 4.05FPTEE192 pKa = 3.81SLICHH197 pKa = 5.52EE198 pKa = 5.27TIEE201 pKa = 4.35SWKK204 pKa = 10.62NEE206 pKa = 3.76HH207 pKa = 6.69LYY209 pKa = 10.78SVSLEE214 pKa = 4.09SYY216 pKa = 9.72ILCTSTPADD225 pKa = 3.44QAQSDD230 pKa = 4.74LEE232 pKa = 4.23WMDD235 pKa = 4.06DD236 pKa = 3.49YY237 pKa = 11.8SRR239 pKa = 11.84SHH241 pKa = 7.0RR242 pKa = 11.84GGISPSTSAGQPEE255 pKa = 4.43QEE257 pKa = 4.2RR258 pKa = 11.84LPGQGLL264 pKa = 3.44

Molecular weight: 30.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q67622|REP_WDVS Replication-associated protein OS=Wheat dwarf virus (isolate Sweden) OX=268789 GN=C1/C2 PE=1 SV=2
MM1 pKa = 6.79VTNKK5 pKa = 10.32DD6 pKa = 3.24SRR8 pKa = 11.84GKK10 pKa = 10.42GKK12 pKa = 10.62RR13 pKa = 11.84KK14 pKa = 8.95MEE16 pKa = 4.43EE17 pKa = 4.38GEE19 pKa = 4.34SSGRR23 pKa = 11.84WKK25 pKa = 10.56GAVYY29 pKa = 10.24KK30 pKa = 10.38RR31 pKa = 11.84RR32 pKa = 11.84KK33 pKa = 7.79QAYY36 pKa = 8.07KK37 pKa = 10.14VVPVKK42 pKa = 10.61PPALCVFRR50 pKa = 11.84YY51 pKa = 9.52NWLNSDD57 pKa = 3.32RR58 pKa = 11.84TNIVVGNTPRR68 pKa = 11.84VDD70 pKa = 5.54LITCFAQGKK79 pKa = 9.49ADD81 pKa = 3.94NNRR84 pKa = 11.84HH85 pKa = 4.81TNQTVLYY92 pKa = 8.93KK93 pKa = 10.21FNIQGTCYY101 pKa = 9.88MSDD104 pKa = 2.84ASAPFIGPVRR114 pKa = 11.84LYY116 pKa = 10.41HH117 pKa = 5.83WLVYY121 pKa = 10.28DD122 pKa = 4.74AEE124 pKa = 4.87PKK126 pKa = 10.12QAMPDD131 pKa = 3.21ATDD134 pKa = 3.14IFTMPWNLLPSTWTVQRR151 pKa = 11.84AWSHH155 pKa = 5.48RR156 pKa = 11.84FVVKK160 pKa = 10.5RR161 pKa = 11.84KK162 pKa = 5.39WTVNLVTDD170 pKa = 4.01GRR172 pKa = 11.84KK173 pKa = 9.77VGSKK177 pKa = 9.13TVDD180 pKa = 2.98QRR182 pKa = 11.84YY183 pKa = 8.54NWVVGKK189 pKa = 10.44NIVDD193 pKa = 3.38ANKK196 pKa = 9.44FFKK199 pKa = 10.54GLRR202 pKa = 11.84VTTEE206 pKa = 3.21WMNTGDD212 pKa = 5.0GKK214 pKa = 10.85IGDD217 pKa = 3.88IKK219 pKa = 10.84KK220 pKa = 10.23GALYY224 pKa = 10.24LISSTRR230 pKa = 11.84GGVTGDD236 pKa = 3.47SASTAFDD243 pKa = 3.61VVCAYY248 pKa = 8.43THH250 pKa = 6.31ACYY253 pKa = 10.13FKK255 pKa = 10.94AIGIQQ260 pKa = 3.17

Molecular weight: 29.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

965

90

351

241.3

27.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.425 ± 0.477

1.865 ± 0.094

5.907 ± 0.212

5.389 ± 1.205

4.456 ± 0.313

5.078 ± 1.099

2.28 ± 0.426

5.078 ± 0.402

5.596 ± 1.009

7.15 ± 0.925

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.969 ± 0.124

4.767 ± 0.503

5.907 ± 0.606

3.316 ± 0.539

6.01 ± 0.272

7.772 ± 1.255

7.254 ± 0.309

6.528 ± 1.053

2.176 ± 0.402

5.078 ± 0.444
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski