Beihai tombus-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 9.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFQ7|A0A1L3KFQ7_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 2 OX=1922723 PE=4 SV=1
MM1 pKa = 7.56SISDD5 pKa = 3.51VLQIQKK11 pKa = 10.73GVTCLSATIPRR22 pKa = 11.84QVRR25 pKa = 11.84RR26 pKa = 11.84LPRR29 pKa = 11.84VFYY32 pKa = 10.29TIAYY36 pKa = 8.24ANNFRR41 pKa = 11.84IPNNDD46 pKa = 3.51LVTKK50 pKa = 9.62LHH52 pKa = 6.29AFVEE56 pKa = 4.96RR57 pKa = 11.84VCLVRR62 pKa = 11.84NSEE65 pKa = 4.29GNLVATPRR73 pKa = 11.84PWDD76 pKa = 3.36VLMSEE81 pKa = 5.2DD82 pKa = 3.56VDD84 pKa = 3.9YY85 pKa = 11.51DD86 pKa = 3.43QAEE89 pKa = 4.02QLFIDD94 pKa = 3.7MTAPVVEE101 pKa = 4.3ALSRR105 pKa = 11.84EE106 pKa = 4.49YY107 pKa = 9.43ITAGGVDD114 pKa = 3.53QPITYY119 pKa = 10.03DD120 pKa = 3.07QFLNHH125 pKa = 5.86YY126 pKa = 8.95VGSKK130 pKa = 9.94RR131 pKa = 11.84KK132 pKa = 9.54IYY134 pKa = 9.44EE135 pKa = 3.64QAIEE139 pKa = 4.25SLRR142 pKa = 11.84DD143 pKa = 3.09IPFQDD148 pKa = 3.3KK149 pKa = 10.64RR150 pKa = 11.84DD151 pKa = 3.57GSVEE155 pKa = 3.78IFIKK159 pKa = 10.57PEE161 pKa = 3.62YY162 pKa = 9.19LKK164 pKa = 10.77PGGVPRR170 pKa = 11.84VISPRR175 pKa = 11.84HH176 pKa = 5.04PRR178 pKa = 11.84YY179 pKa = 9.22HH180 pKa = 5.78ATIGLYY186 pKa = 9.86IKK188 pKa = 10.52AIEE191 pKa = 4.1DD192 pKa = 3.67KK193 pKa = 10.15IYY195 pKa = 11.33NSIDD199 pKa = 3.64TMWDD203 pKa = 2.9PTGQFKK209 pKa = 9.02TVAKK213 pKa = 9.93KK214 pKa = 9.86MDD216 pKa = 3.89MIQRR220 pKa = 11.84AEE222 pKa = 4.44EE223 pKa = 4.06LNKK226 pKa = 9.23MWSSYY231 pKa = 9.58GRR233 pKa = 11.84PRR235 pKa = 11.84AVGFDD240 pKa = 3.24AKK242 pKa = 10.8RR243 pKa = 11.84FDD245 pKa = 3.32QHH247 pKa = 6.72INSWLLKK254 pKa = 9.27HH255 pKa = 6.59LEE257 pKa = 3.78HH258 pKa = 7.4KK259 pKa = 10.16IYY261 pKa = 10.91KK262 pKa = 9.1KK263 pKa = 10.11VCHH266 pKa = 5.59NTSPEE271 pKa = 4.08HH272 pKa = 6.18EE273 pKa = 4.48VTLSRR278 pKa = 11.84LLEE281 pKa = 4.09LQQTNIIKK289 pKa = 10.8VGDD292 pKa = 3.89RR293 pKa = 11.84KK294 pKa = 11.2DD295 pKa = 3.14MARR298 pKa = 11.84YY299 pKa = 6.8TFEE302 pKa = 3.87VDD304 pKa = 3.3GVRR307 pKa = 11.84MSGDD311 pKa = 3.33MNTSLGNVTIMCCLMWLFRR330 pKa = 11.84EE331 pKa = 4.77EE332 pKa = 3.9YY333 pKa = 10.6HH334 pKa = 7.44FDD336 pKa = 3.55FKK338 pKa = 10.91MLNDD342 pKa = 4.02GDD344 pKa = 4.67DD345 pKa = 3.77LVIVCDD351 pKa = 3.56RR352 pKa = 11.84VTAKK356 pKa = 10.53AIKK359 pKa = 10.18DD360 pKa = 3.5KK361 pKa = 11.23LPSFFLKK368 pKa = 10.67FGIEE372 pKa = 3.98MEE374 pKa = 4.34FEE376 pKa = 4.47GLFHH380 pKa = 6.38TLEE383 pKa = 5.22AIEE386 pKa = 4.71FCQAHH391 pKa = 6.21PVQYY395 pKa = 10.91DD396 pKa = 3.25VGKK399 pKa = 9.71WIMVPNPSKK408 pKa = 10.93RR409 pKa = 11.84IFSDD413 pKa = 3.64LLSEE417 pKa = 4.65KK418 pKa = 9.98PVEE421 pKa = 4.06SRR423 pKa = 11.84KK424 pKa = 9.88IYY426 pKa = 10.41SSWLGAVGKK435 pKa = 9.23CGCAMSPGVPILQEE449 pKa = 4.06FYY451 pKa = 11.23DD452 pKa = 4.22WILRR456 pKa = 11.84TPGVKK461 pKa = 9.54PWIPKK466 pKa = 9.18EE467 pKa = 3.34GSYY470 pKa = 10.34YY471 pKa = 10.55YY472 pKa = 10.61KK473 pKa = 9.98YY474 pKa = 10.74GYY476 pKa = 10.11RR477 pKa = 11.84KK478 pKa = 9.88VIEE481 pKa = 4.14GAKK484 pKa = 9.08YY485 pKa = 10.33KK486 pKa = 10.12PVSEE490 pKa = 4.15IARR493 pKa = 11.84ISFFKK498 pKa = 10.5AYY500 pKa = 9.96NIPPSEE506 pKa = 4.02QIMLEE511 pKa = 3.85NMFRR515 pKa = 11.84SLPSPSRR522 pKa = 11.84EE523 pKa = 3.6ILEE526 pKa = 4.26EE527 pKa = 3.94SSRR530 pKa = 11.84PAHH533 pKa = 5.55TLPLEE538 pKa = 4.23MFALPTYY545 pKa = 9.97FKK547 pKa = 10.71KK548 pKa = 10.98FNWEE552 pKa = 3.38EE553 pKa = 3.74TDD555 pKa = 4.36RR556 pKa = 11.84MNRR559 pKa = 11.84WEE561 pKa = 4.28LDD563 pKa = 2.93RR564 pKa = 11.84GLFLVKK570 pKa = 10.26NKK572 pKa = 9.51RR573 pKa = 11.84RR574 pKa = 11.84AQDD577 pKa = 3.06KK578 pKa = 10.42GKK580 pKa = 11.32SNLTVKK586 pKa = 10.1NVKK589 pKa = 9.72KK590 pKa = 10.75DD591 pKa = 3.85PIRR594 pKa = 11.84FRR596 pKa = 11.84KK597 pKa = 9.02PKK599 pKa = 9.08RR600 pKa = 11.84SQRR603 pKa = 11.84RR604 pKa = 11.84RR605 pKa = 11.84ARR607 pKa = 11.84YY608 pKa = 6.61TSKK611 pKa = 10.35HH612 pKa = 5.26CRR614 pKa = 11.84HH615 pKa = 6.48RR616 pKa = 11.84SAKK619 pKa = 8.8TRR621 pKa = 11.84SAVTNLTKK629 pKa = 10.4SSRR632 pKa = 11.84QKK634 pKa = 10.32RR635 pKa = 11.84RR636 pKa = 11.84RR637 pKa = 11.84SAYY640 pKa = 8.48RR641 pKa = 11.84FRR643 pKa = 11.84YY644 pKa = 9.59RR645 pKa = 11.84SAA647 pKa = 3.46

Molecular weight: 75.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFQ7|A0A1L3KFQ7_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 2 OX=1922723 PE=4 SV=1
MM1 pKa = 7.47EE2 pKa = 4.35KK3 pKa = 10.38VIRR6 pKa = 11.84KK7 pKa = 4.91VTHH10 pKa = 5.93VIVSQRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84CAKK21 pKa = 10.33VIAQFTGRR29 pKa = 11.84CFRR32 pKa = 11.84CLAYY36 pKa = 10.38RR37 pKa = 11.84ATQLTQCRR45 pKa = 11.84VKK47 pKa = 10.43VIRR50 pKa = 11.84GAVVGTLKK58 pKa = 10.43RR59 pKa = 11.84VKK61 pKa = 9.76GQSRR65 pKa = 11.84RR66 pKa = 11.84LLVYY70 pKa = 10.32KK71 pKa = 10.32IRR73 pKa = 11.84NACRR77 pKa = 11.84GDD79 pKa = 3.32LKK81 pKa = 11.21LRR83 pKa = 11.84FQGTYY88 pKa = 9.92KK89 pKa = 10.54AVEE92 pKa = 4.5TIVDD96 pKa = 3.86RR97 pKa = 11.84TGHH100 pKa = 6.58AIEE103 pKa = 5.92IILGWMLQIFLAWFALFLMYY123 pKa = 10.88KK124 pKa = 10.33LGVLMNHH131 pKa = 7.11IYY133 pKa = 10.9LNYY136 pKa = 9.72IKK138 pKa = 10.37PEE140 pKa = 4.09YY141 pKa = 10.17EE142 pKa = 4.59SIATDD147 pKa = 3.15IPTKK151 pKa = 9.56MKK153 pKa = 9.76EE154 pKa = 3.97RR155 pKa = 11.84QRR157 pKa = 11.84LVYY160 pKa = 10.52CGMTWRR166 pKa = 11.84RR167 pKa = 11.84KK168 pKa = 8.62LMHH171 pKa = 6.51WRR173 pKa = 11.84MQLARR178 pKa = 11.84FFKK181 pKa = 10.53RR182 pKa = 11.84PVSGVEE188 pKa = 3.81PTPVTEE194 pKa = 4.13QIKK197 pKa = 10.22VLEE200 pKa = 4.38ALFEE204 pKa = 4.93ADD206 pKa = 4.18QLHH209 pKa = 6.56EE210 pKa = 4.93LPPLCTFRR218 pKa = 11.84GKK220 pKa = 9.96IVFSYY225 pKa = 10.96HH226 pKa = 6.43LAFKK230 pKa = 10.12MKK232 pKa = 9.49ATLGVVGKK240 pKa = 10.78YY241 pKa = 10.0DD242 pKa = 3.42VARR245 pKa = 11.84TNVLIRR251 pKa = 11.84TANAILVEE259 pKa = 4.23MLQEE263 pKa = 3.96MSDD266 pKa = 3.72EE267 pKa = 4.07EE268 pKa = 4.4KK269 pKa = 11.05EE270 pKa = 4.07RR271 pKa = 11.84VKK273 pKa = 10.74LLKK276 pKa = 10.28YY277 pKa = 10.45NHH279 pKa = 6.19GLRR282 pKa = 11.84HH283 pKa = 5.71KK284 pKa = 9.5VVCQAIAIFKK294 pKa = 10.17IPSFDD299 pKa = 3.71EE300 pKa = 3.84VSMAEE305 pKa = 4.14DD306 pKa = 3.34LRR308 pKa = 11.84ASVLSQITDD317 pKa = 3.24AFVSKK322 pKa = 11.0VGGPSNN328 pKa = 3.55

Molecular weight: 37.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

975

328

647

487.5

56.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.949 ± 0.761

1.846 ± 0.33

4.513 ± 0.984

5.949 ± 0.256

4.718 ± 0.081

4.821 ± 0.202

2.359 ± 0.045

6.564 ± 0.08

8.0 ± 0.041

8.205 ± 1.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.282 ± 0.21

3.179 ± 0.582

4.615 ± 1.041

3.59 ± 0.378

8.615 ± 0.126

5.744 ± 1.16

4.821 ± 0.371

7.59 ± 1.206

1.538 ± 0.177

4.103 ± 0.417
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski