Botrytis ourmia-like virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Miaviricetes; Ourlivirales; Botourmiaviridae; Botoulivirus; Botrytis botoulivirus

Average proteome isoelectric point is 8.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S4GA80|A0A0S4GA80_9VIRU RNA dependent RNA polymerase OS=Botrytis ourmia-like virus OX=1872719 GN=RdRp PE=4 SV=1
MM1 pKa = 7.22PTSNDD6 pKa = 2.97MEE8 pKa = 4.97AGCKK12 pKa = 7.73ATRR15 pKa = 11.84SIRR18 pKa = 11.84EE19 pKa = 3.87SLLRR23 pKa = 11.84ATQLISRR30 pKa = 11.84EE31 pKa = 4.01FNLPVPVGPFNGTSCMEE48 pKa = 5.05LRR50 pKa = 11.84SQWDD54 pKa = 3.95TYY56 pKa = 11.65AKK58 pKa = 9.61WSCLQFVSKK67 pKa = 10.7KK68 pKa = 7.33EE69 pKa = 3.96EE70 pKa = 3.8RR71 pKa = 11.84RR72 pKa = 11.84KK73 pKa = 8.43TLVQLIKK80 pKa = 10.56SGCRR84 pKa = 11.84LFDD87 pKa = 4.07GDD89 pKa = 5.98CRR91 pKa = 11.84TCDD94 pKa = 3.43PKK96 pKa = 11.16LKK98 pKa = 10.23EE99 pKa = 3.84LEE101 pKa = 3.83KK102 pKa = 10.76RR103 pKa = 11.84KK104 pKa = 9.19WVEE107 pKa = 3.61QMSTARR113 pKa = 11.84PRR115 pKa = 11.84VAYY118 pKa = 10.27YY119 pKa = 10.52KK120 pKa = 10.87GLTRR124 pKa = 11.84STWLGRR130 pKa = 11.84LKK132 pKa = 10.9DD133 pKa = 3.65SARR136 pKa = 11.84EE137 pKa = 4.01LITGWGRR144 pKa = 11.84NLEE147 pKa = 4.18GCRR150 pKa = 11.84MEE152 pKa = 6.43DD153 pKa = 3.93YY154 pKa = 10.97IPDD157 pKa = 3.36QSGCFEE163 pKa = 4.37AKK165 pKa = 10.22SIEE168 pKa = 4.21GGTLSVSQDD177 pKa = 3.09FASFPNEE184 pKa = 3.73VRR186 pKa = 11.84LGTAKK191 pKa = 9.86TKK193 pKa = 10.66GKK195 pKa = 9.98IRR197 pKa = 11.84VVTMQGANTKK207 pKa = 9.78KK208 pKa = 9.96VLRR211 pKa = 11.84PVHH214 pKa = 5.9SALYY218 pKa = 10.14DD219 pKa = 3.62YY220 pKa = 11.21LAGFGWLVRR229 pKa = 11.84GDD231 pKa = 3.23VTAADD236 pKa = 4.44FEE238 pKa = 5.4AIIEE242 pKa = 4.31DD243 pKa = 3.65KK244 pKa = 11.22QGDD247 pKa = 3.83EE248 pKa = 4.45KK249 pKa = 11.05FISGDD254 pKa = 3.35YY255 pKa = 9.93EE256 pKa = 3.84QATNHH261 pKa = 5.77INIDD265 pKa = 3.63SVQAIISVIAEE276 pKa = 4.08EE277 pKa = 4.48PLLSDD282 pKa = 3.84EE283 pKa = 4.26EE284 pKa = 4.79RR285 pKa = 11.84EE286 pKa = 3.97VLIRR290 pKa = 11.84SFRR293 pKa = 11.84DD294 pKa = 3.05VTVYY298 pKa = 10.96KK299 pKa = 11.04NNGSFLCKK307 pKa = 10.03VRR309 pKa = 11.84NGSMMGNLVSFPLLCILNKK328 pKa = 10.09CCYY331 pKa = 10.32DD332 pKa = 3.23MSRR335 pKa = 11.84EE336 pKa = 4.0IEE338 pKa = 4.14SEE340 pKa = 4.26EE341 pKa = 4.26NGVPYY346 pKa = 10.2CPRR349 pKa = 11.84VGRR352 pKa = 11.84FNGDD356 pKa = 3.04DD357 pKa = 3.63CAFCGTDD364 pKa = 2.68RR365 pKa = 11.84FFEE368 pKa = 4.06IWRR371 pKa = 11.84EE372 pKa = 3.94TTSIFGLVVQEE383 pKa = 4.29KK384 pKa = 8.57KK385 pKa = 9.37TGISSRR391 pKa = 11.84WIEE394 pKa = 4.03LNSEE398 pKa = 4.16SFDD401 pKa = 3.79SLKK404 pKa = 10.83HH405 pKa = 6.35RR406 pKa = 11.84FVQKK410 pKa = 10.94NFLSYY415 pKa = 11.03LRR417 pKa = 11.84VTRR420 pKa = 11.84DD421 pKa = 3.15TPGDD425 pKa = 3.73LLSEE429 pKa = 4.22IVSGTKK435 pKa = 10.11GFKK438 pKa = 10.61GSTKK442 pKa = 8.54MWLINHH448 pKa = 5.8VLRR451 pKa = 11.84YY452 pKa = 9.07EE453 pKa = 3.72IAIRR457 pKa = 11.84GVCASTIPRR466 pKa = 11.84KK467 pKa = 10.18LFLLLIKK474 pKa = 10.27RR475 pKa = 11.84VWFRR479 pKa = 11.84KK480 pKa = 7.56TLSNPLPPFPTTGVDD495 pKa = 2.98RR496 pKa = 11.84SIRR499 pKa = 11.84QTVVSPPLPSFIPLIDD515 pKa = 5.29DD516 pKa = 3.5IEE518 pKa = 4.4TLVRR522 pKa = 11.84KK523 pKa = 9.48SHH525 pKa = 4.27VKK527 pKa = 9.42KK528 pKa = 9.6WVGVSTIDD536 pKa = 3.87GRR538 pKa = 11.84RR539 pKa = 11.84ARR541 pKa = 11.84CSNEE545 pKa = 3.42MNPDD549 pKa = 3.32HH550 pKa = 7.46DD551 pKa = 3.98HH552 pKa = 6.28FRR554 pKa = 11.84PMVHH558 pKa = 6.65GSLFFPFISSSYY570 pKa = 10.71SPTPLSSSLRR580 pKa = 11.84GSLLRR585 pKa = 11.84QRR587 pKa = 11.84KK588 pKa = 8.19VAFDD592 pKa = 4.49LSSKK596 pKa = 9.99PSASVRR602 pKa = 11.84FSRR605 pKa = 11.84KK606 pKa = 9.11HH607 pKa = 3.98SWGFTMSEE615 pKa = 4.2LCKK618 pKa = 10.35DD619 pKa = 3.33TLTTFFGEE627 pKa = 4.3TWLMPPKK634 pKa = 9.91SLCGSTLPYY643 pKa = 9.57YY644 pKa = 10.46HH645 pKa = 7.38PHH647 pKa = 6.73LKK649 pKa = 9.98IKK651 pKa = 10.52CDD653 pKa = 3.66FLTTVTPTYY662 pKa = 10.04YY663 pKa = 9.97PPPVSLLTTKK673 pKa = 9.91TLAEE677 pKa = 4.2HH678 pKa = 7.0AATIYY683 pKa = 10.58NLEE686 pKa = 4.24RR687 pKa = 11.84TSSSILRR694 pKa = 11.84NSRR697 pKa = 11.84VRR699 pKa = 11.84IAKK702 pKa = 9.81ADD704 pKa = 3.52SKK706 pKa = 10.91LWSFVPDD713 pKa = 3.41NYY715 pKa = 10.1IGSRR719 pKa = 11.84LVVV722 pKa = 3.28

Molecular weight: 81.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S4GA80|A0A0S4GA80_9VIRU RNA dependent RNA polymerase OS=Botrytis ourmia-like virus OX=1872719 GN=RdRp PE=4 SV=1
MM1 pKa = 7.22PTSNDD6 pKa = 2.97MEE8 pKa = 4.97AGCKK12 pKa = 7.73ATRR15 pKa = 11.84SIRR18 pKa = 11.84EE19 pKa = 3.87SLLRR23 pKa = 11.84ATQLISRR30 pKa = 11.84EE31 pKa = 4.01FNLPVPVGPFNGTSCMEE48 pKa = 5.05LRR50 pKa = 11.84SQWDD54 pKa = 3.95TYY56 pKa = 11.65AKK58 pKa = 9.61WSCLQFVSKK67 pKa = 10.7KK68 pKa = 7.33EE69 pKa = 3.96EE70 pKa = 3.8RR71 pKa = 11.84RR72 pKa = 11.84KK73 pKa = 8.43TLVQLIKK80 pKa = 10.56SGCRR84 pKa = 11.84LFDD87 pKa = 4.07GDD89 pKa = 5.98CRR91 pKa = 11.84TCDD94 pKa = 3.43PKK96 pKa = 11.16LKK98 pKa = 10.23EE99 pKa = 3.84LEE101 pKa = 3.83KK102 pKa = 10.76RR103 pKa = 11.84KK104 pKa = 9.19WVEE107 pKa = 3.61QMSTARR113 pKa = 11.84PRR115 pKa = 11.84VAYY118 pKa = 10.27YY119 pKa = 10.52KK120 pKa = 10.87GLTRR124 pKa = 11.84STWLGRR130 pKa = 11.84LKK132 pKa = 10.9DD133 pKa = 3.65SARR136 pKa = 11.84EE137 pKa = 4.01LITGWGRR144 pKa = 11.84NLEE147 pKa = 4.18GCRR150 pKa = 11.84MEE152 pKa = 6.43DD153 pKa = 3.93YY154 pKa = 10.97IPDD157 pKa = 3.36QSGCFEE163 pKa = 4.37AKK165 pKa = 10.22SIEE168 pKa = 4.21GGTLSVSQDD177 pKa = 3.09FASFPNEE184 pKa = 3.73VRR186 pKa = 11.84LGTAKK191 pKa = 9.86TKK193 pKa = 10.66GKK195 pKa = 9.98IRR197 pKa = 11.84VVTMQGANTKK207 pKa = 9.78KK208 pKa = 9.96VLRR211 pKa = 11.84PVHH214 pKa = 5.9SALYY218 pKa = 10.14DD219 pKa = 3.62YY220 pKa = 11.21LAGFGWLVRR229 pKa = 11.84GDD231 pKa = 3.23VTAADD236 pKa = 4.44FEE238 pKa = 5.4AIIEE242 pKa = 4.31DD243 pKa = 3.65KK244 pKa = 11.22QGDD247 pKa = 3.83EE248 pKa = 4.45KK249 pKa = 11.05FISGDD254 pKa = 3.35YY255 pKa = 9.93EE256 pKa = 3.84QATNHH261 pKa = 5.77INIDD265 pKa = 3.63SVQAIISVIAEE276 pKa = 4.08EE277 pKa = 4.48PLLSDD282 pKa = 3.84EE283 pKa = 4.26EE284 pKa = 4.79RR285 pKa = 11.84EE286 pKa = 3.97VLIRR290 pKa = 11.84SFRR293 pKa = 11.84DD294 pKa = 3.05VTVYY298 pKa = 10.96KK299 pKa = 11.04NNGSFLCKK307 pKa = 10.03VRR309 pKa = 11.84NGSMMGNLVSFPLLCILNKK328 pKa = 10.09CCYY331 pKa = 10.32DD332 pKa = 3.23MSRR335 pKa = 11.84EE336 pKa = 4.0IEE338 pKa = 4.14SEE340 pKa = 4.26EE341 pKa = 4.26NGVPYY346 pKa = 10.2CPRR349 pKa = 11.84VGRR352 pKa = 11.84FNGDD356 pKa = 3.04DD357 pKa = 3.63CAFCGTDD364 pKa = 2.68RR365 pKa = 11.84FFEE368 pKa = 4.06IWRR371 pKa = 11.84EE372 pKa = 3.94TTSIFGLVVQEE383 pKa = 4.29KK384 pKa = 8.57KK385 pKa = 9.37TGISSRR391 pKa = 11.84WIEE394 pKa = 4.03LNSEE398 pKa = 4.16SFDD401 pKa = 3.79SLKK404 pKa = 10.83HH405 pKa = 6.35RR406 pKa = 11.84FVQKK410 pKa = 10.94NFLSYY415 pKa = 11.03LRR417 pKa = 11.84VTRR420 pKa = 11.84DD421 pKa = 3.15TPGDD425 pKa = 3.73LLSEE429 pKa = 4.22IVSGTKK435 pKa = 10.11GFKK438 pKa = 10.61GSTKK442 pKa = 8.54MWLINHH448 pKa = 5.8VLRR451 pKa = 11.84YY452 pKa = 9.07EE453 pKa = 3.72IAIRR457 pKa = 11.84GVCASTIPRR466 pKa = 11.84KK467 pKa = 10.18LFLLLIKK474 pKa = 10.27RR475 pKa = 11.84VWFRR479 pKa = 11.84KK480 pKa = 7.56TLSNPLPPFPTTGVDD495 pKa = 2.98RR496 pKa = 11.84SIRR499 pKa = 11.84QTVVSPPLPSFIPLIDD515 pKa = 5.29DD516 pKa = 3.5IEE518 pKa = 4.4TLVRR522 pKa = 11.84KK523 pKa = 9.48SHH525 pKa = 4.27VKK527 pKa = 9.42KK528 pKa = 9.6WVGVSTIDD536 pKa = 3.87GRR538 pKa = 11.84RR539 pKa = 11.84ARR541 pKa = 11.84CSNEE545 pKa = 3.42MNPDD549 pKa = 3.32HH550 pKa = 7.46DD551 pKa = 3.98HH552 pKa = 6.28FRR554 pKa = 11.84PMVHH558 pKa = 6.65GSLFFPFISSSYY570 pKa = 10.71SPTPLSSSLRR580 pKa = 11.84GSLLRR585 pKa = 11.84QRR587 pKa = 11.84KK588 pKa = 8.19VAFDD592 pKa = 4.49LSSKK596 pKa = 9.99PSASVRR602 pKa = 11.84FSRR605 pKa = 11.84KK606 pKa = 9.11HH607 pKa = 3.98SWGFTMSEE615 pKa = 4.2LCKK618 pKa = 10.35DD619 pKa = 3.33TLTTFFGEE627 pKa = 4.3TWLMPPKK634 pKa = 9.91SLCGSTLPYY643 pKa = 9.57YY644 pKa = 10.46HH645 pKa = 7.38PHH647 pKa = 6.73LKK649 pKa = 9.98IKK651 pKa = 10.52CDD653 pKa = 3.66FLTTVTPTYY662 pKa = 10.04YY663 pKa = 9.97PPPVSLLTTKK673 pKa = 9.91TLAEE677 pKa = 4.2HH678 pKa = 7.0AATIYY683 pKa = 10.58NLEE686 pKa = 4.24RR687 pKa = 11.84TSSSILRR694 pKa = 11.84NSRR697 pKa = 11.84VRR699 pKa = 11.84IAKK702 pKa = 9.81ADD704 pKa = 3.52SKK706 pKa = 10.91LWSFVPDD713 pKa = 3.41NYY715 pKa = 10.1IGSRR719 pKa = 11.84LVVV722 pKa = 3.28

Molecular weight: 81.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

722

722

722

722.0

81.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.155 ± 0.0

2.77 ± 0.0

4.986 ± 0.0

5.679 ± 0.0

5.125 ± 0.0

6.094 ± 0.0

1.662 ± 0.0

5.402 ± 0.0

6.371 ± 0.0

9.418 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.939 ± 0.0

3.324 ± 0.0

5.263 ± 0.0

2.078 ± 0.0

7.756 ± 0.0

9.972 ± 0.0

6.925 ± 0.0

6.51 ± 0.0

1.939 ± 0.0

2.632 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski