Pantoea phage vB_PagS_Vid5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vidquintavirus; Pantoea virus Vid5

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1CKR2|A0A2P1CKR2_9CAUD GTP cyclohydrolase I OS=Pantoea phage vB_PagS_Vid5 OX=2099652 GN=Vid5_gp35 PE=3 SV=1
MM1 pKa = 7.58TGPSNALGLYY11 pKa = 9.67IVGALCGDD19 pKa = 3.57TRR21 pKa = 11.84LKK23 pKa = 10.56KK24 pKa = 9.93VSNMEE29 pKa = 4.28TLLSLPGNTFRR40 pKa = 11.84GIYY43 pKa = 9.37DD44 pKa = 3.3EE45 pKa = 5.66CDD47 pKa = 3.05RR48 pKa = 11.84CDD50 pKa = 3.28WTGVLYY56 pKa = 11.09NGVYY60 pKa = 9.31YY61 pKa = 10.77SEE63 pKa = 4.76GEE65 pKa = 4.32VYY67 pKa = 10.06FASLQRR73 pKa = 11.84DD74 pKa = 3.35EE75 pKa = 5.32GYY77 pKa = 10.85EE78 pKa = 3.75ILLL81 pKa = 4.16

Molecular weight: 9.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1CKM5|A0A2P1CKM5_9CAUD DNA binding protein OS=Pantoea phage vB_PagS_Vid5 OX=2099652 GN=Vid5_gp52 PE=4 SV=1
MM1 pKa = 7.71PSSSKK6 pKa = 9.14NTSGPLLNTASRR18 pKa = 11.84LRR20 pKa = 11.84IVAFTLNSYY29 pKa = 7.24WRR31 pKa = 11.84RR32 pKa = 11.84CKK34 pKa = 9.85PASSLSSSRR43 pKa = 11.84NCYY46 pKa = 9.74HH47 pKa = 7.48LYY49 pKa = 10.78LHH51 pKa = 6.96LPDD54 pKa = 5.16KK55 pKa = 11.23NFLKK59 pKa = 10.89

Molecular weight: 6.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

99

0

99

19673

34

1087

198.7

22.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.368 ± 0.567

1.454 ± 0.168

5.378 ± 0.152

5.81 ± 0.245

3.701 ± 0.221

7.315 ± 0.223

1.942 ± 0.172

5.302 ± 0.186

5.5 ± 0.257

8.001 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.852 ± 0.145

4.783 ± 0.169

4.519 ± 0.299

4.356 ± 0.329

5.083 ± 0.149

6.13 ± 0.275

5.952 ± 0.219

7.467 ± 0.149

1.525 ± 0.114

3.563 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski