Cacao swollen shoot Ghana R virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Caulimoviridae; Badnavirus; unclassified Badnavirus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2H4U983|A0A2H4U983_9VIRU ORF2 protein OS=Cacao swollen shoot Ghana R virus OX=2056886 PE=4 SV=1
MM1 pKa = 7.1FRR3 pKa = 11.84LGEE6 pKa = 4.14GMAGSSKK13 pKa = 8.2QTIGEE18 pKa = 4.28KK19 pKa = 10.21FRR21 pKa = 11.84SPGGNQFNLAKK32 pKa = 9.9EE33 pKa = 4.17YY34 pKa = 11.04KK35 pKa = 9.91DD36 pKa = 3.77LCDD39 pKa = 3.63DD40 pKa = 3.53EE41 pKa = 5.95RR42 pKa = 11.84RR43 pKa = 11.84ITAEE47 pKa = 4.62GIPLPLLQAYY57 pKa = 6.95QHH59 pKa = 5.93EE60 pKa = 4.37RR61 pKa = 11.84ALCEE65 pKa = 4.0EE66 pKa = 4.23KK67 pKa = 11.09AEE69 pKa = 4.07NLLLEE74 pKa = 4.54AMEE77 pKa = 3.83EE78 pKa = 4.1LIRR81 pKa = 11.84IKK83 pKa = 9.74EE84 pKa = 4.02AKK86 pKa = 8.34RR87 pKa = 11.84AYY89 pKa = 9.36VASHH93 pKa = 5.78ATKK96 pKa = 10.57DD97 pKa = 3.91NYY99 pKa = 10.33WGDD102 pKa = 3.93CLPTVKK108 pKa = 10.48DD109 pKa = 3.53AEE111 pKa = 4.37DD112 pKa = 3.5RR113 pKa = 11.84TKK115 pKa = 10.89DD116 pKa = 3.14VCNALRR122 pKa = 11.84QVVEE126 pKa = 4.04NSKK129 pKa = 10.9DD130 pKa = 3.18RR131 pKa = 11.84YY132 pKa = 9.92

Molecular weight:
15.05 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2H4U988|A0A2H4U988_9VIRU ORF1 protein OS=Cacao swollen shoot Ghana R virus OX=2056886 PE=4 SV=1
MM1 pKa = 7.22GWLMSGNSYY10 pKa = 10.81QEE12 pKa = 3.93AFRR15 pKa = 11.84NAAAYY20 pKa = 9.37KK21 pKa = 8.49PTGFLKK27 pKa = 10.7SEE29 pKa = 3.99EE30 pKa = 4.25AGSGRR35 pKa = 11.84EE36 pKa = 3.87QLLSCISQHH45 pKa = 6.14NLIIQLLVDD54 pKa = 3.95LHH56 pKa = 7.13DD57 pKa = 4.52KK58 pKa = 10.79VDD60 pKa = 3.71NLSAEE65 pKa = 4.31LKK67 pKa = 10.27VLRR70 pKa = 11.84KK71 pKa = 9.73EE72 pKa = 3.84KK73 pKa = 10.25TKK75 pKa = 10.76AQNPEE80 pKa = 4.23EE81 pKa = 4.19IEE83 pKa = 4.13GLISQLKK90 pKa = 9.3GLSIGPKK97 pKa = 9.41EE98 pKa = 4.07KK99 pKa = 10.92VPEE102 pKa = 4.2KK103 pKa = 10.58KK104 pKa = 10.65GNLYY108 pKa = 10.63AFKK111 pKa = 10.35DD112 pKa = 3.7PYY114 pKa = 11.12VILEE118 pKa = 4.03EE119 pKa = 4.33VKK121 pKa = 10.75SKK123 pKa = 10.85KK124 pKa = 10.31

Molecular weight:
13.89 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2227

124

1833

556.8

63.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.658 ± 0.905

1.706 ± 0.426

5.209 ± 0.812

8.487 ± 1.624

3.233 ± 0.486

5.972 ± 0.98

2.066 ± 0.179

7.185 ± 0.948

7.05 ± 1.766

7.634 ± 2.638

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.425 ± 0.481

4.535 ± 1.041

4.535 ± 0.9

6.286 ± 0.673

6.421 ± 0.98

6.691 ± 0.584

5.029 ± 1.004

4.894 ± 0.437

1.482 ± 0.371

3.502 ± 0.43

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski