Escherichia phage vB_EcoM_WFbE185

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Mosigvirus; unclassified Mosigvirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 277 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482MWM7|A0A482MWM7_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoM_WFbE185 OX=2508199 GN=WFbE185_00127 PE=4 SV=1
MM1 pKa = 7.74AKK3 pKa = 10.39LIIEE7 pKa = 4.36GSEE10 pKa = 4.04DD11 pKa = 3.47VLKK14 pKa = 11.17CFAAWFSCSGEE25 pKa = 3.75QSFIEE30 pKa = 4.39SFRR33 pKa = 11.84MGDD36 pKa = 2.78ITGNYY41 pKa = 7.15PATDD45 pKa = 3.09ITVRR49 pKa = 11.84GYY51 pKa = 11.13GINEE55 pKa = 4.97PIQLVEE61 pKa = 4.14YY62 pKa = 10.82DD63 pKa = 3.76LATDD67 pKa = 3.6EE68 pKa = 5.02EE69 pKa = 4.67IPYY72 pKa = 10.43VDD74 pKa = 3.51

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482MV55|A0A482MV55_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoM_WFbE185 OX=2508199 GN=WFbE185_00093 PE=4 SV=1
MM1 pKa = 6.94KK2 pKa = 10.19TEE4 pKa = 4.41LFYY7 pKa = 11.55GEE9 pKa = 4.18RR10 pKa = 11.84TNGQKK15 pKa = 9.2SWKK18 pKa = 9.35FIMIGAPNNPNMRR31 pKa = 11.84TSIISVTRR39 pKa = 11.84PTKK42 pKa = 10.68RR43 pKa = 11.84MIRR46 pKa = 11.84QYY48 pKa = 11.46KK49 pKa = 8.81RR50 pKa = 11.84FHH52 pKa = 6.58RR53 pKa = 11.84DD54 pKa = 2.98FYY56 pKa = 11.72NVYY59 pKa = 10.65

Molecular weight: 7.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

277

0

277

53796

36

1291

194.2

22.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.699 ± 0.159

1.073 ± 0.066

6.309 ± 0.116

7.274 ± 0.163

4.389 ± 0.106

6.127 ± 0.192

1.716 ± 0.079

7.201 ± 0.136

7.577 ± 0.221

7.495 ± 0.129

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.617 ± 0.091

5.539 ± 0.14

3.526 ± 0.104

3.318 ± 0.096

4.333 ± 0.096

6.348 ± 0.129

6.049 ± 0.241

6.727 ± 0.118

1.387 ± 0.051

4.296 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski