Bacteroides helcogenes (strain ATCC 35417 / DSM 20613 / JCM 6297 / P 36-108)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides helcogenes

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3240 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E6STG3|E6STG3_BACT6 Uncharacterized protein OS=Bacteroides helcogenes (strain ATCC 35417 / DSM 20613 / JCM 6297 / P 36-108) OX=693979 GN=Bache_1227 PE=4 SV=1
MM1 pKa = 7.32GLEE4 pKa = 5.45DD5 pKa = 6.18DD6 pKa = 5.35FLQNDD11 pKa = 3.23VDD13 pKa = 4.03DD14 pKa = 5.07EE15 pKa = 4.35KK16 pKa = 10.68TIEE19 pKa = 4.15YY20 pKa = 9.37IKK22 pKa = 10.96NYY24 pKa = 9.95LPQEE28 pKa = 4.16LKK30 pKa = 10.91EE31 pKa = 4.3KK32 pKa = 10.78FSDD35 pKa = 3.62DD36 pKa = 3.07EE37 pKa = 4.59FYY39 pKa = 11.59YY40 pKa = 10.87FLDD43 pKa = 5.51LIDD46 pKa = 4.21EE47 pKa = 5.42FYY49 pKa = 10.6SQSGILDD56 pKa = 3.79AQPDD60 pKa = 3.73EE61 pKa = 4.98DD62 pKa = 5.37GYY64 pKa = 11.03IEE66 pKa = 4.78IDD68 pKa = 3.06LGKK71 pKa = 10.2IVDD74 pKa = 4.27YY75 pKa = 10.53IVKK78 pKa = 9.51EE79 pKa = 3.86AHH81 pKa = 6.38KK82 pKa = 10.85DD83 pKa = 3.52EE84 pKa = 4.7MGDD87 pKa = 3.61YY88 pKa = 10.68DD89 pKa = 3.9PEE91 pKa = 5.28EE92 pKa = 3.8ILFIVQGEE100 pKa = 4.27MEE102 pKa = 4.31YY103 pKa = 10.49TEE105 pKa = 4.39SLEE108 pKa = 4.17EE109 pKa = 3.96

Molecular weight: 12.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E6SNW4|E6SNW4_BACT6 Uroporphyrinogen III synthase HEM4 OS=Bacteroides helcogenes (strain ATCC 35417 / DSM 20613 / JCM 6297 / P 36-108) OX=693979 GN=Bache_0760 PE=4 SV=1
MM1 pKa = 7.7KK2 pKa = 9.95IYY4 pKa = 10.65AEE6 pKa = 4.32KK7 pKa = 10.82GMSRR11 pKa = 11.84DD12 pKa = 3.27LQGRR16 pKa = 11.84KK17 pKa = 8.88KK18 pKa = 10.51AFRR21 pKa = 11.84IALRR25 pKa = 11.84IGNRR29 pKa = 11.84RR30 pKa = 11.84PAACVLAFFSVVASYY45 pKa = 11.84SNLSTCCFRR54 pKa = 11.84AQSS57 pKa = 3.43

Molecular weight: 6.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3240

0

3240

1182685

30

1954

365.0

41.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.314 ± 0.044

1.328 ± 0.016

5.447 ± 0.03

6.384 ± 0.045

4.549 ± 0.03

6.952 ± 0.032

1.92 ± 0.017

6.81 ± 0.038

6.484 ± 0.036

9.236 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.817 ± 0.019

5.048 ± 0.042

3.677 ± 0.025

3.337 ± 0.022

4.774 ± 0.029

6.15 ± 0.035

5.658 ± 0.035

6.421 ± 0.033

1.23 ± 0.017

4.465 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski