Amino acid dipepetide frequency for Novosphingobium sp. Fuku2-ISO-50

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.11AlaAla: 20.11 ± 0.215
1.164AlaCys: 1.164 ± 0.033
7.944AlaAsp: 7.944 ± 0.092
7.21AlaGlu: 7.21 ± 0.096
4.44AlaPhe: 4.44 ± 0.059
12.152AlaGly: 12.152 ± 0.113
3.111AlaHis: 3.111 ± 0.053
7.025AlaIle: 7.025 ± 0.079
3.524AlaLys: 3.524 ± 0.066
15.402AlaLeu: 15.402 ± 0.149
4.263AlaMet: 4.263 ± 0.066
3.219AlaAsn: 3.219 ± 0.057
7.004AlaPro: 7.004 ± 0.099
4.943AlaGln: 4.943 ± 0.067
10.994AlaArg: 10.994 ± 0.117
6.4AlaSer: 6.4 ± 0.076
6.84AlaThr: 6.84 ± 0.093
8.965AlaVal: 8.965 ± 0.1
1.875AlaTrp: 1.875 ± 0.04
2.719AlaTyr: 2.719 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
1.127CysAla: 1.127 ± 0.034
0.109CysCys: 0.109 ± 0.009
0.574CysAsp: 0.574 ± 0.019
0.413CysGlu: 0.413 ± 0.018
0.306CysPhe: 0.306 ± 0.016
0.959CysGly: 0.959 ± 0.031
0.256CysHis: 0.256 ± 0.014
0.347CysIle: 0.347 ± 0.017
0.16CysLys: 0.16 ± 0.012
0.787CysLeu: 0.787 ± 0.027
0.155CysMet: 0.155 ± 0.011
0.225CysAsn: 0.225 ± 0.013
0.518CysPro: 0.518 ± 0.022
0.226CysGln: 0.226 ± 0.015
0.575CysArg: 0.575 ± 0.023
0.468CysSer: 0.468 ± 0.02
0.424CysThr: 0.424 ± 0.02
0.587CysVal: 0.587 ± 0.022
0.123CysTrp: 0.123 ± 0.01
0.192CysTyr: 0.192 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
7.852AspAla: 7.852 ± 0.092
0.54AspCys: 0.54 ± 0.02
3.339AspAsp: 3.339 ± 0.059
3.117AspGlu: 3.117 ± 0.058
2.11AspPhe: 2.11 ± 0.037
5.338AspGly: 5.338 ± 0.068
1.631AspHis: 1.631 ± 0.035
2.705AspIle: 2.705 ± 0.045
1.577AspLys: 1.577 ± 0.037
6.048AspLeu: 6.048 ± 0.071
1.356AspMet: 1.356 ± 0.032
1.33AspAsn: 1.33 ± 0.032
3.966AspPro: 3.966 ± 0.064
1.771AspGln: 1.771 ± 0.034
4.683AspArg: 4.683 ± 0.061
2.306AspSer: 2.306 ± 0.045
2.785AspThr: 2.785 ± 0.049
4.205AspVal: 4.205 ± 0.05
1.207AspTrp: 1.207 ± 0.034
1.558AspTyr: 1.558 ± 0.04
0.0AspXaa: 0.0 ± 0.0
Glu
7.556GluAla: 7.556 ± 0.099
0.368GluCys: 0.368 ± 0.015
2.43GluAsp: 2.43 ± 0.054
2.236GluGlu: 2.236 ± 0.056
1.5GluPhe: 1.5 ± 0.035
3.913GluGly: 3.913 ± 0.065
1.114GluHis: 1.114 ± 0.03
2.656GluIle: 2.656 ± 0.048
1.48GluLys: 1.48 ± 0.037
4.447GluLeu: 4.447 ± 0.053
1.187GluMet: 1.187 ± 0.03
1.181GluAsn: 1.181 ± 0.035
2.429GluPro: 2.429 ± 0.048
1.784GluGln: 1.784 ± 0.041
4.299GluArg: 4.299 ± 0.067
2.08GluSer: 2.08 ± 0.04
3.02GluThr: 3.02 ± 0.051
3.315GluVal: 3.315 ± 0.057
0.773GluTrp: 0.773 ± 0.027
0.891GluTyr: 0.891 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
5.272PheAla: 5.272 ± 0.06
0.359PheCys: 0.359 ± 0.017
2.776PheAsp: 2.776 ± 0.045
1.734PheGlu: 1.734 ± 0.039
1.2PhePhe: 1.2 ± 0.03
3.809PheGly: 3.809 ± 0.06
0.81PheHis: 0.81 ± 0.025
1.385PheIle: 1.385 ± 0.034
0.76PheLys: 0.76 ± 0.027
2.906PheLeu: 2.906 ± 0.056
0.759PheMet: 0.759 ± 0.027
1.022PheAsn: 1.022 ± 0.03
1.545PhePro: 1.545 ± 0.034
0.832PheGln: 0.832 ± 0.029
2.123PheArg: 2.123 ± 0.043
1.924PheSer: 1.924 ± 0.042
1.97PheThr: 1.97 ± 0.04
2.675PheVal: 2.675 ± 0.05
0.466PheTrp: 0.466 ± 0.022
0.952PheTyr: 0.952 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
11.005GlyAla: 11.005 ± 0.118
0.851GlyCys: 0.851 ± 0.024
4.93GlyAsp: 4.93 ± 0.067
4.618GlyGlu: 4.618 ± 0.067
3.687GlyPhe: 3.687 ± 0.056
8.301GlyGly: 8.301 ± 0.125
2.31GlyHis: 2.31 ± 0.046
4.448GlyIle: 4.448 ± 0.062
3.049GlyLys: 3.049 ± 0.053
9.152GlyLeu: 9.152 ± 0.094
2.379GlyMet: 2.379 ± 0.047
2.308GlyAsn: 2.308 ± 0.054
3.989GlyPro: 3.989 ± 0.06
3.079GlyGln: 3.079 ± 0.054
6.21GlyArg: 6.21 ± 0.076
4.767GlySer: 4.767 ± 0.074
5.192GlyThr: 5.192 ± 0.091
6.472GlyVal: 6.472 ± 0.078
1.666GlyTrp: 1.666 ± 0.039
2.493GlyTyr: 2.493 ± 0.055
0.0GlyXaa: 0.0 ± 0.0
His
3.038HisAla: 3.038 ± 0.055
0.278HisCys: 0.278 ± 0.015
1.592HisAsp: 1.592 ± 0.04
1.083HisGlu: 1.083 ± 0.028
0.972HisPhe: 0.972 ± 0.028
2.401HisGly: 2.401 ± 0.041
0.681HisHis: 0.681 ± 0.028
0.976HisIle: 0.976 ± 0.027
0.485HisLys: 0.485 ± 0.025
2.173HisLeu: 2.173 ± 0.044
0.47HisMet: 0.47 ± 0.02
0.478HisAsn: 0.478 ± 0.019
1.518HisPro: 1.518 ± 0.037
0.61HisGln: 0.61 ± 0.023
1.625HisArg: 1.625 ± 0.039
0.978HisSer: 0.978 ± 0.031
0.904HisThr: 0.904 ± 0.025
1.809HisVal: 1.809 ± 0.034
0.399HisTrp: 0.399 ± 0.016
0.675HisTyr: 0.675 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
8.517IleAla: 8.517 ± 0.102
0.473IleCys: 0.473 ± 0.019
4.03IleAsp: 4.03 ± 0.056
3.282IleGlu: 3.282 ± 0.051
1.282IlePhe: 1.282 ± 0.036
5.125IleGly: 5.125 ± 0.074
0.978IleHis: 0.978 ± 0.029
1.8IleIle: 1.8 ± 0.041
1.188IleLys: 1.188 ± 0.033
3.548IleLeu: 3.548 ± 0.054
0.969IleMet: 0.969 ± 0.03
1.48IleAsn: 1.48 ± 0.038
2.318IlePro: 2.318 ± 0.048
1.083IleGln: 1.083 ± 0.034
3.014IleArg: 3.014 ± 0.043
2.516IleSer: 2.516 ± 0.047
2.695IleThr: 2.695 ± 0.055
3.816IleVal: 3.816 ± 0.059
0.574IleTrp: 0.574 ± 0.019
1.062IleTyr: 1.062 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
3.713LysAla: 3.713 ± 0.066
0.133LysCys: 0.133 ± 0.011
1.334LysAsp: 1.334 ± 0.04
0.907LysGlu: 0.907 ± 0.031
0.766LysPhe: 0.766 ± 0.025
2.372LysGly: 2.372 ± 0.052
0.45LysHis: 0.45 ± 0.02
1.216LysIle: 1.216 ± 0.03
0.828LysLys: 0.828 ± 0.031
2.716LysLeu: 2.716 ± 0.056
0.559LysMet: 0.559 ± 0.022
0.592LysAsn: 0.592 ± 0.021
1.696LysPro: 1.696 ± 0.046
0.781LysGln: 0.781 ± 0.027
1.744LysArg: 1.744 ± 0.044
1.386LysSer: 1.386 ± 0.037
1.528LysThr: 1.528 ± 0.038
2.114LysVal: 2.114 ± 0.045
0.365LysTrp: 0.365 ± 0.02
0.54LysTyr: 0.54 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
15.545LeuAla: 15.545 ± 0.162
0.839LeuCys: 0.839 ± 0.025
6.33LeuAsp: 6.33 ± 0.073
4.195LeuGlu: 4.195 ± 0.068
3.481LeuPhe: 3.481 ± 0.06
8.959LeuGly: 8.959 ± 0.099
2.091LeuHis: 2.091 ± 0.045
5.152LeuIle: 5.152 ± 0.066
2.612LeuLys: 2.612 ± 0.053
8.617LeuLeu: 8.617 ± 0.101
2.024LeuMet: 2.024 ± 0.04
2.241LeuAsn: 2.241 ± 0.046
5.717LeuPro: 5.717 ± 0.071
2.394LeuGln: 2.394 ± 0.042
6.777LeuArg: 6.777 ± 0.072
5.794LeuSer: 5.794 ± 0.071
5.692LeuThr: 5.692 ± 0.07
7.573LeuVal: 7.573 ± 0.095
1.198LeuTrp: 1.198 ± 0.03
1.88LeuTyr: 1.88 ± 0.042
0.0LeuXaa: 0.0 ± 0.0
Met
3.604MetAla: 3.604 ± 0.058
0.157MetCys: 0.157 ± 0.013
1.034MetAsp: 1.034 ± 0.029
0.893MetGlu: 0.893 ± 0.026
0.65MetPhe: 0.65 ± 0.026
1.907MetGly: 1.907 ± 0.04
0.491MetHis: 0.491 ± 0.017
1.407MetIle: 1.407 ± 0.041
0.741MetLys: 0.741 ± 0.019
2.519MetLeu: 2.519 ± 0.052
0.646MetMet: 0.646 ± 0.026
0.629MetAsn: 0.629 ± 0.021
1.497MetPro: 1.497 ± 0.036
0.741MetGln: 0.741 ± 0.024
1.783MetArg: 1.783 ± 0.038
1.299MetSer: 1.299 ± 0.035
1.843MetThr: 1.843 ± 0.031
1.806MetVal: 1.806 ± 0.044
0.189MetTrp: 0.189 ± 0.011
0.238MetTyr: 0.238 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.428AsnAla: 3.428 ± 0.059
0.252AsnCys: 0.252 ± 0.015
1.313AsnAsp: 1.313 ± 0.033
0.973AsnGlu: 0.973 ± 0.029
0.924AsnPhe: 0.924 ± 0.028
2.424AsnGly: 2.424 ± 0.062
0.515AsnHis: 0.515 ± 0.021
1.112AsnIle: 1.112 ± 0.034
0.526AsnLys: 0.526 ± 0.024
2.401AsnLeu: 2.401 ± 0.045
0.539AsnMet: 0.539 ± 0.02
0.684AsnAsn: 0.684 ± 0.025
1.908AsnPro: 1.908 ± 0.04
0.732AsnGln: 0.732 ± 0.026
1.771AsnArg: 1.771 ± 0.036
1.211AsnSer: 1.211 ± 0.043
1.315AsnThr: 1.315 ± 0.037
1.864AsnVal: 1.864 ± 0.047
0.421AsnTrp: 0.421 ± 0.02
0.678AsnTyr: 0.678 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
7.54ProAla: 7.54 ± 0.098
0.36ProCys: 0.36 ± 0.018
4.059ProAsp: 4.059 ± 0.056
3.269ProGlu: 3.269 ± 0.054
1.922ProPhe: 1.922 ± 0.04
5.414ProGly: 5.414 ± 0.075
1.25ProHis: 1.25 ± 0.036
2.559ProIle: 2.559 ± 0.044
1.288ProLys: 1.288 ± 0.041
5.13ProLeu: 5.13 ± 0.06
1.222ProMet: 1.222 ± 0.033
1.175ProAsn: 1.175 ± 0.031
2.87ProPro: 2.87 ± 0.071
1.747ProGln: 1.747 ± 0.036
3.275ProArg: 3.275 ± 0.058
2.517ProSer: 2.517 ± 0.051
2.655ProThr: 2.655 ± 0.045
4.608ProVal: 4.608 ± 0.065
0.802ProTrp: 0.802 ± 0.026
1.08ProTyr: 1.08 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
4.335GlnAla: 4.335 ± 0.062
0.243GlnCys: 0.243 ± 0.014
1.371GlnAsp: 1.371 ± 0.032
1.09GlnGlu: 1.09 ± 0.033
1.162GlnPhe: 1.162 ± 0.028
2.67GlnGly: 2.67 ± 0.044
0.645GlnHis: 0.645 ± 0.022
1.807GlnIle: 1.807 ± 0.04
0.801GlnLys: 0.801 ± 0.026
2.7GlnLeu: 2.7 ± 0.045
0.827GlnMet: 0.827 ± 0.026
0.76GlnAsn: 0.76 ± 0.028
1.812GlnPro: 1.812 ± 0.04
1.066GlnGln: 1.066 ± 0.031
2.272GlnArg: 2.272 ± 0.042
1.739GlnSer: 1.739 ± 0.041
1.828GlnThr: 1.828 ± 0.044
2.388GlnVal: 2.388 ± 0.043
0.531GlnTrp: 0.531 ± 0.02
0.652GlnTyr: 0.652 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
9.558ArgAla: 9.558 ± 0.114
0.505ArgCys: 0.505 ± 0.021
4.231ArgAsp: 4.231 ± 0.058
3.733ArgGlu: 3.733 ± 0.058
3.102ArgPhe: 3.102 ± 0.056
4.974ArgGly: 4.974 ± 0.062
1.967ArgHis: 1.967 ± 0.041
4.032ArgIle: 4.032 ± 0.068
1.894ArgLys: 1.894 ± 0.04
8.413ArgLeu: 8.413 ± 0.098
1.823ArgMet: 1.823 ± 0.043
1.734ArgAsn: 1.734 ± 0.041
3.63ArgPro: 3.63 ± 0.049
2.372ArgGln: 2.372 ± 0.04
5.563ArgArg: 5.563 ± 0.081
3.206ArgSer: 3.206 ± 0.044
3.249ArgThr: 3.249 ± 0.051
4.823ArgVal: 4.823 ± 0.06
1.168ArgTrp: 1.168 ± 0.033
1.769ArgTyr: 1.769 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
6.501SerAla: 6.501 ± 0.085
0.402SerCys: 0.402 ± 0.017
2.851SerAsp: 2.851 ± 0.055
2.28SerGlu: 2.28 ± 0.047
1.988SerPhe: 1.988 ± 0.04
5.529SerGly: 5.529 ± 0.097
1.141SerHis: 1.141 ± 0.03
2.382SerIle: 2.382 ± 0.044
1.12SerLys: 1.12 ± 0.032
5.069SerLeu: 5.069 ± 0.079
1.063SerMet: 1.063 ± 0.03
1.341SerAsn: 1.341 ± 0.039
2.828SerPro: 2.828 ± 0.047
1.529SerGln: 1.529 ± 0.037
3.15SerArg: 3.15 ± 0.051
2.655SerSer: 2.655 ± 0.059
2.574SerThr: 2.574 ± 0.056
3.565SerVal: 3.565 ± 0.056
0.727SerTrp: 0.727 ± 0.023
1.306SerTyr: 1.306 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
6.498ThrAla: 6.498 ± 0.093
0.46ThrCys: 0.46 ± 0.02
2.79ThrAsp: 2.79 ± 0.049
2.13ThrGlu: 2.13 ± 0.044
1.888ThrPhe: 1.888 ± 0.04
5.819ThrGly: 5.819 ± 0.087
1.13ThrHis: 1.13 ± 0.031
3.078ThrIle: 3.078 ± 0.055
1.074ThrLys: 1.074 ± 0.034
5.885ThrLeu: 5.885 ± 0.075
1.305ThrMet: 1.305 ± 0.033
1.459ThrAsn: 1.459 ± 0.044
3.381ThrPro: 3.381 ± 0.057
1.591ThrGln: 1.591 ± 0.037
3.565ThrArg: 3.565 ± 0.056
2.766ThrSer: 2.766 ± 0.057
3.018ThrThr: 3.018 ± 0.07
4.152ThrVal: 4.152 ± 0.069
0.712ThrTrp: 0.712 ± 0.023
1.308ThrTyr: 1.308 ± 0.035
0.0ThrXaa: 0.0 ± 0.0
Val
9.801ValAla: 9.801 ± 0.099
0.624ValCys: 0.624 ± 0.023
4.191ValAsp: 4.191 ± 0.056
3.93ValGlu: 3.93 ± 0.06
2.468ValPhe: 2.468 ± 0.045
5.61ValGly: 5.61 ± 0.077
1.539ValHis: 1.539 ± 0.036
4.141ValIle: 4.141 ± 0.059
1.696ValLys: 1.696 ± 0.043
7.291ValLeu: 7.291 ± 0.091
1.77ValMet: 1.77 ± 0.038
1.978ValAsn: 1.978 ± 0.046
4.236ValPro: 4.236 ± 0.068
1.912ValGln: 1.912 ± 0.038
5.123ValArg: 5.123 ± 0.062
3.927ValSer: 3.927 ± 0.058
4.465ValThr: 4.465 ± 0.07
5.601ValVal: 5.601 ± 0.093
0.995ValTrp: 0.995 ± 0.03
1.429ValTyr: 1.429 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.452TrpAla: 1.452 ± 0.034
0.156TrpCys: 0.156 ± 0.011
0.764TrpAsp: 0.764 ± 0.024
0.544TrpGlu: 0.544 ± 0.019
0.609TrpPhe: 0.609 ± 0.022
1.044TrpGly: 1.044 ± 0.025
0.455TrpHis: 0.455 ± 0.02
0.698TrpIle: 0.698 ± 0.022
0.403TrpLys: 0.403 ± 0.019
1.854TrpLeu: 1.854 ± 0.048
0.358TrpMet: 0.358 ± 0.016
0.434TrpAsn: 0.434 ± 0.016
0.786TrpPro: 0.786 ± 0.025
0.733TrpGln: 0.733 ± 0.025
1.369TrpArg: 1.369 ± 0.037
0.908TrpSer: 0.908 ± 0.028
0.809TrpThr: 0.809 ± 0.024
0.86TrpVal: 0.86 ± 0.025
0.256TrpTrp: 0.256 ± 0.015
0.313TrpTyr: 0.313 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.85TyrAla: 2.85 ± 0.049
0.257TyrCys: 0.257 ± 0.016
1.541TyrAsp: 1.541 ± 0.039
1.02TyrGlu: 1.02 ± 0.028
0.822TyrPhe: 0.822 ± 0.026
2.187TyrGly: 2.187 ± 0.044
0.581TyrHis: 0.581 ± 0.024
0.826TyrIle: 0.826 ± 0.024
0.533TyrLys: 0.533 ± 0.022
2.053TyrLeu: 2.053 ± 0.041
0.4TyrMet: 0.4 ± 0.018
0.722TyrAsn: 0.722 ± 0.028
1.095TyrPro: 1.095 ± 0.033
0.772TyrGln: 0.772 ± 0.029
1.828TyrArg: 1.828 ± 0.039
1.155TyrSer: 1.155 ± 0.031
1.206TyrThr: 1.206 ± 0.037
1.531TyrVal: 1.531 ± 0.037
0.358TyrTrp: 0.358 ± 0.018
0.629TyrTyr: 0.629 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3834 proteins (1291571 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski