Hubei picorna-like virus 71

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJ91|A0A1L3KJ91_9VIRU Uncharacterized protein OS=Hubei picorna-like virus 71 OX=1923155 PE=4 SV=1
MM1 pKa = 7.95ASACKK6 pKa = 9.87NCSPICQGVRR16 pKa = 11.84NCVTTTIEE24 pKa = 4.07SLRR27 pKa = 11.84TNLTALSFVSPTSMQAITALRR48 pKa = 11.84DD49 pKa = 3.45SLLVAKK55 pKa = 10.4GCIEE59 pKa = 3.86SSDD62 pKa = 3.54YY63 pKa = 11.07GRR65 pKa = 11.84ARR67 pKa = 11.84FVLTAGTPTAKK78 pKa = 9.95EE79 pKa = 3.89LSGLPSNTPFIRR91 pKa = 11.84ALAVAIGRR99 pKa = 11.84SYY101 pKa = 11.51SAIQTNAIDD110 pKa = 4.2TLQKK114 pKa = 9.91ICPNEE119 pKa = 3.85QHH121 pKa = 6.02HH122 pKa = 7.23HH123 pKa = 6.13IMYY126 pKa = 10.24QPNDD130 pKa = 4.11DD131 pKa = 4.89PLSCTPWKK139 pKa = 10.04FRR141 pKa = 11.84RR142 pKa = 11.84LIQAGTVDD150 pKa = 3.75RR151 pKa = 11.84ATADD155 pKa = 3.29VFEE158 pKa = 4.55GFEE161 pKa = 4.59NIFLLHH167 pKa = 7.03EE168 pKa = 4.67DD169 pKa = 3.42PGYY172 pKa = 10.53EE173 pKa = 3.91GHH175 pKa = 7.36HH176 pKa = 6.74NITDD180 pKa = 3.87TLRR183 pKa = 11.84TCHH186 pKa = 5.72GTHH189 pKa = 7.08DD190 pKa = 3.55RR191 pKa = 11.84CLYY194 pKa = 10.28CVLGYY199 pKa = 8.69KK200 pKa = 9.5HH201 pKa = 7.01CYY203 pKa = 9.0ASRR206 pKa = 11.84SHH208 pKa = 6.77FVRR211 pKa = 11.84VCGNDD216 pKa = 3.03EE217 pKa = 3.94CAGCYY222 pKa = 9.65FIEE225 pKa = 4.22HH226 pKa = 6.58HH227 pKa = 6.37VLGRR231 pKa = 11.84DD232 pKa = 3.49VNHH235 pKa = 6.21TRR237 pKa = 11.84MIQFYY242 pKa = 10.27KK243 pKa = 9.57FAQMYY248 pKa = 9.02HH249 pKa = 6.61FYY251 pKa = 11.25ANRR254 pKa = 11.84HH255 pKa = 4.14YY256 pKa = 10.96QIDD259 pKa = 4.26EE260 pKa = 4.2DD261 pKa = 4.32KK262 pKa = 11.08MPIPEE267 pKa = 4.38VNTYY271 pKa = 10.5SYY273 pKa = 11.38PLFTYY278 pKa = 9.75RR279 pKa = 11.84HH280 pKa = 5.31TRR282 pKa = 11.84TLYY285 pKa = 7.92TTEE288 pKa = 3.88SQDD291 pKa = 4.09RR292 pKa = 11.84MIEE295 pKa = 3.8NAMNEE300 pKa = 4.02DD301 pKa = 5.33GEE303 pKa = 4.7IEE305 pKa = 3.94WHH307 pKa = 6.56RR308 pKa = 11.84LAEE311 pKa = 4.48SII313 pKa = 5.03

Molecular weight: 35.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJ89|A0A1L3KJ89_9VIRU Calici_coat domain-containing protein OS=Hubei picorna-like virus 71 OX=1923155 PE=4 SV=1
MM1 pKa = 6.63NTKK4 pKa = 8.67TLQWHH9 pKa = 5.11PRR11 pKa = 11.84ARR13 pKa = 11.84IVRR16 pKa = 11.84QSVRR20 pKa = 11.84GYY22 pKa = 8.6VTVSPLPLNHH32 pKa = 6.54SEE34 pKa = 4.57RR35 pKa = 11.84TLQLCPLCLQQVCKK49 pKa = 10.46LLQHH53 pKa = 6.62SEE55 pKa = 4.04TLYY58 pKa = 11.07LLQKK62 pKa = 10.38GALNRR67 pKa = 11.84AIMVEE72 pKa = 4.15LGSYY76 pKa = 10.43LPLEE80 pKa = 4.27PQRR83 pKa = 11.84PRR85 pKa = 11.84NCQGYY90 pKa = 8.53LAILPLYY97 pKa = 10.39AHH99 pKa = 7.33LPLQSDD105 pKa = 3.71DD106 pKa = 3.39HH107 pKa = 5.87TVPFRR112 pKa = 11.84RR113 pKa = 11.84MLSTHH118 pKa = 4.57FRR120 pKa = 11.84KK121 pKa = 9.69YY122 pKa = 11.16VPMNSIITLCTNPMMTHH139 pKa = 5.37FHH141 pKa = 6.06AHH143 pKa = 7.08LGNSDD148 pKa = 3.38DD149 pKa = 4.99SSRR152 pKa = 11.84LEE154 pKa = 3.67QWIEE158 pKa = 3.81QQQMCLKK165 pKa = 10.58DD166 pKa = 3.6SRR168 pKa = 11.84TFSYY172 pKa = 11.05YY173 pKa = 10.27MKK175 pKa = 9.86IQDD178 pKa = 4.06MKK180 pKa = 11.04AITISQIPSALATGPTIDD198 pKa = 4.53AYY200 pKa = 9.65TVSSDD205 pKa = 3.82INTAMQAAATLLEE218 pKa = 4.49FAEE221 pKa = 4.47TMNAQAATSSNTMCLEE237 pKa = 4.2EE238 pKa = 4.11TSTTQEE244 pKa = 3.59

Molecular weight: 27.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3725

244

2408

931.3

105.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.738 ± 0.6

2.013 ± 0.549

5.342 ± 0.595

6.013 ± 0.801

4.268 ± 0.45

5.53 ± 0.597

2.819 ± 0.543

5.691 ± 0.408

6.282 ± 1.429

7.705 ± 0.756

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.872 ± 0.315

5.477 ± 0.746

5.074 ± 0.52

4.591 ± 0.805

4.537 ± 0.454

6.577 ± 0.568

6.899 ± 0.76

5.718 ± 0.744

1.342 ± 0.216

4.51 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski