Rhodococcus phage Harlequin

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rerduovirus; unclassified Rerduovirus

Average proteome isoelectric point is 5.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8ERG1|A0A1D8ERG1_9CAUD Uncharacterized protein OS=Rhodococcus phage Harlequin OX=1897551 GN=SEA_HARLEQUIN_61 PE=4 SV=1
MM1 pKa = 7.64IEE3 pKa = 4.26SKK5 pKa = 10.99SLGEE9 pKa = 3.86VAGFEE14 pKa = 4.52IEE16 pKa = 4.77AGWIYY21 pKa = 11.35DD22 pKa = 3.59DD23 pKa = 5.01VGPDD27 pKa = 3.51FYY29 pKa = 11.57GDD31 pKa = 3.54EE32 pKa = 4.51VYY34 pKa = 11.07DD35 pKa = 4.0DD36 pKa = 3.87QTIEE40 pKa = 4.25AYY42 pKa = 9.81EE43 pKa = 3.88NNEE46 pKa = 3.39WHH48 pKa = 6.38FVVLIVEE55 pKa = 4.27ARR57 pKa = 11.84FNGHH61 pKa = 5.81VMGTAYY67 pKa = 10.87LGGVEE72 pKa = 4.51VGFFPGVEE80 pKa = 4.18EE81 pKa = 4.67PLDD84 pKa = 3.93PLTNSDD90 pKa = 4.46YY91 pKa = 11.04LDD93 pKa = 4.2DD94 pKa = 4.97MIAEE98 pKa = 4.68AVNSARR104 pKa = 11.84TEE106 pKa = 3.99LQEE109 pKa = 4.41TITKK113 pKa = 9.99GLEE116 pKa = 3.77VLLL119 pKa = 4.65

Molecular weight: 13.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8ERE9|A0A1D8ERE9_9CAUD DnaB-like dsDNA helicase OS=Rhodococcus phage Harlequin OX=1897551 GN=SEA_HARLEQUIN_52 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.85SMGKK6 pKa = 9.91AIKK9 pKa = 8.41WTFKK13 pKa = 10.46ATMFLALATVAVIVVLGGLTLAVVGLRR40 pKa = 11.84KK41 pKa = 9.75LL42 pKa = 3.44

Molecular weight: 4.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

14082

42

1115

213.4

23.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.423 ± 0.408

0.817 ± 0.12

6.611 ± 0.207

7.435 ± 0.471

3.394 ± 0.187

8.522 ± 0.392

2.003 ± 0.198

4.602 ± 0.223

3.891 ± 0.195

8.344 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.159

3.565 ± 0.202

4.694 ± 0.223

3.252 ± 0.152

6.086 ± 0.298

5.965 ± 0.305

6.143 ± 0.282

7.897 ± 0.268

1.875 ± 0.171

3.117 ± 0.24
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski