Mycobacterium phage Toaka

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TRE9|A0A5J6TRE9_9CAUD Uncharacterized protein OS=Mycobacterium phage Toaka OX=2599881 GN=57 PE=4 SV=1
MM1 pKa = 7.58PRR3 pKa = 11.84ATYY6 pKa = 11.17VNFVDD11 pKa = 5.26RR12 pKa = 11.84FQLFAGEE19 pKa = 4.42PMLDD23 pKa = 3.3TSDD26 pKa = 3.51GVLVIQYY33 pKa = 10.08PDD35 pKa = 3.31GTSRR39 pKa = 11.84TLNWDD44 pKa = 3.59FVIDD48 pKa = 4.06FYY50 pKa = 11.98YY51 pKa = 8.02MTEE54 pKa = 3.87EE55 pKa = 4.76EE56 pKa = 4.44YY57 pKa = 11.33ADD59 pKa = 3.97TIRR62 pKa = 11.84QFEE65 pKa = 4.28EE66 pKa = 4.9KK67 pKa = 9.79EE68 pKa = 4.13DD69 pKa = 3.68SSEE72 pKa = 3.85

Molecular weight: 8.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TRS6|A0A5J6TRS6_9CAUD Lysin A OS=Mycobacterium phage Toaka OX=2599881 GN=9 PE=4 SV=1
MM1 pKa = 6.82GCVRR5 pKa = 11.84IRR7 pKa = 11.84LPRR10 pKa = 11.84TVSWADD16 pKa = 2.75SDD18 pKa = 4.49RR19 pKa = 11.84RR20 pKa = 11.84QNLPADD26 pKa = 3.55WEE28 pKa = 4.17QRR30 pKa = 11.84RR31 pKa = 11.84LHH33 pKa = 6.68ILVDD37 pKa = 3.75ADD39 pKa = 3.46WRR41 pKa = 11.84CEE43 pKa = 3.51IKK45 pKa = 10.15MAGCTSVATDD55 pKa = 2.94VDD57 pKa = 4.37HH58 pKa = 7.64IKK60 pKa = 10.61RR61 pKa = 11.84GNDD64 pKa = 2.92HH65 pKa = 6.95SYY67 pKa = 11.02GNLRR71 pKa = 11.84AACGWCHH78 pKa = 6.24DD79 pKa = 4.39RR80 pKa = 11.84KK81 pKa = 10.73SSAEE85 pKa = 3.84GVSRR89 pKa = 11.84RR90 pKa = 11.84RR91 pKa = 11.84EE92 pKa = 3.6LKK94 pKa = 10.2ARR96 pKa = 11.84RR97 pKa = 11.84KK98 pKa = 9.71RR99 pKa = 11.84PPEE102 pKa = 3.6RR103 pKa = 11.84HH104 pKa = 5.7PGRR107 pKa = 11.84RR108 pKa = 3.38

Molecular weight: 12.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

16614

40

975

174.9

19.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.172 ± 0.395

0.807 ± 0.105

6.242 ± 0.205

6.501 ± 0.303

3.365 ± 0.188

8.421 ± 0.47

1.908 ± 0.167

5.002 ± 0.159

4.629 ± 0.202

8.168 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.462 ± 0.171

3.539 ± 0.174

5.212 ± 0.226

3.509 ± 0.219

6.23 ± 0.304

5.736 ± 0.233

6.302 ± 0.255

7.03 ± 0.233

1.944 ± 0.125

2.823 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski