Mycoplasma hominis (strain ATCC 23114 / NBRC 14850 / NCTC 10111 / PG21)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma hominis

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 529 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D1J8M6|D1J8M6_MYCHP Uncharacterized protein OS=Mycoplasma hominis (strain ATCC 23114 / NBRC 14850 / NCTC 10111 / PG21) OX=347256 GN=MHO_4380 PE=4 SV=1
MM1 pKa = 7.39LNKK4 pKa = 9.74IASHH8 pKa = 6.25CWSFSFAISSSFIIFSYY25 pKa = 10.04WFIISPADD33 pKa = 3.93GIFFAISSNCFIFDD47 pKa = 3.97LTCCCFDD54 pKa = 3.63VNFEE58 pKa = 4.49HH59 pKa = 8.05DD60 pKa = 3.07IATIGNEE67 pKa = 4.09IKK69 pKa = 10.65EE70 pKa = 4.31LAPKK74 pKa = 9.39IDD76 pKa = 4.28KK77 pKa = 10.68IIFLLFFIEE86 pKa = 4.54YY87 pKa = 9.3PLSLFNEE94 pKa = 4.49LLDD97 pKa = 4.27MIIININLIIFF108 pKa = 4.39

Molecular weight: 12.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D1J813|D1J813_MYCHP Uncharacterized protein OS=Mycoplasma hominis (strain ATCC 23114 / NBRC 14850 / NCTC 10111 / PG21) OX=347256 GN=MHO_2260 PE=4 SV=1
MM1 pKa = 7.61ADD3 pKa = 2.98KK4 pKa = 10.79VRR6 pKa = 11.84YY7 pKa = 9.12YY8 pKa = 11.35GLGRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 10.01SSVARR19 pKa = 11.84VYY21 pKa = 10.59ILPGSGNLIINGIVAKK37 pKa = 10.09EE38 pKa = 3.92YY39 pKa = 10.92LNSEE43 pKa = 4.17ILIKK47 pKa = 10.8DD48 pKa = 3.47ALSPFAVTEE57 pKa = 3.99TANQFDD63 pKa = 3.68VFANVNGGGLTGQAGAIRR81 pKa = 11.84LGIARR86 pKa = 11.84ALLEE90 pKa = 4.27ASNNEE95 pKa = 3.78YY96 pKa = 10.57RR97 pKa = 11.84NKK99 pKa = 10.73LKK101 pKa = 10.82DD102 pKa = 3.29AGFLTRR108 pKa = 11.84DD109 pKa = 3.24ARR111 pKa = 11.84VKK113 pKa = 9.75EE114 pKa = 4.1RR115 pKa = 11.84KK116 pKa = 9.77KK117 pKa = 10.69FGLRR121 pKa = 11.84KK122 pKa = 9.46ARR124 pKa = 11.84RR125 pKa = 11.84ARR127 pKa = 11.84QFSKK131 pKa = 10.87RR132 pKa = 3.51

Molecular weight: 14.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

529

0

529

196644

37

2791

371.7

42.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.299 ± 0.087

0.699 ± 0.032

5.484 ± 0.093

6.981 ± 0.113

5.207 ± 0.091

4.541 ± 0.113

1.314 ± 0.041

10.084 ± 0.107

10.625 ± 0.134

9.542 ± 0.089

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.71 ± 0.05

8.303 ± 0.142

2.626 ± 0.06

3.356 ± 0.072

2.808 ± 0.075

6.695 ± 0.087

4.673 ± 0.065

5.011 ± 0.076

0.857 ± 0.03

4.185 ± 0.073
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski