Flavobacteria bacterium MS024-3C

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; unclassified Flavobacteriia

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1382 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0BMI9|C0BMI9_9BACT AIR synthase OS=Flavobacteria bacterium MS024-3C OX=487797 GN=Flav3CDRAFT_0590 PE=3 SV=1
FF1 pKa = 7.55PLDD4 pKa = 3.6PNEE7 pKa = 4.24WADD10 pKa = 4.04ADD12 pKa = 3.87NDD14 pKa = 5.06GIGDD18 pKa = 4.3NADD21 pKa = 3.37TDD23 pKa = 4.3DD24 pKa = 4.76NNDD27 pKa = 3.49GFDD30 pKa = 3.78DD31 pKa = 4.62DD32 pKa = 4.68KK33 pKa = 11.83LFVSGLLTPGSSGLEE48 pKa = 4.15DD49 pKa = 2.88TWKK52 pKa = 10.7VINIEE57 pKa = 4.04QYY59 pKa = 9.17PNARR63 pKa = 11.84VIIYY67 pKa = 9.52NRR69 pKa = 11.84NAQEE73 pKa = 4.02VFSVLGYY80 pKa = 9.79KK81 pKa = 10.36NNWRR85 pKa = 11.84GTYY88 pKa = 9.63KK89 pKa = 10.65GSSNLLPAGSYY100 pKa = 10.77YY101 pKa = 10.99YY102 pKa = 11.09VVDD105 pKa = 4.84LNNGEE110 pKa = 4.11KK111 pKa = 10.38PITGWLYY118 pKa = 9.61LTYY121 pKa = 11.06

Molecular weight: 13.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0BN99|C0BN99_9BACT Indole-3-glycerol phosphate synthase OS=Flavobacteria bacterium MS024-3C OX=487797 GN=trpC PE=3 SV=1
MM1 pKa = 7.79LYY3 pKa = 10.62KK4 pKa = 10.73LLYY7 pKa = 9.56FFQRR11 pKa = 11.84HH12 pKa = 5.18GFTVCEE18 pKa = 4.47RR19 pKa = 11.84IAEE22 pKa = 4.19RR23 pKa = 11.84MGIRR27 pKa = 11.84ARR29 pKa = 11.84VVRR32 pKa = 11.84TSFIYY37 pKa = 10.42LAFVTLGFGFALYY50 pKa = 10.8LFMAFWIRR58 pKa = 11.84IKK60 pKa = 10.92DD61 pKa = 3.52LLFAKK66 pKa = 10.13RR67 pKa = 11.84NSVFDD72 pKa = 3.74LL73 pKa = 4.13

Molecular weight: 8.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1382

0

1382

469315

30

4666

339.6

37.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.02 ± 0.072

0.706 ± 0.024

5.217 ± 0.071

6.032 ± 0.068

5.087 ± 0.055

6.974 ± 0.069

1.946 ± 0.038

7.177 ± 0.063

7.028 ± 0.094

9.829 ± 0.094

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.342 ± 0.037

5.161 ± 0.066

3.808 ± 0.036

3.684 ± 0.038

3.412 ± 0.045

6.648 ± 0.066

5.655 ± 0.113

6.462 ± 0.047

1.076 ± 0.025

3.734 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski