Owenweeksia hongkongensis (strain DSM 17368 / CIP 108786 / JCM 12287 / NRRL B-23963 / UST20020801)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Schleiferiaceae; Owenweeksia; Owenweeksia hongkongensis

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3471 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8R6A2|G8R6A2_OWEHD Uncharacterized protein OS=Owenweeksia hongkongensis (strain DSM 17368 / CIP 108786 / JCM 12287 / NRRL B-23963 / UST20020801) OX=926562 GN=Oweho_2351 PE=4 SV=1
MM1 pKa = 7.36NNYY4 pKa = 8.01TLSVNKK10 pKa = 10.0VSFAKK15 pKa = 10.86DD16 pKa = 3.11NGDD19 pKa = 3.03VSLAITLKK27 pKa = 10.84GSFPQPVNSIGNLVNDD43 pKa = 5.3TIQFDD48 pKa = 3.99LTSEE52 pKa = 4.09SGATVDD58 pKa = 3.45EE59 pKa = 4.59AVFTFQMGEE68 pKa = 4.26GYY70 pKa = 9.79PGDD73 pKa = 3.92FEE75 pKa = 5.54FINYY79 pKa = 7.0PQGYY83 pKa = 9.2GDD85 pKa = 4.23APINGDD91 pKa = 3.32VNADD95 pKa = 3.54NNEE98 pKa = 3.86EE99 pKa = 4.15CDD101 pKa = 4.93FEE103 pKa = 6.96LGDD106 pKa = 4.75CVWGVNPTGGLGSGEE121 pKa = 4.12GKK123 pKa = 10.16SLQVSVKK130 pKa = 9.58KK131 pKa = 10.8KK132 pKa = 10.16PGVTSYY138 pKa = 10.57EE139 pKa = 3.69ISSFLLTEE147 pKa = 4.16VEE149 pKa = 4.11ISGAMQLSAVMTLHH163 pKa = 6.97EE164 pKa = 4.33NAEE167 pKa = 4.24DD168 pKa = 3.69QLEE171 pKa = 4.37ATCEE175 pKa = 3.86EE176 pKa = 4.43LVNFTEE182 pKa = 4.89IGHH185 pKa = 7.02ALPIVTINEE194 pKa = 4.51SGDD197 pKa = 3.42STPRR201 pKa = 11.84SIGVIYY207 pKa = 9.17VNCC210 pKa = 4.27

Molecular weight: 22.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8R2M1|G8R2M1_OWEHD 50S ribosomal protein L15 OS=Owenweeksia hongkongensis (strain DSM 17368 / CIP 108786 / JCM 12287 / NRRL B-23963 / UST20020801) OX=926562 GN=rplO PE=3 SV=1
MM1 pKa = 7.61GVRR4 pKa = 11.84KK5 pKa = 9.94LNPVTPGQRR14 pKa = 11.84FRR16 pKa = 11.84IVNDD20 pKa = 2.91NSTITAAKK28 pKa = 9.34PEE30 pKa = 3.89KK31 pKa = 10.59SLVKK35 pKa = 10.44GKK37 pKa = 10.74SKK39 pKa = 10.99GGGRR43 pKa = 11.84NNTGKK48 pKa = 8.11MTIRR52 pKa = 11.84NVGGGHH58 pKa = 4.41KK59 pKa = 9.64QKK61 pKa = 10.77YY62 pKa = 8.52RR63 pKa = 11.84VVDD66 pKa = 4.69FKK68 pKa = 11.02RR69 pKa = 11.84DD70 pKa = 3.59KK71 pKa = 10.62EE72 pKa = 4.39GVPATVKK79 pKa = 10.53AVEE82 pKa = 4.0YY83 pKa = 10.59DD84 pKa = 3.47PNRR87 pKa = 11.84TARR90 pKa = 11.84IALLYY95 pKa = 10.03YY96 pKa = 10.51ADD98 pKa = 3.69GEE100 pKa = 4.31KK101 pKa = 10.29RR102 pKa = 11.84YY103 pKa = 9.45MIAPNGLQVGQTVVSGRR120 pKa = 11.84EE121 pKa = 3.68AAPEE125 pKa = 3.92IGNAMYY131 pKa = 10.77LSDD134 pKa = 4.36IPLGTVISCIEE145 pKa = 3.83MRR147 pKa = 11.84PGQGAVIARR156 pKa = 11.84SAGSFAQLAARR167 pKa = 11.84DD168 pKa = 3.75GKK170 pKa = 10.61YY171 pKa = 10.67AIIKK175 pKa = 9.48LPSGEE180 pKa = 3.93TRR182 pKa = 11.84MILITCMAMIGAVSNSDD199 pKa = 3.18NAQEE203 pKa = 4.26VSGKK207 pKa = 10.27AGRR210 pKa = 11.84KK211 pKa = 7.13RR212 pKa = 11.84WQGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84VRR221 pKa = 11.84GVAMNPVDD229 pKa = 3.68HH230 pKa = 7.13PMGGGEE236 pKa = 3.89GRR238 pKa = 11.84ASGGHH243 pKa = 5.13PRR245 pKa = 11.84SRR247 pKa = 11.84NGIPAKK253 pKa = 10.14GYY255 pKa = 7.49KK256 pKa = 8.5TRR258 pKa = 11.84APKK261 pKa = 10.32KK262 pKa = 9.2EE263 pKa = 3.77SSKK266 pKa = 11.08YY267 pKa = 9.4IIEE270 pKa = 4.11RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.1KK274 pKa = 9.82

Molecular weight: 29.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3471

0

3471

1212283

33

4841

349.3

39.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.896 ± 0.039

0.814 ± 0.015

5.697 ± 0.033

6.55 ± 0.059

4.986 ± 0.03

7.016 ± 0.047

1.843 ± 0.022

6.974 ± 0.034

6.612 ± 0.062

9.235 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.025

5.561 ± 0.038

3.725 ± 0.025

3.492 ± 0.025

3.692 ± 0.031

6.983 ± 0.051

5.796 ± 0.056

6.446 ± 0.034

1.196 ± 0.017

4.058 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski