Mediterranea sp. An20

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Mediterranea; unclassified Mediterranea

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3285 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4HYG0|A0A1Y4HYG0_9BACT Lipid-A-disaccharide synthase OS=Mediterranea sp. An20 OX=1965586 GN=B5F34_02140 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 9.93MKK4 pKa = 10.62SKK6 pKa = 10.88LFGLGAKK13 pKa = 8.32MALALLAVGMTVTSCYY29 pKa = 10.45DD30 pKa = 3.35SEE32 pKa = 4.48NVDD35 pKa = 4.73IIQPDD40 pKa = 3.45QPVAPKK46 pKa = 10.58YY47 pKa = 10.45YY48 pKa = 9.65IAGNITDD55 pKa = 4.96AEE57 pKa = 4.4TGDD60 pKa = 3.4IVTNATVTIDD70 pKa = 3.47GTAVTLTNGAFNQEE84 pKa = 4.21VSVGDD89 pKa = 3.77AGASFVVAVVADD101 pKa = 4.68GYY103 pKa = 10.93RR104 pKa = 11.84DD105 pKa = 3.38VSRR108 pKa = 11.84TIFFPAMGNGTVTVGNADD126 pKa = 3.58FALVGVTASDD136 pKa = 3.98LLEE139 pKa = 4.52EE140 pKa = 6.07DD141 pKa = 4.66PLQDD145 pKa = 3.65NYY147 pKa = 10.66PVSDD151 pKa = 3.86EE152 pKa = 4.77EE153 pKa = 4.34YY154 pKa = 9.45TLLKK158 pKa = 10.21TEE160 pKa = 4.8LEE162 pKa = 4.29NVLPEE167 pKa = 4.38SVVLTTDD174 pKa = 2.96EE175 pKa = 5.09DD176 pKa = 4.55GNIVATYY183 pKa = 10.12AAEE186 pKa = 4.06EE187 pKa = 4.36VTNSAIGEE195 pKa = 4.23DD196 pKa = 4.11LEE198 pKa = 4.4ITAPYY203 pKa = 10.29YY204 pKa = 10.93SGFASTISQDD214 pKa = 3.32ADD216 pKa = 4.18DD217 pKa = 5.17IFTKK221 pKa = 10.76ALTDD225 pKa = 3.5GRR227 pKa = 11.84IWNASASNVLGLPYY241 pKa = 10.76GLTLGEE247 pKa = 4.33RR248 pKa = 11.84SVILPGRR255 pKa = 11.84PGCSISTWTLTQVFAVEE272 pKa = 4.14PLSFGGVVGTVTYY285 pKa = 10.27QSNWVITFSYY295 pKa = 9.8EE296 pKa = 3.99SHH298 pKa = 7.7DD299 pKa = 3.38GHH301 pKa = 7.15DD302 pKa = 3.05WHH304 pKa = 7.68DD305 pKa = 3.49NHH307 pKa = 7.24GSVNGAGGGNNNMGYY322 pKa = 10.85

Molecular weight: 33.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4HLE3|A0A1Y4HLE3_9BACT Probable dual-specificity RNA methyltransferase RlmN OS=Mediterranea sp. An20 OX=1965586 GN=rlmN PE=3 SV=1
MM1 pKa = 7.33NNKK4 pKa = 9.31EE5 pKa = 4.15LIRR8 pKa = 11.84RR9 pKa = 11.84NEE11 pKa = 3.22ISRR14 pKa = 11.84WKK16 pKa = 10.52NEE18 pKa = 3.49RR19 pKa = 11.84KK20 pKa = 9.49AAALHH25 pKa = 6.28GISSSFLSHH34 pKa = 7.06SSLKK38 pKa = 10.28ICFLLLLLVGLTSCRR53 pKa = 11.84TTAPNLDD60 pKa = 3.2YY61 pKa = 10.66RR62 pKa = 11.84ALVRR66 pKa = 11.84ASNRR70 pKa = 11.84LGMDD74 pKa = 3.43IGPKK78 pKa = 10.03DD79 pKa = 3.39NHH81 pKa = 6.26ALYY84 pKa = 10.9LEE86 pKa = 4.19AAEE89 pKa = 4.67WIGVPYY95 pKa = 10.56RR96 pKa = 11.84SGGQSKK102 pKa = 10.73RR103 pKa = 11.84GTDD106 pKa = 3.32CSGFARR112 pKa = 11.84QIYY115 pKa = 7.55KK116 pKa = 9.67TIYY119 pKa = 8.36RR120 pKa = 11.84TEE122 pKa = 4.2LPRR125 pKa = 11.84STSEE129 pKa = 3.72QVKK132 pKa = 8.59EE133 pKa = 4.03GRR135 pKa = 11.84RR136 pKa = 11.84VRR138 pKa = 11.84RR139 pKa = 11.84NKK141 pKa = 10.1LRR143 pKa = 11.84EE144 pKa = 3.7GDD146 pKa = 3.7LVFFHH151 pKa = 6.76GSRR154 pKa = 11.84RR155 pKa = 11.84RR156 pKa = 11.84RR157 pKa = 11.84VSHH160 pKa = 5.48VGIYY164 pKa = 10.65LKK166 pKa = 10.65DD167 pKa = 3.42GKK169 pKa = 10.1FVHH172 pKa = 7.01ASTSRR177 pKa = 11.84GVIVSRR183 pKa = 11.84LDD185 pKa = 3.18EE186 pKa = 4.7EE187 pKa = 4.71YY188 pKa = 10.13WDD190 pKa = 3.85EE191 pKa = 3.42HH192 pKa = 4.26WMRR195 pKa = 11.84GRR197 pKa = 11.84RR198 pKa = 11.84IRR200 pKa = 3.95

Molecular weight: 23.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3285

0

3285

1098706

34

1932

334.5

37.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.63 ± 0.044

1.399 ± 0.018

5.674 ± 0.027

6.665 ± 0.044

4.388 ± 0.029

6.909 ± 0.036

2.097 ± 0.021

6.137 ± 0.039

5.686 ± 0.038

9.67 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.727 ± 0.02

4.412 ± 0.034

3.937 ± 0.023

3.708 ± 0.025

5.613 ± 0.036

5.633 ± 0.032

5.516 ± 0.03

6.578 ± 0.033

1.295 ± 0.016

4.327 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski