Xingshan nematode virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KF94|A0A1L3KF94_9VIRU RNA-directed RNA polymerase OS=Xingshan nematode virus 5 OX=1923764 PE=4 SV=1
MM1 pKa = 7.52CPPWRR6 pKa = 11.84HH7 pKa = 5.43KK8 pKa = 10.56FSRR11 pKa = 11.84SPAGPRR17 pKa = 11.84ARR19 pKa = 11.84TQEE22 pKa = 4.35SATEE26 pKa = 4.07TPGFEE31 pKa = 4.24EE32 pKa = 4.54VGWSYY37 pKa = 11.47GVYY40 pKa = 10.36SRR42 pKa = 11.84MRR44 pKa = 11.84EE45 pKa = 3.91PTINPLMEE53 pKa = 4.28EE54 pKa = 3.96ALRR57 pKa = 11.84PYY59 pKa = 11.1AEE61 pKa = 4.46LFQYY65 pKa = 8.87TGQYY69 pKa = 9.32AWPPRR74 pKa = 11.84GPEE77 pKa = 3.76AEE79 pKa = 3.88RR80 pKa = 11.84VSLRR84 pKa = 11.84VHH86 pKa = 5.6ARR88 pKa = 11.84LRR90 pKa = 11.84AEE92 pKa = 4.22ILAGMQEE99 pKa = 4.05PTHH102 pKa = 7.83DD103 pKa = 3.72EE104 pKa = 4.39EE105 pKa = 4.47EE106 pKa = 4.22WLVAQTVKK114 pKa = 10.71AYY116 pKa = 10.46RR117 pKa = 11.84PARR120 pKa = 11.84WEE122 pKa = 3.85IPDD125 pKa = 5.45DD126 pKa = 3.91FLSWTHH132 pKa = 5.68FMRR135 pKa = 11.84VVARR139 pKa = 11.84LEE141 pKa = 4.46RR142 pKa = 11.84NSSPGWPYY150 pKa = 10.15CRR152 pKa = 11.84QYY154 pKa = 10.72GTLKK158 pKa = 10.63LWIGEE163 pKa = 4.02ILDD166 pKa = 4.08PDD168 pKa = 3.64EE169 pKa = 6.07AGIRR173 pKa = 11.84MLWDD177 pKa = 3.77LVQGRR182 pKa = 11.84LRR184 pKa = 11.84GDD186 pKa = 3.27EE187 pKa = 3.96AHH189 pKa = 7.79PIRR192 pKa = 11.84LFVKK196 pKa = 9.93PEE198 pKa = 3.49PHH200 pKa = 7.03KK201 pKa = 10.57ISKK204 pKa = 9.33LQEE207 pKa = 3.27GRR209 pKa = 11.84YY210 pKa = 9.66RR211 pKa = 11.84LISSVNVVDD220 pKa = 4.41QVIDD224 pKa = 3.35HH225 pKa = 6.56MLFDD229 pKa = 3.88MQNDD233 pKa = 3.73RR234 pKa = 11.84EE235 pKa = 4.46IAVHH239 pKa = 6.82LDD241 pKa = 3.15IPSKK245 pKa = 10.42PGWSPARR252 pKa = 11.84GGWRR256 pKa = 11.84MLQQFEE262 pKa = 4.56TAVSCDD268 pKa = 3.02KK269 pKa = 11.31SLWDD273 pKa = 3.95WGMPWWVARR282 pKa = 11.84LDD284 pKa = 3.92LKK286 pKa = 11.21VRR288 pKa = 11.84ASLCMNMQTAWMEE301 pKa = 3.92LAEE304 pKa = 4.04RR305 pKa = 11.84RR306 pKa = 11.84YY307 pKa = 10.3RR308 pKa = 11.84EE309 pKa = 4.19LYY311 pKa = 9.78QEE313 pKa = 4.15ARR315 pKa = 11.84FITSDD320 pKa = 3.46GQIFAQKK327 pKa = 9.57TGGLQKK333 pKa = 10.35SGCVNTISTNSRR345 pKa = 11.84GQYY348 pKa = 9.06LLHH351 pKa = 6.43MLACLRR357 pKa = 11.84SGEE360 pKa = 4.67DD361 pKa = 3.02HH362 pKa = 6.16TQCFWAMGDD371 pKa = 3.46DD372 pKa = 3.98TLQEE376 pKa = 4.11RR377 pKa = 11.84ASEE380 pKa = 4.52KK381 pKa = 10.68YY382 pKa = 9.95CAEE385 pKa = 4.09LGKK388 pKa = 10.39LCRR391 pKa = 11.84LKK393 pKa = 10.75EE394 pKa = 4.06VTPGIVFAGTRR405 pKa = 11.84LDD407 pKa = 3.59SEE409 pKa = 4.95GVHH412 pKa = 6.79PEE414 pKa = 4.13YY415 pKa = 10.51CSKK418 pKa = 10.91HH419 pKa = 5.66YY420 pKa = 8.08FTLLHH425 pKa = 6.64KK426 pKa = 10.54SQEE429 pKa = 4.28AVPDD433 pKa = 3.91TLSSYY438 pKa = 10.43VLLYY442 pKa = 11.0ARR444 pKa = 11.84DD445 pKa = 3.66QRR447 pKa = 11.84LEE449 pKa = 3.76ALLEE453 pKa = 4.02VARR456 pKa = 11.84RR457 pKa = 11.84LVPGVWRR464 pKa = 11.84SAVEE468 pKa = 3.61LRR470 pKa = 11.84GWYY473 pKa = 9.88DD474 pKa = 3.38GLIDD478 pKa = 3.96

Molecular weight: 55.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KF94|A0A1L3KF94_9VIRU RNA-directed RNA polymerase OS=Xingshan nematode virus 5 OX=1923764 PE=4 SV=1
MM1 pKa = 7.27STQQGNRR8 pKa = 11.84RR9 pKa = 11.84VRR11 pKa = 11.84RR12 pKa = 11.84LRR14 pKa = 11.84NTVAALQQQLAQMQVQPVVQQTGGRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84AAAIAAAQVSTAPAATSLVVTTPPAPTLRR77 pKa = 11.84VTGTDD82 pKa = 3.06WLDD85 pKa = 3.58SEE87 pKa = 5.8AVGSMMQDD95 pKa = 2.86GWPVFEE101 pKa = 5.96LDD103 pKa = 4.57LNPTQFPGTHH113 pKa = 6.5LAALASGFEE122 pKa = 4.14KK123 pKa = 11.05YY124 pKa = 9.86RR125 pKa = 11.84FNRR128 pKa = 11.84LRR130 pKa = 11.84VLVSTRR136 pKa = 11.84APTTCGGGFIAGITPDD152 pKa = 3.38VYY154 pKa = 10.85QEE156 pKa = 4.03LVRR159 pKa = 11.84GIAGKK164 pKa = 10.3RR165 pKa = 11.84YY166 pKa = 9.01VRR168 pKa = 11.84SLQGAVSSSWWQPATLTMPCGVEE191 pKa = 3.73WLFTSPRR198 pKa = 11.84AEE200 pKa = 3.88RR201 pKa = 11.84SRR203 pKa = 11.84SSAGRR208 pKa = 11.84LFVVIDD214 pKa = 3.75GLPTVDD220 pKa = 5.0KK221 pKa = 11.23INVTLQLEE229 pKa = 4.28WDD231 pKa = 3.65ISLMAPAVPLEE242 pKa = 4.54HH243 pKa = 7.23KK244 pKa = 10.7SHH246 pKa = 6.86GDD248 pKa = 3.05WKK250 pKa = 10.72IPACTWVYY258 pKa = 11.09DD259 pKa = 3.69AAYY262 pKa = 9.17PNWASFKK269 pKa = 10.88AAGTPLDD276 pKa = 3.71EE277 pKa = 4.18WWNNSRR283 pKa = 11.84WSTLYY288 pKa = 10.58LVEE291 pKa = 4.64PGFMVGEE298 pKa = 4.21SPVRR302 pKa = 11.84VIIPHH307 pKa = 6.88DD308 pKa = 3.9FGGSDD313 pKa = 3.65HH314 pKa = 6.88GFLAYY319 pKa = 8.98STLYY323 pKa = 9.98AAKK326 pKa = 9.12TDD328 pKa = 3.56AAGTRR333 pKa = 11.84RR334 pKa = 11.84VKK336 pKa = 9.64VTANVDD342 pKa = 3.1VGPQHH347 pKa = 7.25IIEE350 pKa = 4.57IGPVNFF356 pKa = 4.46

Molecular weight: 39.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1326

356

492

442.0

49.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.824 ± 0.741

1.584 ± 0.268

4.223 ± 0.448

6.938 ± 1.136

3.092 ± 0.175

8.22 ± 0.943

2.036 ± 0.33

3.394 ± 0.168

3.922 ± 0.865

9.351 ± 0.562

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.941 ± 0.325

1.735 ± 0.35

6.184 ± 0.189

3.922 ± 0.723

8.974 ± 0.112

5.354 ± 0.728

5.204 ± 0.853

7.617 ± 0.827

3.017 ± 0.626

3.469 ± 0.399
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski