Streptomyces phage LibertyBell

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385DPG4|A0A385DPG4_9CAUD Uncharacterized protein OS=Streptomyces phage LibertyBell OX=2301574 GN=79 PE=4 SV=1
MM1 pKa = 7.48TKK3 pKa = 10.44LAWDD7 pKa = 3.46QSGEE11 pKa = 4.0RR12 pKa = 11.84LFEE15 pKa = 4.37SGVDD19 pKa = 3.24RR20 pKa = 11.84GVLYY24 pKa = 10.38IPNASGVYY32 pKa = 9.69NNGYY36 pKa = 9.32AWNGLTAVTEE46 pKa = 4.43SPSGAEE52 pKa = 4.33SNPQYY57 pKa = 11.32ADD59 pKa = 3.31NIKK62 pKa = 10.35YY63 pKa = 10.59LNLVSAEE70 pKa = 4.09EE71 pKa = 3.98FGGTIEE77 pKa = 4.52AFTYY81 pKa = 10.37PEE83 pKa = 4.59EE84 pKa = 4.14FAQCDD89 pKa = 3.88GTALPTPGVAVGQQARR105 pKa = 11.84KK106 pKa = 7.85TFGLSYY112 pKa = 8.99RR113 pKa = 11.84TKK115 pKa = 10.66VGNDD119 pKa = 3.35LAGQDD124 pKa = 3.58HH125 pKa = 7.45GYY127 pKa = 10.23KK128 pKa = 9.61IHH130 pKa = 6.78LVYY133 pKa = 10.45GALAAPSEE141 pKa = 4.27KK142 pKa = 10.45AYY144 pKa = 9.68ATINDD149 pKa = 3.9SPEE152 pKa = 4.66AITFSWEE159 pKa = 3.78FTTTPVEE166 pKa = 4.0VGEE169 pKa = 4.33ISGVTYY175 pKa = 10.62RR176 pKa = 11.84PTATITIDD184 pKa = 3.5STKK187 pKa = 10.51VEE189 pKa = 4.5AGDD192 pKa = 3.66LATLEE197 pKa = 4.07EE198 pKa = 4.6FLYY201 pKa = 9.05GTAGSDD207 pKa = 3.42PSLPSPQAVMAMFTGSVLVATPTQPTYY234 pKa = 11.06DD235 pKa = 3.71AGTNTITFPVIAGVDD250 pKa = 3.47YY251 pKa = 10.4FVNDD255 pKa = 3.9EE256 pKa = 4.51LQTAPLIITEE266 pKa = 4.18DD267 pKa = 3.91TIVTARR273 pKa = 11.84PKK275 pKa = 10.04IGYY278 pKa = 8.53KK279 pKa = 10.21FPPNVDD285 pKa = 3.83PDD287 pKa = 3.63WLIVFSS293 pKa = 4.38

Molecular weight: 31.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385DRH9|A0A385DRH9_9CAUD Uncharacterized protein OS=Streptomyces phage LibertyBell OX=2301574 GN=61 PE=4 SV=1
MM1 pKa = 7.94RR2 pKa = 11.84IYY4 pKa = 10.68FSGLRR9 pKa = 11.84TWVIRR14 pKa = 11.84PITVFSVKK22 pKa = 10.27GGVMTEE28 pKa = 3.95DD29 pKa = 5.77GILGWQVGPLRR40 pKa = 11.84IIFWDD45 pKa = 3.46RR46 pKa = 11.84MKK48 pKa = 11.19

Molecular weight: 5.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

16252

32

1841

205.7

22.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.125 ± 0.578

0.461 ± 0.084

6.018 ± 0.255

6.645 ± 0.353

3.815 ± 0.235

7.685 ± 0.382

1.772 ± 0.172

5.772 ± 0.199

6.313 ± 0.354

7.901 ± 0.427

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.824 ± 0.156

4.123 ± 0.133

4.024 ± 0.332

3.403 ± 0.198

5.722 ± 0.381

5.845 ± 0.194

6.602 ± 0.365

7.138 ± 0.198

1.409 ± 0.143

3.403 ± 0.312
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski